以单碱基分辨率高通量检测 RNA 修饰。

IF 3.5 4区生物学 Q1 Biochemistry, Genetics and Molecular Biology FEBS Letters Pub Date : 2024-11-14 DOI:10.1002/1873-3468.15052

Keren Ron, Joshua Kahn, Nofar Malka-Tunitsky, Aldema Sas-Chen

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引用次数: 0

摘要

RNA 可通过 > 170 种化学修饰进行修饰，从而影响其结构和功能。因此，RNA 修饰与跨越系统发育树的各种物种的基因表达和细胞结果的调控有关。以单碱基分辨率检测 RNA 修饰的高通量方法的产生加速了对 RNA 修饰的研究。在此，我们回顾了基于新一代测序方法检测 rRNA、tRNA 和 mRNA 中 14 种不同 RNA 修饰的最新进展。我们进一步概述了目前可用方法的分子和计算原理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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High-throughput detection of RNA modifications at single base resolution.

RNA is modified by > 170 chemical modifications that affect its structure and function. Accordingly, RNA modifications have been implicated in regulation of gene expression and cellular outcomes in a variety of species spanning the phylogenetic tree. The study of RNA modifications is accelerated by generation of high-throughput methods for detecting RNA modifications at single base resolution. Here, we review recent advancement in next generation sequencing based approaches for detection of 14 distinct RNA modifications present in rRNA, tRNA and mRNA. We further outline the molecular and computational principles underlying currently available methods.

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来源期刊

FEBS Letters 生物-生化与分子生物学

CiteScore

7.00

自引率

2.90%

发文量

303

审稿时长

1.0 months

期刊介绍： FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.