S100A1 过表达可刺激细胞增殖,并可预测卵巢癌的不良预后。

IF 1.5 4区 医学 Q4 ONCOLOGY Translational cancer research Pub Date : 2024-10-31 Epub Date: 2024-10-21 DOI:10.21037/tcr-24-430
Wen Jin, Hui Hui, Jie Jiang, Bin Li, Zhuo Deng, Xiaoqian Tuo
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引用次数: 0

摘要

背景:在包括卵巢癌(OC)在内的各种癌症中,S100 基因家族的成员经常发生调控失调。尽管如此,人们对单个S100基因在卵巢癌中的预后影响仍知之甚少。本研究旨在探讨S100A1表达在OC中的预后意义,并评估其作为治疗靶点的潜力:为了研究S100A1在OC中的作用,我们利用了基因表达谱交互分析(GEPIA)数据库和伯明翰阿拉巴马大学癌症数据分析门户(UALCAN)数据库。采用免疫印迹法和免疫组化法评估了OC组织中S100A1的蛋白水平。生物信息学分析将 S100A1 的表达与临床结果相关联。我们进行了功能测试,以评估 S100A1 基因敲除对 OC 细胞增殖和迁移的影响。此外,我们还研究了S100A1对肿瘤细胞中铁细胞凋亡和脂质活性氧(ROS)水平的影响:结果:我们的分析发现,与正常组织相比,OC 组织中 S100A1 蛋白水平明显升高。S100A1表达的升高与OC患者的不良临床预后有关。功能测试表明,敲除 S100A1 会导致体外 OC 细胞的增殖和迁移减少。此外,研究还发现S100A1能抑制OC细胞的铁突变,从而降低肿瘤细胞内的脂质ROS水平:结论:高水平的S100A1是OC不良临床结局的标志。我们的研究结果表明,S100A1可作为有价值的预后标志物和OC治疗的潜在治疗靶点。
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S100A1 overexpression stimulates cell proliferation and is predictive of poor outcome in ovarian cancer.

Background: Members of the S100 gene family are frequently dysregulated in various cancers, including ovarian cancer (OC). Despite this, the prognostic implications of individual S100 genes in OC remain poorly understood. This study aimed to explore the prognostic significance of S100A1 expression in OC and assess its potential as a therapeutic target.

Methods: To investigate the role of S100A1 in OC, we utilized the Gene Expression Profiling Interactive Analysis (GEPIA) database and the University of ALabama at Birmingham Cancer Data Analysis Portal (UALCAN) database. Protein levels of S100A1 in OC tissues were assessed using western blotting and immunohistochemistry. Bioinformatics analyses were performed to correlate S100A1 expression with clinical outcomes. Functional assays were conducted to evaluate the impact of S100A1 knockout on OC cell proliferation and migration. Additionally, we investigated the effect of S100A1 on ferroptosis and lipid reactive oxygen species (ROS) levels in tumor cells.

Results: Our analyses revealed that S100A1 protein levels were significantly elevated in OC tissues compared to normal tissues. Elevated S100A1 expression was associated with poor clinical outcomes in OC patients. Functional assays demonstrated that the knockout of S100A1 led to a decrease in both proliferation and migration of OC cells in vitro. Furthermore, S100A1 was found to inhibit ferroptosis in OC cells, resulting in lower levels of lipid ROS within tumor cells.

Conclusions: High levels of S100A1 are indicative of adverse clinical outcomes in OC. Our findings suggest that S100A1 could serve as a valuable prognostic marker and a potential therapeutic target for OC treatment.

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来源期刊
CiteScore
2.10
自引率
0.00%
发文量
252
期刊介绍: Translational Cancer Research (Transl Cancer Res TCR; Print ISSN: 2218-676X; Online ISSN 2219-6803; http://tcr.amegroups.com/) is an Open Access, peer-reviewed journal, indexed in Science Citation Index Expanded (SCIE). TCR publishes laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer; results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of cancer patients. The focus of TCR is original, peer-reviewed, science-based research that successfully advances clinical medicine toward the goal of improving patients'' quality of life. The editors and an international advisory group of scientists and clinician-scientists as well as other experts will hold TCR articles to the high-quality standards. We accept Original Articles as well as Review Articles, Editorials and Brief Articles.
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