Liguo Ji , Aoxiang Fu , Yuying Zhang, Ying Xu, Yanbei Xi, Shaoli Cui, Na Gao, Linlin Yang, Wanbing Shang, Zhijun Yang, Guangjie He
{"title":"AIE-TICT 荧光探针级联对 H2S、极性和粘度敏感，可跟踪缺血再灌注损伤细胞模型中的微环境变化","authors":"Liguo Ji , Aoxiang Fu , Yuying Zhang, Ying Xu, Yanbei Xi, Shaoli Cui, Na Gao, Linlin Yang, Wanbing Shang, Zhijun Yang, Guangjie He","doi":"10.1016/j.aca.2024.343425","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Ischemia-reperfusion injury is a common cause of cardiovascular and cerebrovascular diseases. The reoxygenation during reperfusion leads to an overproduction of reactive oxygen species (ROS). As an antioxidant, H<sub>2</sub>S can scavenge ROS to inhibit oxidative stress and inflammatory reaction, thus attenuating ischemia-reperfusion injury. In this process, the changes of cellular microenvironment (polarity or viscosity) have not been fully discussed. In order to real-time track the changes of cellular microenvironment during the treatment of ischemia-reperfusion injury with H<sub>2</sub>S. It is necessary to develop highly selective and sensitive probes that can cascade response to hydrogen sulfide and cellular microenvironment.</div></div><div><h3>Results</h3><div>We designed and synthesized a fluorescent probe TPEC-DNBS which can produce cascade response to H<sub>2</sub>S and microenvironment. An intermediate TPEC-OH is produced after highly selective and sensitive response to H<sub>2</sub>S, which can further respond to polarity and viscosity. In addition, due to the aggregation-induced emission (AIE) and twisted intramolecular charge transfer (TICT) effects, polarity can promote the fluorescence emission wavelength and intensity of TPEC-OH to produce double response characteristics, and its change trend (from weak green fluorescence at low polarity to strong red fluorescence at high polarity) is opposite to that of traditional polar probes (from strong green fluorescence at low polarity to weak red fluorescence at high polarity). Viscosity can only induce the change of fluorescence intensity. By constructing the cardiomyocyte model and hepatocyte model of ischemia-reperfusion, we further prove that after ischemia-reperfusion injury, the cells are in an environment of low polarity, and the microenvironment can be recovered after H<sub>2</sub>S treatment.</div></div><div><h3>Significance</h3><div>An AIE-TICT fluorescence probe capable of cascading responses to H<sub>2</sub>S, polarity and viscosity was constructed by using tetraphenylethylene and coumarin moieties. This probe provides a more intuitive and convenient condition for real-time tracking the changes of cellular microenvironment (polarity or viscosity) before and after H<sub>2</sub>S treatment of ischemia-reperfusion injury.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1334 ","pages":"Article 343425"},"PeriodicalIF":5.7000,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An AIE-TICT fluorescence probe cascade responsive to H2S, polarity and viscosity to track microenvironment changes in cellular model of ischemia-reperfusion injury\",\"authors\":\"Liguo Ji , Aoxiang Fu , Yuying Zhang, Ying Xu, Yanbei Xi, Shaoli Cui, Na Gao, Linlin Yang, Wanbing Shang, Zhijun Yang, Guangjie He\",\"doi\":\"10.1016/j.aca.2024.343425\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Ischemia-reperfusion injury is a common cause of cardiovascular and cerebrovascular diseases. The reoxygenation during reperfusion leads to an overproduction of reactive oxygen species (ROS). As an antioxidant, H<sub>2</sub>S can scavenge ROS to inhibit oxidative stress and inflammatory reaction, thus attenuating ischemia-reperfusion injury. In this process, the changes of cellular microenvironment (polarity or viscosity) have not been fully discussed. In order to real-time track the changes of cellular microenvironment during the treatment of ischemia-reperfusion injury with H<sub>2</sub>S. It is necessary to develop highly selective and sensitive probes that can cascade response to hydrogen sulfide and cellular microenvironment.