{"title":"研究 MpAL1 在 Malus prunifolia 干旱胁迫响应中的作用:洞察 AL 基因家族的功能","authors":"Peien Feng, Jiale Li, Qiuying Ai, Xinchu Li, Jiawei Luo, Yunxiao Liu, Jiakai Liang, Chongrong Chen, Yangjun Zou, Fengwang Ma, Tao Zhao, Yaqiang Sun","doi":"10.1016/j.hpj.2024.07.001","DOIUrl":null,"url":null,"abstract":"Drought stress significantly impedes apple growth, development, and yield, leading to substantial economic losses within the global apple industry. <ce:italic>Malus prunifolia</ce:italic> (<ce:italic>Mp</ce:italic>), a commonly utilized apple rootstock, has shown promise in augmenting cultivated apple resistance to abiotic stress. Although Alfin-like (ALs) proteins have demonstrated pivotal roles in dicotyledonous plants' response to abiotic stresses, knowledge about AL genes in apple rootstocks is limited, and their functions remain largely elusive. In this study, we identified and characterized 10 <ce:italic>MpAL</ce:italic> gene members in the apple rootstock genome, confirming their localization within the nucleus. Our investigation revealed the significant regulation of <ce:italic>MpALs</ce:italic>' expression under drought and abscisic acid (ABA) stresses in <ce:italic>M. prunifolia</ce:italic>. In this study, one of the members, <ce:italic>MpAL1</ce:italic>, was selected for further exploration in <ce:italic>Arabidopsis</ce:italic> and apple to explore its potential function in response to drought and ABA stresses. The results showed that overexpression-<ce:italic>MpAL1</ce:italic> transgenic apple calli grew significantly better than WT and <ce:italic>MpAL1</ce:italic>-RNAi lines, which regulates the accumulation of H<ce:inf loc=\"post\">2</ce:inf>O<ce:inf loc=\"post\">2</ce:inf> and O<ce:inf loc=\"post\">2</ce:inf><ce:sup loc=\"post\">-</ce:sup> levels. Additionally, transgenic <ce:italic>Arabidopsis</ce:italic> plants overexpressing <ce:italic>MpAL1</ce:italic> exhibited positively regulating anti-oxidant enzymes activities under stress treatments. Further study showed that silencing <ce:italic>MpAL1</ce:italic> in apple plants showed obvious chlorosis in leaves, and accumulation of reactive oxygen species under drought stress. Moreover, our detailed analysis established that <ce:italic>MpAL1</ce:italic> regulates several drought and ABA-responsive genes, exerting an influence on their expression in transgenic apple. Collectively, our findings identify <ce:italic>MpAL1</ce:italic> as a positive regulator that increases drought stress in apple, shedding light on its potential significance in bolstering drought resistance in this fruit crop.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"7 1","pages":""},"PeriodicalIF":5.7000,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigating the role of MpAL1 in drought stress responses in Malus prunifolia: Insights into AL gene family functionality\",\"authors\":\"Peien Feng, Jiale Li, Qiuying Ai, Xinchu Li, Jiawei Luo, Yunxiao Liu, Jiakai Liang, Chongrong Chen, Yangjun Zou, Fengwang Ma, Tao Zhao, Yaqiang Sun\",\"doi\":\"10.1016/j.hpj.2024.07.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Drought stress significantly impedes apple growth, development, and yield, leading to substantial economic losses within the global apple industry. <ce:italic>Malus prunifolia</ce:italic> (<ce:italic>Mp</ce:italic>), a commonly utilized apple rootstock, has shown promise in augmenting cultivated apple resistance to abiotic stress. Although Alfin-like (ALs) proteins have demonstrated pivotal roles in dicotyledonous plants' response to abiotic stresses, knowledge about AL genes in apple rootstocks is limited, and their functions remain largely elusive. In this study, we identified and characterized 10 <ce:italic>MpAL</ce:italic> gene members in the apple rootstock genome, confirming their localization within the nucleus. Our investigation revealed the significant regulation of <ce:italic>MpALs</ce:italic>' expression under