揭示胸苷酸合成酶在人类癌症中的致癌意义。

IF 1.7 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL American journal of translational research Pub Date : 2024-10-15 eCollection Date: 2024-01-01 DOI:10.62347/IRUZ1011
Yibo Geng, Luyang Xie, Yang Wang, Yan Wang
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引用次数: 0

摘要

目的:胸苷酸合成酶(TYMS)是化疗耐药性的一个关键和有效靶点。然而,TYMS的致癌作用尚未得到足够的重视:利用癌症基因组图谱(TCGA)和各种公共数据库的数据,我们对 33 种癌症类型中 TYMS 的致癌作用进行了广泛调查。随后,我们在四种不同的细胞系中使用小干扰 RNA(siRNA)抑制了 TYMS,并使用 CellTiter-Glo 和 Transwell 检测法评估了细胞的增殖和迁移:结果:TYMS在多种癌症中均有明显表达,并在不同癌症患者群中显示出与临床结果的相关性。此外,在特定肿瘤类型中,基因改变被认为是影响总体生存的潜在因素。值得注意的是,在某些癌症中,胸苷酸合成酶的表达与肿瘤浸润的 CD4+ 细胞相关。此外,TYMS的功能机制还包括核苷酸酶活性、染色体分离和DNA复制进展。体外实验进一步证实了这些发现,表明抑制 TYMS 会阻碍 HeLa、A549、786-O 和 U87_MG 细胞的生长和侵袭能力:本研究全面揭示了 TYMS 在人类肿瘤中的致癌作用。
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Unveiling the oncogenic significance of thymidylate synthase in human cancers.

Objective: Thymidylate synthase (TYMS) constitutes a pivotal and potent target in the context of chemoresistance. However, the oncogenic role of TYMS has received insufficient attention.

Methods: Leveraging data from the Cancer Genome Atlas (TCGA) and various public databases, we conducted an extensive investigation into the oncogenic role of TYMS across 33 cancer types. Subsequently, TYMS was inhibited using small interfering RNA (siRNA) in four different cell lines, and cell proliferation and migration were assessed using CellTiter-Glo and Transwell assays.

Results: TYMS exhibited pronounced expression across a spectrum of cancers and demonstrated associations with clinical outcome in diverse cancer patient cohorts. Furthermore, genetic alterations were identified as potential influencers of overall survival in specific tumor types. Notably, the expression of thymidylate synthase correlated with tumor-infiltrating CD4+ cells in select cancers. Additionally, the functional mechanism of TYMS encompassed nucleotidase activity, chromosome segregation, and DNA replication progress. In vitro experiments further substantiated these findings, demonstrating that the suppression of TYMS impeded the cell growth and invasive capabilities of HeLa, A549, 786-O, and U87_MG cells.

Conclusions: This study furnishes a comprehensive understanding of the oncogenic role played by TYMS in human tumors.

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American journal of translational research
American journal of translational research ONCOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL
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