利用三元载体系统和形态发生调节剂高效转化高粱和玉米。

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-11-11 DOI:10.1111/tpj.17101
Juan B Fontanet-Manzaneque, Jari Haeghebaert, Stijn Aesaert, Griet Coussens, Laurens Pauwels, Ana I Caño-Delgado
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引用次数: 0

摘要

高粱(Sorghum bicolor)是一种重要的 C4 单子叶作物,栽培于世界各地的干旱地区,对人类和动物的营养都有重要价值。尽管高粱在农业上占有重要地位,但其研究却因转化频率低而受到阻碍。在本研究中,我们使用农杆菌的 pVS1-VIR2 三元载体系统,结合形态发生基因 BABY BOOM 和 WUSCHEL2,并使用 G418 进行筛选,对高粱的转化进行了研究。我们针对细菌光密度、共培养时间和温度等关键参数,优化了农杆菌介导的感染。此外,基于切除的转化系统使我们能够生成不含形态发生调节因子的转基因植物。这种方法的转化率非常高,根据分离的小植株总数计算,转化率高达 164.8%。同样的三元载体、形态发生基因和基于遗传因子的选择组合也显著提高了玉米近交系 B104 的转化效率。利用这种方法进行基因组编辑的潜力使其成为开发符合不断发展的欧洲法规的高粱和玉米品种的重要工具。我们的工作标志着高粱生物技术取得了重大进展,有望在不断变化的气候条件下应对全球粮食安全挑战。
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Efficient sorghum and maize transformation using a ternary vector system combined with morphogenic regulators.

Sorghum bicolor (sorghum) is a vital C4 monocotyledon crop cultivated in arid regions worldwide, valued for its significance in both human and animal nutrition. Despite its agricultural prominence, sorghum research has been hindered by low transformation frequency. In this study, we examined sorghum transformation using the pVS1-VIR2 ternary vector system for Agrobacterium, combined with the morphogenic genes BABY BOOM and WUSCHEL2 and selection using G418. We optimized Agrobacterium-mediated infection, targeting key parameters such as bacterial optical density, co-cultivation time, and temperature. Additionally, an excision-based transformation system enabled us to generate transgenic plants free of morphogenic regulators. The method yielded remarkable transformation frequencies, reaching up to 164.8% based on total isolated plantlets. The same combination of ternary vector, morphogenic genes and geneticin-based selection also resulted in a marked increase in transformation efficiency of the Zea mays (maize) inbred line B104. The potential for genomic editing using this approach positions it as a valuable tool for the development of sorghum and maize varieties that comply with evolving European regulations. Our work marks a significant stride in sorghum biotechnology and holds promise for addressing global food security challenges in a changing climate.

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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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