W A Saka, O S Oyekunle, T M Akhigbe, O O Oladipo, M B Ajayi, A T Adekola, A I Omole, R E Akhigbe
{"title":"穿心莲通过提高 Wistar 大鼠的胰岛素敏感性和上调 GLUT 4 的表达来改善葡萄糖调节。","authors":"W A Saka, O S Oyekunle, T M Akhigbe, O O Oladipo, M B Ajayi, A T Adekola, A I Omole, R E Akhigbe","doi":"10.3389/fnut.2024.1416641","DOIUrl":null,"url":null,"abstract":"<p><strong>Context: </strong>Although the hypoglycaemic effect of <i>Andrographis paniculata (Burm.f.) Nees [Acanthaceae]</i> has been documented, reports on its effect in an apparently healthy state are limited.</p><p><strong>Objective: </strong>This study investigated whether or not <i>A. paniculata</i> exerts hypoglycaemic effect in a non-diabetic state. It also explored the impact of <i>A. paniculata</i> on glycolytic enzymes and GLUT 4 protein expression, as a possible mode of action.</p><p><strong>Methods: </strong>Twenty male Wistar rats were randomly assigned into two groups (<i>n</i> = 10 rats/group). The control rats were vehicle-treated (0.5 ml of distilled water), while the <i>A. paniculata</i>-treated rats had 500 mg/kg of <i>A. paniculata per os</i> once daily for 35 days.</p><p><strong>Results: </strong><i>A. paniculata</i> treatment led to improved insulin sensitivity evidenced by increased HOMA-β (88.08 ± 2.13 vs. 120.80 ± 1.52, <i>p</i> < 0.0001), HOMA-S (283.60 ± 8.82 vs. 300.50 ± 9.30, <i>p</i> = 0.0189), and reduced TyG index (4.22 ± 0.04 vs. 3.95 ± 0.07, <i>p</i> < 0.0002) and HOMA-IR (0.32 ± 0.01 vs. 0.25 ± 0.01, <i>p</i> < 0.0001) when compared with the control. It also improved glucose regulation as depicted by reduced fasting blood glucose (3.77 ± 0.10 vs. 3.24 ± 0.11, <i>p</i> < 0.0001) and glycated hemoglobin (HbA1c; 7.69 ± 1.15 vs. 5.95 ± 0.82, <i>p</i> = 0.0245), and atherogenic dyslipidaemia, including AIP (-0.12 ± 0.03 vs. -0.26 ± 0.03, <i>p</i> < 0.0001) and CRI-I (2.70 ± 0.29 vs. 1.84 ± 0.27, <i>p</i> < 0.0001). These findings were accompanied by enhanced hepatic and muscular redox state, increased activities of glycolytic enzymes, upregulated GLUT 4 (0.80 ± 0.27 vs. 6.20 ± 0.84, <i>p</i> < 0.0001), and increased circulating nitric oxide (5.45 ± 0.24 vs. 6.79 ± 0.33, <i>p</i> = 0.0002).</p><p><strong>Conclusion: </strong><i>A. paniculata</i> exerts positive effect on glucose metabolism and utilization by improving insulin sensitivity and upregulating the activities of glycolytic enzymes and GLUT 4 protein expression. This implies that <i>A. paniculata</i> may be beneficial in preventing insulin resistance and incident diabetes. Nonetheless, it should be used with caution to prevent hypoglycaemia in a non-diabetic state.</p>","PeriodicalId":12473,"journal":{"name":"Frontiers in Nutrition","volume":"11 ","pages":"1416641"},"PeriodicalIF":4.0000,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11562748/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>Andrographis paniculata</i> improves glucose regulation by enhancing insulin sensitivity and upregulating GLUT 4 expression in Wistar rats.\",\"authors\":\"W A Saka, O S Oyekunle, T M Akhigbe, O O Oladipo, M B Ajayi, A T Adekola, A I Omole, R E Akhigbe\",\"doi\":\"10.3389/fnut.2024.1416641\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Context: </strong>Although the hypoglycaemic effect of <i>Andrographis paniculata (Burm.f.) Nees [Acanthaceae]</i> has been documented, reports on its effect in an apparently healthy state are limited.