Spectrum-effect relationship between HPLC fingerprints and antioxidant activities of Bletilla striata

Bletilla striata is a perennial herb that was first published in Shennong’s Classic of the Materia Medica, pharmacological studies have shown that it has the activities of promoting wound healing, anti-inflammatory and antioxidant. However, the relationship between the antioxidant activity and the chemical composition of Bletilla striata is still unclear. In this paper, the chemometric method was used to construct the spectral effect relationship between the fingerprints of 20 batches of Bletilla striata extracts from different origins and their in vitro antioxidant activities. The results showed that the chemical composition of the samples from different sources varied significantly, while the samples from Shaanxi and Hubei provinces were of relatively better quality. Among the 10 common peaks, coelonin, gymnoside IX and dactylorhin A were considered to be significantly correlated with the antioxidant activity of Bletilla striata. The results of this study will provide a basis and further insights for the quality evaluation and quality control of Bletilla striata.