drought and abscisic acid (ABA) stresses in <ce:italic>M. prunifolia</ce:italic>. In this study, one of the members, <ce:italic>MpAL1</ce:italic>, was selected for further exploration in <ce:italic>Arabidopsis</ce:italic> and apple to explore its potential function in response to drought and ABA stresses. The results showed that overexpression-<ce:italic>MpAL1</ce:italic> transgenic apple calli grew significantly better than WT and <ce:italic>MpAL1</ce:italic>-RNAi lines, which regulates the accumulation of H<ce:inf loc=\\\"post\\\">2</ce:inf>O<ce:inf loc=\\\"post\\\">2</ce:inf> and O<ce:inf loc=\\\"post\\\">2</ce:inf><ce:sup loc=\\\"post\\\">-</ce:sup> levels. Additionally, transgenic <ce:italic>Arabidopsis</ce:italic> plants overexpressing <ce:italic>MpAL1</ce:italic> exhibited positively regulating anti-oxidant enzymes activities under stress treatments. Further study showed that silencing <ce:italic>MpAL1</ce:italic> in apple plants showed obvious chlorosis in leaves, and accumulation of reactive oxygen species under drought stress. Moreover, our detailed analysis established that <ce:italic>MpAL1</ce:italic> regulates several drought and ABA-responsive genes, exerting an influence on their expression in transgenic apple. 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引用次数: 0
摘要
干旱胁迫严重阻碍了苹果的生长、发育和产量,给全球苹果产业造成了巨大的经济损失。苹果砧木 Malus prunifolia(Mp)是一种常用的苹果砧木,在增强栽培苹果对非生物胁迫的抗性方面表现出良好的前景。虽然类阿尔菲蛋白(ALs)在双子叶植物对非生物胁迫的反应中发挥了关键作用,但人们对苹果砧木中的类阿尔菲蛋白基因了解有限,其功能在很大程度上仍然难以捉摸。在这项研究中,我们鉴定并描述了苹果砧木基因组中的 10 个 MpAL 基因成员,确认了它们在细胞核中的定位。我们的研究发现,在干旱和脱落酸(ABA)胁迫下,MpALs的表达对M.本研究选择了其中一个成员 MpAL1 在拟南芥和苹果中进行进一步研究,以探索其在干旱和 ABA 胁迫下的潜在功能。结果表明,过表达-MpAL1转基因苹果胼胝体的生长明显优于WT和MpAL1-RNAi株系,它能调节H2O2和O2-水平的积累。此外,过表达 MpAL1 的转基因拟南芥植株在胁迫处理下表现出正向调节抗氧化酶活性。进一步的研究表明,沉默 MpAL1 的苹果植株在干旱胁迫下会出现明显的叶片萎黄和活性氧积累现象。此外,我们的详细分析还证实,MpAL1 可调控多个干旱和 ABA 响应基因,并对其在转基因苹果中的表达产生影响。总之,我们的研究结果表明,MpAL1 是一种增加苹果干旱胁迫的正调控因子,揭示了它在增强这种水果作物抗旱性方面的潜在意义。
Investigating the role of MpAL1 in drought stress responses in Malus prunifolia: Insights into AL gene family functionality
Drought stress significantly impedes apple growth, development, and yield, leading to substantial economic losses within the global apple industry. Malus prunifolia (Mp), a commonly utilized apple rootstock, has shown promise in augmenting cultivated apple resistance to abiotic stress. Although Alfin-like (ALs) proteins have demonstrated pivotal roles in dicotyledonous plants' response to abiotic stresses, knowledge about AL genes in apple rootstocks is limited, and their functions remain largely elusive. In this study, we identified and characterized 10 MpAL gene members in the apple rootstock genome, confirming their localization within the nucleus. Our investigation revealed the significant regulation of MpALs' expression under drought and abscisic acid (ABA) stresses in M. prunifolia. In this study, one of the members, MpAL1, was selected for further exploration in Arabidopsis and apple to explore its potential function in response to drought and ABA stresses. The results showed that overexpression-MpAL1 transgenic apple calli grew significantly better than WT and MpAL1-RNAi lines, which regulates the accumulation of H2O2 and O2- levels. Additionally, transgenic Arabidopsis plants overexpressing MpAL1 exhibited positively regulating anti-oxidant enzymes activities under stress treatments. Further study showed that silencing MpAL1 in apple plants showed obvious chlorosis in leaves, and accumulation of reactive oxygen species under drought stress. Moreover, our detailed analysis established that MpAL1 regulates several drought and ABA-responsive genes, exerting an influence on their expression in transgenic apple. Collectively, our findings identify MpAL1 as a positive regulator that increases drought stress in apple, shedding light on its potential significance in bolstering drought resistance in this fruit crop.
期刊介绍:
Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.