{"title":"利用 HPLC-ELSD 和 HPLC-MS 对瘘管属精液中的甾体皂苷进行定量测定","authors":"Zhancai Zheng, Deduo Xu, Wenquan Lu, Wansheng Chen, Yingbo Yang, Zhijun Wu","doi":"10.1155/2024/1872918","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, high-performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) and HPLC-mass spectrometry (HPLC-MS) were employed for the quantitative analysis of steroidal saponins of <i>Semen Allii Fistulosi</i>. The HPLC-ELSD method was simple, accurate, and repeatable, and the recoveries were 97.91%∼99.77%. Similarly, the HPLC-MS method was rapid, sensitive, accurate, and reproducible, and the recoveries ranging from 95.61% to 99.70% in the control samples. The sensitivity of the HPLC-MS method was several times higher than that of the HPLC-ELSD method. These results collectively demonstrated that these methods are suitable for the content determination of steroidal saponins of <i>Semen Allii Fistulosi</i> and the identification of its plant source.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2024 ","pages":"1872918"},"PeriodicalIF":2.3000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11548948/pdf/","citationCount":"0","resultStr":"{\"title\":\"Quantitative Determination of Steroidal Saponins of <i>Semen Allii Fistulosi</i> Using HPLC-ELSD and HPLC-MS.\",\"authors\":\"Zhancai Zheng, Deduo Xu, Wenquan Lu, Wansheng Chen, Yingbo Yang, Zhijun Wu\",\"doi\":\"10.1155/2024/1872918\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In this study, high-performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) and HPLC-mass spectrometry (HPLC-MS) were employed for the quantitative analysis of steroidal saponins of <i>Semen Allii Fistulosi</i>. The HPLC-ELSD method was simple, accurate, and repeatable, and the recoveries were 97.91%∼99.77%. Similarly, the HPLC-MS method was rapid, sensitive, accurate, and reproducible, and the recoveries ranging from 95.61% to 99.70% in the control samples. The sensitivity of the HPLC-MS method was several times higher than that of the HPLC-ELSD method. These results collectively demonstrated that these methods are suitable for the content determination of steroidal saponins of <i>Semen Allii Fistulosi</i> and the identification of its plant source.</p>\",\"PeriodicalId\":14974,\"journal\":{\"name\":\"Journal of Analytical Methods in Chemistry\",\"volume\":\"2024 \",\"pages\":\"1872918\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11548948/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Analytical Methods in Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1155/2024/1872918\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Analytical Methods in Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1155/2024/1872918","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Quantitative Determination of Steroidal Saponins of Semen Allii Fistulosi Using HPLC-ELSD and HPLC-MS.
In this study, high-performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) and HPLC-mass spectrometry (HPLC-MS) were employed for the quantitative analysis of steroidal saponins of Semen Allii Fistulosi. The HPLC-ELSD method was simple, accurate, and repeatable, and the recoveries were 97.91%∼99.77%. Similarly, the HPLC-MS method was rapid, sensitive, accurate, and reproducible, and the recoveries ranging from 95.61% to 99.70% in the control samples. The sensitivity of the HPLC-MS method was several times higher than that of the HPLC-ELSD method. These results collectively demonstrated that these methods are suitable for the content determination of steroidal saponins of Semen Allii Fistulosi and the identification of its plant source.
期刊介绍：
Journal of Analytical Methods in Chemistry publishes papers reporting methods and instrumentation for chemical analysis, and their application to real-world problems. Articles may be either practical or theoretical.
Subject areas include (but are by no means limited to):
Separation
Spectroscopy
Mass spectrometry
Chromatography
Analytical Sample Preparation
Electrochemical analysis
Hyphenated techniques
Data processing
As well as original research, Journal of Analytical Methods in Chemistry also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.
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