</p><p><strong>Objective: </strong>This study investigated whether or not <i>A. paniculata</i> exerts hypoglycaemic effect in a non-diabetic state. It also explored the impact of <i>A. paniculata</i> on glycolytic enzymes and GLUT 4 protein expression, as a possible mode of action.</p><p><strong>Methods: </strong>Twenty male Wistar rats were randomly assigned into two groups (<i>n</i> = 10 rats/group). The control rats were vehicle-treated (0.5 ml of distilled water), while the <i>A. paniculata</i>-treated rats had 500 mg/kg of <i>A. paniculata per os</i> once daily for 35 days.</p><p><strong>Results: </strong><i>A. paniculata</i> treatment led to improved insulin sensitivity evidenced by increased HOMA-β (88.08 ± 2.13 vs. 120.80 ± 1.52, <i>p</i> < 0.0001), HOMA-S (283.60 ± 8.82 vs. 300.50 ± 9.30, <i>p</i> = 0.0189), and reduced TyG index (4.22 ± 0.04 vs. 3.95 ± 0.07, <i>p</i> < 0.0002) and HOMA-IR (0.32 ± 0.01 vs. 0.25 ± 0.01, <i>p</i> < 0.0001) when compared with the control. It also improved glucose regulation as depicted by reduced fasting blood glucose (3.77 ± 0.10 vs. 3.24 ± 0.11, <i>p</i> < 0.0001) and glycated hemoglobin (HbA1c; 7.69 ± 1.15 vs. 5.95 ± 0.82, <i>p</i> = 0.0245), and atherogenic dyslipidaemia, including AIP (-0.12 ± 0.03 vs. -0.26 ± 0.03, <i>p</i> < 0.0001) and CRI-I (2.70 ± 0.29 vs. 1.84 ± 0.27, <i>p</i> < 0.0001). These findings were accompanied by enhanced hepatic and muscular redox state, increased activities of glycolytic enzymes, upregulated GLUT 4 (0.80 ± 0.27 vs. 6.20 ± 0.84, <i>p</i> < 0.0001), and increased circulating nitric oxide (5.45 ± 0.24 vs. 6.79 ± 0.33, <i>p</i> = 0.0002).</p><p><strong>Conclusion: </strong><i>A. paniculata</i> exerts positive effect on glucose metabolism and utilization by improving insulin sensitivity and upregulating the activities of glycolytic enzymes and GLUT 4 protein expression. This implies that <i>A. paniculata</i> may be beneficial in preventing insulin resistance and incident diabetes. Nonetheless, it should be used with caution to prevent hypoglycaemia in a non-diabetic state.</p>\",\"PeriodicalId\":12473,\"journal\":{\"name\":\"Frontiers in Nutrition\",\"volume\":\"11 \",\"pages\":\"1416641\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-10-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11562748/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Nutrition\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3389/fnut.2024.1416641\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Nutrition","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3389/fnut.2024.1416641","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
引用次数: 0
摘要
背景：虽然穿心莲（Andrographis paniculata (Burm.f.) Nees [Acanthaceae]）有降血糖作用的记载，但有关其在明显健康状态下降血糖作用的报道却很有限：本研究调查了穿心莲在非糖尿病状态下是否具有降血糖作用。本研究还探讨了 A. paniculata 对糖酵解酶和 GLUT 4 蛋白表达的影响，以此作为一种可能的作用模式：将 20 只雄性 Wistar 大鼠随机分为两组（n = 10 只/组）。对照组大鼠接受药物治疗（0.5 毫升蒸馏水），而西洋鹅掌楸治疗组大鼠每只口服 500 毫克/千克西洋鹅掌楸，每天一次，连续 35 天：结果：茨菰治疗可改善胰岛素敏感性，表现为 HOMA-β 增加（88.08 ± 2.13 vs. 120.80 ± 1.52，p p = 0.0189）、TyG 指数降低（4.22 ± 0.04 vs. 3.95 ± 0.07，p p p = 0.0245）和致动脉粥样硬化性血脂异常，包括 AIP（-0.12 ± 0.03 vs. -0.26 ± 0.03，p p p = 0.0002）：结论：白花蛇舌草通过改善胰岛素敏感性、上调糖酵解酶活性和 GLUT 4 蛋白表达，对葡萄糖代谢和利用产生积极影响。这意味着A. paniculata对预防胰岛素抵抗和糖尿病可能有益。不过，在非糖尿病状态下，应谨慎使用它来预防低血糖症。
Andrographis paniculata improves glucose regulation by enhancing insulin sensitivity and upregulating GLUT 4 expression in Wistar rats.
