动态转录组揭示烟草(Nicotiana tabacum L.)对黑柄病的反应模式

IF 6.8 Q1 PLANT SCIENCES Plant Stress Pub Date : 2024-11-14 DOI:10.1016/j.stress.2024.100676
Zhijun Tong , Zuoqian Fan , Tianyu Du , Dunhuang Fang , Xueyi Sui , Chuyu Ye , Qian-Hao Zhu , Longjiang Fan , Bingguang Xiao , Enhui Shen
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引用次数: 0

摘要

烟草黑柄病由烟草疫霉菌(Phytophthora nicotianae)引起,是烟草生产中产量损失的主要原因之一。本研究旨在探索对黑柄病具有抗性或敏感性的烟草基因型的动态转录组,并了解烟草对烟粉虱感染的防御反应。研究人员在感染后 0、12、24、48 和 72 小时分别对两种抗病材料和两种易感材料的根部和茎部进行了取样,并对其进行了 RNA 测序。传统的识别差异表达基因的方法并不是处理复杂数据集的最佳方法,因此我们采用了一种计算样本间标准偏差的新方法来识别四种材料的根和茎在不同时间点的不同表达水平的转录本。在总共 229,501 个转录本中,发现了 7,261 个可变转录本,其中许多被注释为与病原体感染的防御反应有关。这些可变转录本的表达模式在根和茎之间以及抗性材料和易感材料之间存在显著差异。有几个转录本被确定为进一步功能表征的潜在候选转录本。我们的研究结果为我们深入了解烟草对烟粉虱感染的防御反应中的动态转录组提供了宝贵的信息。
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The dynamic transcriptome reveals response patterns to black shank disease in tobacco (Nicotiana tabacum L.)
Black shank disease, caused by Phytophthora nicotianae, is one of the major causes of yield loss in tobacco production. The present study aimed to explore the dynamic transcriptome in tobacco genotypes resistant or susceptible to black shank disease and to understand the defense response of tobacco to P. nicotianae infection. Roots and stems were sampled from two resistant and two susceptible materials at 0, 12, 24, 48, and 72 h post infection and used in RNA-sequencing. Conventional approaches that identify differentially expressed genes are not the best way for handling the complex datasets, so a new method that calculates the standard deviation among samples was applied to identify transcripts with variable expression levels in roots and stems of the four materials at different time points. Of the total of 229,501 transcripts, 7,261 were found to be variable transcripts, with many of them annotated to be related to defense responses against pathogen infection. These variable transcripts showed expression patterns that varied significantly between roots and stems as well as between the resistant and the susceptible materials. Several transcripts were identified to be potential candidates for further functional characterization. Our findings provide invaluable insights into the dynamic transcriptome in defense responses of tobacco against P. nicotianae infection.
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来源期刊
Plant Stress
Plant Stress PLANT SCIENCES-
CiteScore
5.20
自引率
8.00%
发文量
76
审稿时长
63 days
期刊最新文献
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