Guobin Mao , Yuan Zeng , Chunmin Qiu , Guangmiao Ding , Leyao Li , Lixin Ma , Junbiao Dai , Wen Yin , Yingxin Ma
{"title":"用于监测高蛋白食品新鲜度的比率荧光纸芯片","authors":"Guobin Mao , Yuan Zeng , Chunmin Qiu , Guangmiao Ding , Leyao Li , Lixin Ma , Junbiao Dai , Wen Yin , Yingxin Ma","doi":"10.1016/j.aca.2024.343418","DOIUrl":null,"url":null,"abstract":"<div><div>Accurately monitoring the freshness of high-protein foods has significant implications for both food safety and public welfare. Since a large amount of hydrogen sulfide (H<sub>2</sub>S) is produced during spoilage-related processes, abnormal H<sub>2</sub>S levels are often considered an important indicator of food spoilage. Therefore, we synthesized novel nanoparticles (NPs) containing Silicon (Si) dots and CdTe quantum dots to accurately assess the amount of sulfide ions (S<sup>2−</sup>) and thus the quality of high-protein foods in the early stage of storage. As the concentration of S<sup>2−</sup> increased, the fluorescence intensity of Si/CdTe NPs at λ<sub>em</sub> = 488 nm increased, while the fluorescence intensity at λ<sub>em</sub> = 620 nm was quenched. The fluorescence intensity ratio (F<sub>620</sub>/F<sub>488</sub>) was negatively linearly correlated to S<sup>2−</sup> concentrations in the range of 1–20 μM, with a detection limit of 0.3 μM. Furthermore, to achieve portable detection, we mixed Si/CdTe NPs with sodium carboxymethyl cellulose to prepare effective visual fluorescent sensing paper chips, which exhibited ideal porous structure, good particle dispersion, and excellent fluorescence properties. Incubating the paper chips with high-protein foods allowed for accurate monitoring of food freshness during storage. Therefore, this approach provided a reliable and portable method to determine H<sub>2</sub>S concentration using a novel concept to ensure the freshness and safety of high-protein foods.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1334 ","pages":"Article 343418"},"PeriodicalIF":5.7000,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ratiometric fluorescent paper chip for monitoring the freshness of high protein foods\",\"authors\":\"Guobin Mao , Yuan Zeng , Chunmin Qiu , Guangmiao Ding , Leyao Li , Lixin Ma , Junbiao Dai , Wen Yin , Yingxin Ma\",\"doi\":\"10.1016/j.aca.2024.343418\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Accurately monitoring the freshness of high-protein foods has significant implications for both food safety and public welfare. Since a large amount of hydrogen sulfide (H<sub>2</sub>S) is produced during spoilage-related processes, abnormal H<sub>2</sub>S levels are often considered an important indicator of food spoilage. Therefore, we synthesized novel nanoparticles (NPs) containing Silicon (Si) dots and CdTe quantum dots to accurately assess the amount of sulfide ions (S<sup>2−</sup>) and thus the quality of high-protein foods in the early stage of storage. As the concentration of S<sup>2−</sup> increased, the fluorescence intensity of Si/CdTe NPs at λ<sub>em</sub> = 488 nm increased, while the fluorescence intensity at λ<sub>em</sub> = 620 nm was quenched. The fluorescence intensity ratio (F<sub>620</sub>/F<sub>488</sub>) was negatively linearly correlated to S<sup>2−</sup> concentrations in the range of 1–20 μM, with a detection limit of 0.3 μM. Furthermore, to achieve portable detection, we mixed Si/CdTe NPs with sodium carboxymethyl cellulose to prepare effective visual fluorescent sensing paper chips, which exhibited ideal porous structure, good particle dispersion, and excellent fluorescence properties. Incubating the paper chips with high-protein foods allowed for accurate monitoring of food freshness during storage. Therefore, this approach provided a reliable and portable method to determine H<sub>2</sub>S concentration using a novel concept to ensure the freshness and safety of high-protein foods.</div></div>\",\"PeriodicalId\":240,\"journal\":{\"name\":\"Analytica Chimica Acta\",\"volume\":\"1334 \",\"pages\":\"Article 343418\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2024-11-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytica Chimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0003267024012194\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytica Chimica Acta","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0003267024012194","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Ratiometric fluorescent paper chip for monitoring the freshness of high protein foods
Accurately monitoring the freshness of high-protein foods has significant implications for both food safety and public welfare. Since a large amount of hydrogen sulfide (H2S) is produced during spoilage-related processes, abnormal H2S levels are often considered an important indicator of food spoilage. Therefore, we synthesized novel nanoparticles (NPs) containing Silicon (Si) dots and CdTe quantum dots to accurately assess the amount of sulfide ions (S2−) and thus the quality of high-protein foods in the early stage of storage. As the concentration of S2− increased, the fluorescence intensity of Si/CdTe NPs at λem = 488 nm increased, while the fluorescence intensity at λem = 620 nm was quenched. The fluorescence intensity ratio (F620/F488) was negatively linearly correlated to S2− concentrations in the range of 1–20 μM, with a detection limit of 0.3 μM. Furthermore, to achieve portable detection, we mixed Si/CdTe NPs with sodium carboxymethyl cellulose to prepare effective visual fluorescent sensing paper chips, which exhibited ideal porous structure, good particle dispersion, and excellent fluorescence properties. Incubating the paper chips with high-protein foods allowed for accurate monitoring of food freshness during storage. Therefore, this approach provided a reliable and portable method to determine H2S concentration using a novel concept to ensure the freshness and safety of high-protein foods.
期刊介绍:
Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review.
Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.