Photochemical behavior of extracellular polymeric substances in intimately coupled TiO2 photocatalysis and biodegradation system

Photosensitization of extracellular polymeric substances (EPS) in aqueous environments is significant for pollutants degradation, but the synergistic effects in intimately coupled photocatalysis and biodegradation (ICPB) remain unknown. In this study, the pivotal role of EPS photosensitization in the degradation of 17β-estradiol 3-sulfate (E2-3S) was investigated in ICPB. Protein and polysaccharide contents in loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS) increased by 16.6, 9.15 and 9.2, 2.2 times compared with R1 (biofilm with light without photocatalyst) and R2 (biofilm with photocatalyst without light), respectively. During irradiation tests, more reactive species were generated in LB-EPS, and achieving 99.8 % degradation efficiency of E2-3S; tryptophan-like protein in EPS firstly to be converted, while the tyrosine-like protein underwent greater conversion; furthermore, hydrophilic molecules with O/C < 0.45 in EPS decreased and unsaturated molecules with H/C = 0.7–1.5 and O/C = 0–0.1 increased. This study reveals the photosensitization reaction of EPS in ICPB, which provides new insights for pollutants degradation.