Qi Sun , Shiji Miao , Wenlong Yu , En-Yu Jiang , Mixue Gong , Genyan Liu , Xiaogang Luo , Ming-Zhi Zhang
{"title":"通过新型纤维素膜生物传感器的催化氧化作用，以肉眼可见的方式检测血清中的尿酸。","authors":"Qi Sun , Shiji Miao , Wenlong Yu , En-Yu Jiang , Mixue Gong , Genyan Liu , Xiaogang Luo , Ming-Zhi Zhang","doi":"10.1016/j.bios.2024.116912","DOIUrl":null,"url":null,"abstract":"<div><div>Uric acid (UA) serves as an important biochemical marker of various diseases, making the development of a novel method for its rapid and straightforward visual detection highly valuable. In this study, a uricase-based cellulose membrane biosensor (<strong>UCMB</strong>) was constructed by immobilizing uricase via a Schiff base reaction and nitroblue tetrazolium chloride (NBT) through adsorption. The <strong>UCMB</strong> detects UA through a mechanism in which uricase catalyzes the oxidation of UA, generation O<sub>2</sub><sup>−·</sup> radicals that subsequently oxidize NBT to formazan, producing a distinctive color change from yellow to purple. The <strong>UCMB</strong> demonstrated successful visual detection of UA within 15 min, allowing for rapid naked-eye analysis. Additionally, the biosensor quantitatively detected UA over a broad linear range from 0 to 1000 μM, with a low detection limit of 3.88 μM. Most notably, the <strong>UCMB</strong> has accurately measured UA in human serum samples, comparable to the results from a commercial UA meter. These findings suggest that the <strong>UCMB</strong> can serve as a simple and reliable tool for early diagnosis of UA-related diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"268 ","pages":"Article 116912"},"PeriodicalIF":10.7000,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Visual detection of uric acid in serum through catalytic oxidation by a novel cellulose membrane biosensor with schiff base immobilized uricase\",\"authors\":\"Qi Sun , Shiji Miao , Wenlong Yu , En-Yu Jiang , Mixue Gong , Genyan Liu , Xiaogang Luo , Ming-Zhi Zhang\",\"doi\":\"10.1016/j.bios.2024.116912\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Uric acid (UA) serves as an important biochemical marker of various diseases, making the development of a novel method for its rapid and straightforward visual detection highly valuable. In this study, a uricase-based cellulose membrane biosensor (<strong>UCMB</strong>) was constructed by immobilizing uricase via a Schiff base reaction and nitroblue tetrazolium chloride (NBT) through adsorption. The <strong>UCMB</strong> detects UA through a mechanism in which uricase catalyzes the oxidation of UA, generation O<sub>2</sub><sup>−·</sup> radicals that subsequently oxidize NBT to formazan, producing a distinctive color change from yellow to purple. The <strong>UCMB</strong> demonstrated successful visual detection of UA within 15 min, allowing for rapid naked-eye analysis. Additionally, the biosensor quantitatively detected UA over a broad linear range from 0 to 1000 μM, with a low detection limit of 3.88 μM. Most notably, the <strong>UCMB</strong> has accurately measured UA in human serum samples, comparable to the results from a commercial UA meter. These findings suggest that the <strong>UCMB</strong> can serve as a simple and reliable tool for early diagnosis of UA-related diseases.</div></div>\",\"PeriodicalId\":259,\"journal\":{\"name\":\"Biosensors and Bioelectronics\",\"volume\":\"268 \",\"pages\":\"Article 116912\"},\"PeriodicalIF\":10.7000,\"publicationDate\":\"2024-11-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biosensors and Bioelectronics\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0956566324009199\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956566324009199","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}
Visual detection of uric acid in serum through catalytic oxidation by a novel cellulose membrane biosensor with schiff base immobilized uricase
Uric acid (UA) serves as an important biochemical marker of various diseases, making the development of a novel method for its rapid and straightforward visual detection highly valuable. In this study, a uricase-based cellulose membrane biosensor (UCMB) was constructed by immobilizing uricase via a Schiff base reaction and nitroblue tetrazolium chloride (NBT) through adsorption. The UCMB detects UA through a mechanism in which uricase catalyzes the oxidation of UA, generation O2−· radicals that subsequently oxidize NBT to formazan, producing a distinctive color change from yellow to purple. The UCMB demonstrated successful visual detection of UA within 15 min, allowing for rapid naked-eye analysis. Additionally, the biosensor quantitatively detected UA over a broad linear range from 0 to 1000 μM, with a low detection limit of 3.88 μM. Most notably, the UCMB has accurately measured UA in human serum samples, comparable to the results from a commercial UA meter. These findings suggest that the UCMB can serve as a simple and reliable tool for early diagnosis of UA-related diseases.
期刊介绍：
Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.
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