Response Surface Optimization of Solvent-Tolerant Cold-Active Lipase Production by Pseudomonas sp. VITCLP4

The yield of solvent-tolerant cold-active lipase from halo-tolerant Pseudomonas sp. VITCLP4 was improved by applying statistical methods to precisely combine medium components and other conditions. A two-level Plackett–Burman (PB) design was utilized to screen the most significant variables from eleven variables that influence lipase production. Analysis of variance revealed that Tween-80, peptone, and KH₂PO₄ were statistically significant. Optimum levels of selected variables were ascertained by one-factor-at-a-time (OFAT) analysis. The selected three components' optimum values and interactive effects were established by response surface methodology (RSM) using a five-level-three-factor approach based on the central composite design (CCD). The optimized medium containing Tween-80, 0.6% (v/v), peptone, 0.85% (w/v), KH₂PO₄, 0.1% (w/v), yeast extract, 0.1% (w/v), olive oil, 0.2% (v/v) and inoculum size, 0.2% (v/v) resulted in maximum lipase production of 1356 Units mL⁻¹ min⁻¹ with 7.9-fold increase in the yield. This study provides insights into enhancing lipase production statistically with optimized resources that can be utilized in bioprocess studies and industrial applications.

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