Xanthan gum production in Xanthomonas campestris is increased by favoring the biosynthesis of its monomers

Current efforts to improve xanthan gum (XG) production by Xanthomonas have focused on the growth medium, operating parameters, and downstream steps. However, a key aspect is the development of optimal strains. The present work aimed to investigate the formation of XG monomers, using kinetic and stoichiometric models to identify possible bottlenecks, and to engineer a recombinant strain to overcome such limitations. The galU and ugd genes involved in the biosynthesis of the UDP-glucose and UDP-glucuronic acid monomers were overexpressed in Xanthomonas campestris pv. campestris. The strains were cultivated in shake flasks and bioreactor. As predicted by in silico analysis, overexpression of the ugd gene resulted in a significant increase in gum synthesis, up to 50% higher volumetric productivity in the bioreactor. To a lesser extent, galU overexpression was also shown to improve product formation. These findings validated the hypothesis that metabolic engineering of the monomer biosynthesis can enhance XG production.