5-氟尿嘧啶负载壳聚糖纳米粒子对 A375 和 A431 细胞系治疗皮肤癌的影响

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY Tissue & cell Pub Date : 2024-11-04 DOI:10.1016/j.tice.2024.102606

Bindu Kumari Yadav , Riya Manoj Kataria

{"title":"5-氟尿嘧啶负载壳聚糖纳米粒子对 A375 和 A431 细胞系治疗皮肤癌的影响","authors":"Bindu Kumari Yadav , Riya Manoj Kataria","doi":"10.1016/j.tice.2024.102606","DOIUrl":null,"url":null,"abstract":"<div><div>Skin cancer is an uncontrolled proliferation of abnormal skin cells that is usually caused by damaging UV radiation. Skin cancer is classified into two types: non-melanoma skin cancer and melanoma skin cancer. The entire studies focus on cytotoxic effect of prepared 5-Flurouracil loaded chitosan nanoparticle using A375 and A431 skin cancer cell line. <em>In-vitro</em> cell cytotoxicity assay, DAPI test of prepared formulation was conducted to observe the cytotoxic effect. The cell cytotoxicity investigation revealed that nanoparticles had higher cytotoxicity, inducing greater apoptosis within 72 h. The experimental results show that the produced nanoparticle is a good candidate for use as a 5-FU carrier in the treatment of skin cancer.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"91 ","pages":"Article 102606"},"PeriodicalIF":2.7000,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Impact of 5-Flurouracil loaded chitosan nanoparticle on A375 and A431 cell line for the therapy of skin cancer\",\"authors\":\"Bindu Kumari Yadav , Riya Manoj Kataria\",\"doi\":\"10.1016/j.tice.2024.102606\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Skin cancer is an uncontrolled proliferation of abnormal skin cells that is usually caused by damaging UV radiation. Skin cancer is classified into two types: non-melanoma skin cancer and melanoma skin cancer. The entire studies focus on cytotoxic effect of prepared 5-Flurouracil loaded chitosan nanoparticle using A375 and A431 skin cancer cell line. <em>In-vitro</em> cell cytotoxicity assay, DAPI test of prepared formulation was conducted to observe the cytotoxic effect. The cell cytotoxicity investigation revealed that nanoparticles had higher cytotoxicity, inducing greater apoptosis within 72 h. The experimental results show that the produced nanoparticle is a good candidate for use as a 5-FU carrier in the treatment of skin cancer.</div></div>\",\"PeriodicalId\":23201,\"journal\":{\"name\":\"Tissue & cell\",\"volume\":\"91 \",\"pages\":\"Article 102606\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-11-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tissue & cell\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0040816624003070\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ANATOMY & MORPHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tissue & cell","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0040816624003070","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

皮肤癌是一种不受控制的异常皮肤细胞增生，通常由有害的紫外线辐射引起。皮肤癌分为两种类型：非黑色素瘤皮肤癌和黑色素瘤皮肤癌。整个研究的重点是利用 A375 和 A431 皮肤癌细胞系，研究制备的负载 5-氟尿嘧啶的壳聚糖纳米粒子的细胞毒性效应。为观察细胞毒性效果，对制备的制剂进行了体外细胞毒性试验和 DAPI 试验。细胞毒性研究表明，纳米颗粒具有较高的细胞毒性，可在 72 小时内诱导细胞凋亡。实验结果表明，所制备的纳米颗粒可用作治疗皮肤癌的 5-FU 载体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Impact of 5-Flurouracil loaded chitosan nanoparticle on A375 and A431 cell line for the therapy of skin cancer

Skin cancer is an uncontrolled proliferation of abnormal skin cells that is usually caused by damaging UV radiation. Skin cancer is classified into two types: non-melanoma skin cancer and melanoma skin cancer. The entire studies focus on cytotoxic effect of prepared 5-Flurouracil loaded chitosan nanoparticle using A375 and A431 skin cancer cell line. In-vitro cell cytotoxicity assay, DAPI test of prepared formulation was conducted to observe the cytotoxic effect. The cell cytotoxicity investigation revealed that nanoparticles had higher cytotoxicity, inducing greater apoptosis within 72 h. The experimental results show that the produced nanoparticle is a good candidate for use as a 5-FU carrier in the treatment of skin cancer.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.