开发一种简单经济的分析方法,用于调查虾青素 E/Z- 异构体在食品和化妆品中的分布情况

IF 4 2区 农林科学 Q2 CHEMISTRY, APPLIED Journal of Food Composition and Analysis Pub Date : 2024-11-06 DOI:10.1016/j.jfca.2024.106932
Antara Ghosh , Yasuhiro Nishida , Takashi Maoka , Chiasa Uragami , Hideki Hashimoto , Masaki Honda
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引用次数: 0

摘要

虾青素有许多理论上的E/Z-异构体,特别是由于它们的结构相似和极性,使得分离和准确分析具有挑战性。本研究开发了一种简单、经济高效的反相高效液相色谱(HPLC)方法,可有效分离虾青素的主要异构体(即全E、9Z、13Z和15Z异构体),并评估它们在各种食品、加工产品、食用昆虫和化妆品中的分布情况。我们研究了不同柱温下的各种高效液相色谱柱(C18、C30 和胆固醇键合硅胶)和流动相混合物。我们开发的方法采用 C18 柱(C18-HPLC),在 30 °C、甲醇/H2O(92.5:7.5, v/v)等度模式下,20 分钟内成功分离了虾青素的主要异构体。这种方法还能最大限度地减少来自其他膳食类胡萝卜素的色谱干扰。此外,虽然 C30 色谱柱可以高效分离虾青素异构体,但需要使用毒性大、易挥发的流动相溶剂,如甲基叔丁基醚和二氯甲烷,而且运行时间较长。优化后的 C18-HPLC 方法可准确评估市售食品中虾青素异构体的含量,熟制样品中的异构体比率高于生制样品。总之,所开发的 C18-HPLC 方法是一种简单、高效、经济、快速的虾青素异构体分析工具。
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Development of a simple and economical analytical method for investigating the distribution of astaxanthin E/Z-isomers in foods and cosmetics
Astaxanthin has many theoretical E/Z-isomers, making separation and accurate analysis challenging, especially because of their structural similarities and polarities. The present study developed a simple and cost-effective reversed-phase high-performance liquid chromatography (HPLC) method to separate major astaxanthin isomers efficiently (i.e., the all-E-, 9Z-, 13Z-, and 15Z-isomers) and assess their distribution in various foods, processed products, edible insects, and cosmetics. Various HPLC columns (C18, C30, and cholesteryl-bonded silica) and mobile phase mixtures at different column oven temperatures were examined. Our developed method, using a C18 column (C18-HPLC), successfully separated the major isomers of astaxanthin at 30 °C in isocratic mode with methanol/H2O (92.5:7.5, v/v) within 20 min. This method also minimizes the chromatographic interference from other dietary carotenoids. Additionally, although the C30 column allowed the efficient separation of astaxanthin isomers, it required the use of highly toxic and volatile mobile phase solvents, such as methyl tert-butyl ether and dichloromethane, as well as longer run times. The optimized C18-HPLC method provided an accurate assessment of astaxanthin isomers in commercially available foods, revealing higher isomer ratios in cooked samples than in raw samples. In conclusion, the developed C18-HPLC method is a simple, efficient, cost-effective, and rapid tool for astaxanthin isomer analysis.
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来源期刊
Journal of Food Composition and Analysis
Journal of Food Composition and Analysis 工程技术-食品科技
CiteScore
6.20
自引率
11.60%
发文量
601
审稿时长
53 days
期刊介绍: The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects. The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.
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