Zhaleh Asadi Fakhr , Xinlan Zhu , Hechen Wang , Ruiyang Ma , Ziwei Lin , Xudan Shen , Jiatong Liu , Su Zeng , Sheng Cai
{"title":"开启微型 RNA 和环状 RNA 检测方法之旅","authors":"Zhaleh Asadi Fakhr , Xinlan Zhu , Hechen Wang , Ruiyang Ma , Ziwei Lin , Xudan Shen , Jiatong Liu , Su Zeng , Sheng Cai","doi":"10.1016/j.trac.2024.118035","DOIUrl":null,"url":null,"abstract":"<div><div>Micro-RNAs (miRNAs) and circular-RNAs (circRNAs) are attracting attention as biomarkers for cancer diagnosis due to their distinct biological characteristics. MiRNAs, known for their critical role in gene regulation and tissue specificity, face challenges in detection due to their small size and low abundance in clinical samples. CircRNAs, on the other hand, offer advantages in stability based on their circular structure and are emerging as robust biomarkers. Traditional detection methods have limitations in sensitivity and specificity. Recent developments in isothermal amplification methods promise improved detection capabilities. This review focuses on the innovative primer/probe designs, which enhanced the efficiency of miRNA and circRNA detection in real samples.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"181 ","pages":"Article 118035"},"PeriodicalIF":11.8000,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Embarking on a journey through Micro-RNA and Circular-RNA detection methods\",\"authors\":\"Zhaleh Asadi Fakhr , Xinlan Zhu , Hechen Wang , Ruiyang Ma , Ziwei Lin , Xudan Shen , Jiatong Liu , Su Zeng , Sheng Cai\",\"doi\":\"10.1016/j.trac.2024.118035\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Micro-RNAs (miRNAs) and circular-RNAs (circRNAs) are attracting attention as biomarkers for cancer diagnosis due to their distinct biological characteristics. MiRNAs, known for their critical role in gene regulation and tissue specificity, face challenges in detection due to their small size and low abundance in clinical samples. CircRNAs, on the other hand, offer advantages in stability based on their circular structure and are emerging as robust biomarkers. Traditional detection methods have limitations in sensitivity and specificity. Recent developments in isothermal amplification methods promise improved detection capabilities. This review focuses on the innovative primer/probe designs, which enhanced the efficiency of miRNA and circRNA detection in real samples.</div></div>\",\"PeriodicalId\":439,\"journal\":{\"name\":\"Trends in Analytical Chemistry\",\"volume\":\"181 \",\"pages\":\"Article 118035\"},\"PeriodicalIF\":11.8000,\"publicationDate\":\"2024-11-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Trends in Analytical Chemistry\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0165993624005181\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trends in Analytical Chemistry","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165993624005181","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Embarking on a journey through Micro-RNA and Circular-RNA detection methods
Micro-RNAs (miRNAs) and circular-RNAs (circRNAs) are attracting attention as biomarkers for cancer diagnosis due to their distinct biological characteristics. MiRNAs, known for their critical role in gene regulation and tissue specificity, face challenges in detection due to their small size and low abundance in clinical samples. CircRNAs, on the other hand, offer advantages in stability based on their circular structure and are emerging as robust biomarkers. Traditional detection methods have limitations in sensitivity and specificity. Recent developments in isothermal amplification methods promise improved detection capabilities. This review focuses on the innovative primer/probe designs, which enhanced the efficiency of miRNA and circRNA detection in real samples.
期刊介绍：
TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.
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