Context: Although the hypoglycaemic effect of Andrographis paniculata (Burm.f.) Nees [Acanthaceae] has been documented, reports on its effect in an apparently healthy state are limited.
Objective: This study investigated whether or not A. paniculata exerts hypoglycaemic effect in a non-diabetic state. It also explored the impact of A. paniculata on glycolytic enzymes and GLUT 4 protein expression, as a possible mode of action.
Methods: Twenty male Wistar rats were randomly assigned into two groups (n = 10 rats/group). The control rats were vehicle-treated (0.5 ml of distilled water), while the A. paniculata-treated rats had 500 mg/kg of A. paniculata per os once daily for 35 days.
Results: A. paniculata treatment led to improved insulin sensitivity evidenced by increased HOMA-β (88.08 ± 2.13 vs. 120.80 ± 1.52, p < 0.0001), HOMA-S (283.60 ± 8.82 vs. 300.50 ± 9.30, p = 0.0189), and reduced TyG index (4.22 ± 0.04 vs. 3.95 ± 0.07, p < 0.0002) and HOMA-IR (0.32 ± 0.01 vs. 0.25 ± 0.01, p < 0.0001) when compared with the control. It also improved glucose regulation as depicted by reduced fasting blood glucose (3.77 ± 0.10 vs. 3.24 ± 0.11, p < 0.0001) and glycated hemoglobin (HbA1c; 7.69 ± 1.15 vs. 5.95 ± 0.82, p = 0.0245), and atherogenic dyslipidaemia, including AIP (-0.12 ± 0.03 vs. -0.26 ± 0.03, p < 0.0001) and CRI-I (2.70 ± 0.29 vs. 1.84 ± 0.27, p < 0.0001). These findings were accompanied by enhanced hepatic and muscular redox state, increased activities of glycolytic enzymes, upregulated GLUT 4 (0.80 ± 0.27 vs. 6.20 ± 0.84, p < 0.0001), and increased circulating nitric oxide (5.45 ± 0.24 vs. 6.79 ± 0.33, p = 0.0002).
Conclusion: A. paniculata exerts positive effect on glucose metabolism and utilization by improving insulin sensitivity and upregulating the activities of glycolytic enzymes and GLUT 4 protein expression. This implies that A. paniculata may be beneficial in preventing insulin resistance and incident diabetes. Nonetheless, it should be used with caution to prevent hypoglycaemia in a non-diabetic state.
期刊介绍：
No subject pertains more to human life than nutrition. The aim of Frontiers in Nutrition is to integrate major scientific disciplines in this vast field in order to address the most relevant and pertinent questions and developments. Our ambition is to create an integrated podium based on original research, clinical trials, and contemporary reviews to build a reputable knowledge forum in the domains of human health, dietary behaviors, agronomy & 21st century food science. Through the recognized open-access Frontiers platform we welcome manuscripts to our dedicated sections relating to different areas in the field of nutrition with a focus on human health.
Specialty sections in Frontiers in Nutrition include, for example, Clinical Nutrition, Nutrition & Sustainable Diets, Nutrition and Food Science Technology, Nutrition Methodology, Sport & Exercise Nutrition, Food Chemistry, and Nutritional Immunology. Based on the publication of rigorous scientific research, we thrive to achieve a visible impact on the global nutrition agenda addressing the grand challenges of our time, including obesity, malnutrition, hunger, food waste, sustainability and consumer health.
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