</div></div><div><h3>Results</h3><div>We designed and synthesized a fluorescent probe TPEC-DNBS which can produce cascade response to H<sub>2</sub>S and microenvironment. An intermediate TPEC-OH is produced after highly selective and sensitive response to H<sub>2</sub>S, which can further respond to polarity and viscosity. In addition, due to the aggregation-induced emission (AIE) and twisted intramolecular charge transfer (TICT) effects, polarity can promote the fluorescence emission wavelength and intensity of TPEC-OH to produce double response characteristics, and its change trend (from weak green fluorescence at low polarity to strong red fluorescence at high polarity) is opposite to that of traditional polar probes (from strong green fluorescence at low polarity to weak red fluorescence at high polarity). Viscosity can only induce the change of fluorescence intensity. By constructing the cardiomyocyte model and hepatocyte model of ischemia-reperfusion, we further prove that after ischemia-reperfusion injury, the cells are in an environment of low polarity, and the microenvironment can be recovered after H<sub>2</sub>S treatment.</div></div><div><h3>Significance</h3><div>An AIE-TICT fluorescence probe capable of cascading responses to H<sub>2</sub>S, polarity and viscosity was constructed by using tetraphenylethylene and coumarin moieties. This probe provides a more intuitive and convenient condition for real-time tracking the changes of cellular microenvironment (polarity or viscosity) before and after H<sub>2</sub>S treatment of ischemia-reperfusion injury.</div></div>\",\"PeriodicalId\":240,\"journal\":{\"name\":\"Analytica Chimica Acta\",\"volume\":\"1334 \",\"pages\":\"Article 343425\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2024-11-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytica Chimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0003267024012261\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytica Chimica Acta","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0003267024012261","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
An AIE-TICT fluorescence probe cascade responsive to H2S, polarity and viscosity to track microenvironment changes in cellular model of ischemia-reperfusion injury
Background
Ischemia-reperfusion injury is a common cause of cardiovascular and cerebrovascular diseases. The reoxygenation during reperfusion leads to an overproduction of reactive oxygen species (ROS). As an antioxidant, H2S can scavenge ROS to inhibit oxidative stress and inflammatory reaction, thus attenuating ischemia-reperfusion injury. In this process, the changes of cellular microenvironment (polarity or viscosity) have not been fully discussed. In order to real-time track the changes of cellular microenvironment during the treatment of ischemia-reperfusion injury with H2S. It is necessary to develop highly selective and sensitive probes that can cascade response to hydrogen sulfide and cellular microenvironment.
Results
We designed and synthesized a fluorescent probe TPEC-DNBS which can produce cascade response to H2S and microenvironment. An intermediate TPEC-OH is produced after highly selective and sensitive response to H2S, which can further respond to polarity and viscosity. In addition, due to the aggregation-induced emission (AIE) and twisted intramolecular charge transfer (TICT) effects, polarity can promote the fluorescence emission wavelength and intensity of TPEC-OH to produce double response characteristics, and its change trend (from weak green fluorescence at low polarity to strong red fluorescence at high polarity) is opposite to that of traditional polar probes (from strong green fluorescence at low polarity to weak red fluorescence at high polarity). Viscosity can only induce the change of fluorescence intensity. By constructing the cardiomyocyte model and hepatocyte model of ischemia-reperfusion, we further prove that after ischemia-reperfusion injury, the cells are in an environment of low polarity, and the microenvironment can be recovered after H2S treatment.
Significance
An AIE-TICT fluorescence probe capable of cascading responses to H2S, polarity and viscosity was constructed by using tetraphenylethylene and coumarin moieties. This probe provides a more intuitive and convenient condition for real-time tracking the changes of cellular microenvironment (polarity or viscosity) before and after H2S treatment of ischemia-reperfusion injury.
期刊介绍：
Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review.
Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.
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