Le Yang, Diantong Ma, Fang Wang, Li Liu, Lin Chen, Xiahong He, Junrong Tang, Jia Deng
{"title":"羧甲基纤维素诱导的月桂隐球菌可提高葡萄柚的抗病性，并调节苯丙氨酸和活性氧代谢","authors":"Le Yang, Diantong Ma, Fang Wang, Li Liu, Lin Chen, Xiahong He, Junrong Tang, Jia Deng","doi":"10.1016/j.foodchem.2024.141955","DOIUrl":null,"url":null,"abstract":"Green mould disease poses a significant threat to the citrus industry. <em>Cryptococcus laurentii</em> can stimulate the fruit defence system, whereas the use of antagonistic yeast alone demonstrates limited efficacy. This study investigated the molecular mechanisms of <em>C. laurentii</em> cultured with carboxymethyl cellulose (CMC<img alt=\"single bond\" src=\"https://sdfestaticassets-us-east-1.sciencedirectassets.com/shared-assets/55/entities/sbnd.gif\" style=\"vertical-align:middle\"/><em>C. laurentii</em>), and evaluated the effects of CMC<img alt=\"single bond\" src=\"https://sdfestaticassets-us-east-1.sciencedirectassets.com/shared-assets/55/entities/sbnd.gif\" style=\"vertical-align:middle\"/><em>C. laurentii</em> on phenylpropane and reactive oxygen metabolism in grapefruit fruit. Transcriptome analysis revealed that the upregulation of gene expression associated with yeast growth and antagonistic ability occurred in CMC<img alt=\"single bond\" src=\"https://sdfestaticassets-us-east-1.sciencedirectassets.com/shared-assets/55/entities/sbnd.gif\" style=\"vertical-align:middle\"/><em>C. laurentii</em> after 72 h cultivation. Meanwhile, CMC<img alt=\"single bond\" src=\"https://sdfestaticassets-us-east-1.sciencedirectassets.com/shared-assets/55/entities/sbnd.gif\" style=\"vertical-align:middle\"/><em>C. laurentii</em> reduced lesion diameter and disease incidence in fruit. This treatment promoted phenylpropane metabolism by activating PAL, C4H, 4CL, POD, and PPO and increasing the secondary metabolites. CMC<img alt=\"single bond\" src=\"https://sdfestaticassets-us-east-1.sciencedirectassets.com/shared-assets/55/entities/sbnd.gif\" style=\"vertical-align:middle\"/><em>C. laurentii</em> also activated the AsA-GSH cycle, enhanced the activities of SOD and CAT, and reduced the accumulation of H<sub>2</sub>O<sub>2</sub> and O<sub>2</sub><sup>•-</sup>. The results suggested that CMC<img alt=\"single bond\" src=\"https://sdfestaticassets-us-east-1.sciencedirectassets.com/shared-assets/55/entities/sbnd.gif\" style=\"vertical-align:middle\"/><em>C. laurentii</em> maintained high postharvest fruit quality in grapefruit fruit by elevating the phenylpropane and reactive oxygen metabolism.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Carboxymethyl cellulose-induced Cryptococcus laurentii improves disease resistance and regulates phenylpropane and reactive oxygen metabolism in grapefruit\",\"authors\":\"Le Yang, Diantong Ma, Fang Wang, Li Liu, Lin Chen, Xiahong He, Junrong Tang, Jia Deng\",\"doi\":\"10.1016/j.foodchem.2024.141955\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Green mould disease poses a significant threat to the citrus industry. <em>Cryptococcus laurentii</em> can stimulate the fruit defence system, whereas the use of antagonistic yeast alone demonstrates limited efficacy. This study investigated the molecular mechanisms of <em>C. laurentii</em> cultured with carboxymethyl cellulose (CMC<img alt=\\\"single bond\\\" src=\\\"https://sdfestaticassets-us-east-1.sciencedirectassets.com/shared-assets/55/entities/sbnd.gif\\\" style=\\\"vertical-align:middle\\\"/><em>C. laurentii</em>), and evaluated the effects of CMC<img alt=\\\"single bond\\\" src=\\\"https://sdfestaticassets-us-east-1.sciencedirectassets.com/shared-assets/55/entities/sbnd.gif\\\" style=\\\"vertical-align:middle\\\"/><em>C. laurentii</em> on phenylpropane and reactive oxygen metabolism in grapefruit fruit. Transcriptome analysis revealed that the upregulation of gene expression associated with yeast growth and antagonistic ability occurred in CMC<img alt=\\\"single bond\\\" src=\\\"https://sdfestaticassets-us-east-1.sciencedirectassets.com/shared-assets/55/entities/sbnd.gif\\\" style=\\\"vertical-align:middle\\\"/><em>C. laurentii</em> after 72 h cultivation. Meanwhile, CMC<img alt=\\\"single bond\\\" src=\\\"https://sdfestaticassets-us-east-1.sciencedirectassets.com/shared-assets/55/entities/sbnd.gif\\\" style=\\\"vertical-align:middle\\\"/><em>C. laurentii</em> reduced lesion diameter and disease incidence in fruit. This treatment promoted phenylpropane metabolism by activating PAL, C4H, 4CL, POD, and PPO and increasing the secondary metabolites. CMC<img alt=\\\"single bond\\\" src=\\\"https://sdfestaticassets-us-east-1.sciencedirectassets.com/shared-assets/55/entities/sbnd.gif\\\" style=\\\"vertical-align:middle\\\"/><em>C. laurentii</em> also activated the AsA-GSH cycle, enhanced the activities of SOD and CAT, and reduced the accumulation of H<sub>2</sub>O<sub>2</sub> and O<sub>2</sub><sup>•-</sup>. The results suggested that CMC<img alt=\\\"single bond\\\" src=\\\"https://sdfestaticassets-us-east-1.sciencedirectassets.com/shared-assets/55/entities/sbnd.gif\\\" style=\\\"vertical-align:middle\\\"/><em>C. laurentii</em> maintained high postharvest fruit quality in grapefruit fruit by elevating the phenylpropane and reactive oxygen metabolism.\",\"PeriodicalId\":8,\"journal\":{\"name\":\"ACS Biomaterials Science & Engineering\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2024-11-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Biomaterials Science & Engineering\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.foodchem.2024.141955\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Biomaterials Science & Engineering","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.foodchem.2024.141955","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Carboxymethyl cellulose-induced Cryptococcus laurentii improves disease resistance and regulates phenylpropane and reactive oxygen metabolism in grapefruit
Green mould disease poses a significant threat to the citrus industry. Cryptococcus laurentii can stimulate the fruit defence system, whereas the use of antagonistic yeast alone demonstrates limited efficacy. This study investigated the molecular mechanisms of C. laurentii cultured with carboxymethyl cellulose (CMCC. laurentii), and evaluated the effects of CMCC. laurentii on phenylpropane and reactive oxygen metabolism in grapefruit fruit. Transcriptome analysis revealed that the upregulation of gene expression associated with yeast growth and antagonistic ability occurred in CMCC. laurentii after 72 h cultivation. Meanwhile, CMCC. laurentii reduced lesion diameter and disease incidence in fruit. This treatment promoted phenylpropane metabolism by activating PAL, C4H, 4CL, POD, and PPO and increasing the secondary metabolites. CMCC. laurentii also activated the AsA-GSH cycle, enhanced the activities of SOD and CAT, and reduced the accumulation of H2O2 and O2•-. The results suggested that CMCC. laurentii maintained high postharvest fruit quality in grapefruit fruit by elevating the phenylpropane and reactive oxygen metabolism.
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C. laurentii), and evaluated the effects of CMC
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