分离、鉴定和优化利用香蕉皮废料生产酪氨酸酶的工业应用。

IF 1.9 3区 化学 Q3 CHEMISTRY, APPLIED Natural Product Research Pub Date : 2024-11-08 DOI:10.1080/14786419.2024.2426209
Raheela Jabeen, Ume Habiba, Tahmina Mustafa, Tayyeba Rehman
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引用次数: 0

摘要

该研究旨在从香蕉皮废料中分离、纯化和优化酪氨酸酶,以供工业使用。使用磷酸盐缓冲液从香蕉皮中提取酪氨酸酶。纯化首先是离心,然后进行硫酸铵沉淀。通过凝胶过滤进行进一步纯化和表征。布拉德福德测定法用于确定蛋白质浓度。使用酪氨酸酶活性测定法测量每个样品的酶活性。还对酪氨酸酶的 pH 值和温度进行了优化。结果表明,通过硫酸铵沉淀获得的 70% 饱和馏分比粗提取物和 35% 饱和馏分显示出更高的活性。凝胶过滤柱结果显示,馏分 17 和 18 的活性最高。酪氨酸酶在 pH 值为 7.0、温度为 37 ℃ 时活性最高。对工业纯化酶和实验室分离酶进行比较后发现,两者的最适 pH 值和温度相似。由此可以得出结论,香蕉皮可以作为合成酪氨酸酶的来源。
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Isolation, identification and optimization for tyrosinase production by banana peel waste for industrial application.

The study aimed to isolate, purify and optimise tyrosinase from banana peel waste for industrial use. Tyrosinase was extracted from banana peel using phosphate buffer. Purification initiated with centrifugation followed by ammonium sulphate precipitation. Further purification and characterisation was done through gel filtration. Bradford assay was used to determine the protein concentration. Enzyme activity of each sample was measured using tyrosinase activity assay. The pH and temperature optimisation of tyrosinase were also obtained. Results indicated that fractions obtained through ammonium sulphate precipitation at 70% saturation showed more activity than that of crude extract and 35% saturated fractions. Gel filtration column results showed that fraction number 17 &18 have maximum activity. Tyrosinase showed maximum activity at pH 7.0; 37 °C. Comparison between industrially purified and lab-isolated enzyme showed that both have similar optimum pH and temperature. It can be concluded that banana peel can be a source for synthesis of tyrosinase.

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来源期刊
Natural Product Research
Natural Product Research 化学-医药化学
CiteScore
5.10
自引率
9.10%
发文量
605
审稿时长
2.1 months
期刊介绍: The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.
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