Zhen Guo, Shenghang Jin, Meiying Yang, Luxuan Fu, Yan Ran, Yan Yu, Weizhong Wang
{"title":"基于 Luminol/PtCo@rGO 和 Au@CNTs 的电化学发光细胞传感器用于超灵敏乳腺癌 CTCs 检测","authors":"Zhen Guo, Shenghang Jin, Meiying Yang, Luxuan Fu, Yan Ran, Yan Yu, Weizhong Wang","doi":"10.1016/j.aca.2024.343452","DOIUrl":null,"url":null,"abstract":"<h3>Background</h3>Breast cancer CTCs have recently been recognized as an emerging biomarker for liquid biopsy of breast cancer. In this work, based on two-dimensional (2D) noble metal PtCo@rGO nanozymes and Au@CNTs bioconjugates, a novel electrochemiluminescence (ECL) cytosensor was was developed in order to detect breast cancer CTCs (MCF-7) ultrasensitively.<h3>Results</h3>The PtCo@rGO nanozymes possessed large specific surface area and high efficiency peroxidase-like activity, which can be used as nanocarriers to anchor and catalyze luminol ECL emission efficiently. Moreover, the PtCo@rGO nanozymes have fractal nanostructures similar to that of CTCs and can capable of enhancing the adhesion of MCF-7 when assembled together with aptamers containing HS-modified epithelial specific cell adhesion molecules (EpCAM, S1). Importantly, the S1/Au@CNTs bioconjugates loaded on the glassy carbon electrode (GCE) can effectively capture MCF-7 cells. Benefiting from the above-mentioned advantages, the ECL cytosensor constructed for MCF-7 cells detection performed well with a wide linear range (2 to 1×10<sup>4</sup> cells mL<sup>-1</sup>) and a low limit of detection (1 cells mL<sup>-1</sup>).<h3>Significance</h3>The designed ECL cytosensor could provide a promising platform for CTC-based liquid biopsy and have broad application prospects in breast cancer early diagnosis and prognostic monitoring.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"16 1","pages":""},"PeriodicalIF":5.7000,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Luminol/PtCo@rGO and Au@CNTs-based electrochemiluminescence cytosensor for ultrasensitive detection of breast cancer CTCs\",\"authors\":\"Zhen Guo, Shenghang Jin, Meiying Yang, Luxuan Fu, Yan Ran, Yan Yu, Weizhong Wang\",\"doi\":\"10.1016/j.aca.2024.343452\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3>Background</h3>Breast cancer CTCs have recently been recognized as an emerging biomarker for liquid biopsy of breast cancer. In this work, based on two-dimensional (2D) noble metal PtCo@rGO nanozymes and Au@CNTs bioconjugates, a novel electrochemiluminescence (ECL) cytosensor was was developed in order to detect breast cancer CTCs (MCF-7) ultrasensitively.<h3>Results</h3>The PtCo@rGO nanozymes possessed large specific surface area and high efficiency peroxidase-like activity, which can be used as nanocarriers to anchor and catalyze luminol ECL emission efficiently. Moreover, the PtCo@rGO nanozymes have fractal nanostructures similar to that of CTCs and can capable of enhancing the adhesion of MCF-7 when assembled together with aptamers containing HS-modified epithelial specific cell adhesion molecules (EpCAM, S1). Importantly, the S1/Au@CNTs bioconjugates loaded on the glassy carbon electrode (GCE) can effectively capture MCF-7 cells. Benefiting from the above-mentioned advantages, the ECL cytosensor constructed for MCF-7 cells detection performed well with a wide linear range (2 to 1×10<sup>4</sup> cells mL<sup>-1</sup>) and a low limit of detection (1 cells mL<sup>-1</sup>).<h3>Significance</h3>The designed ECL cytosensor could provide a promising platform for CTC-based liquid biopsy and have broad application prospects in breast cancer early diagnosis and prognostic monitoring.\",\"PeriodicalId\":240,\"journal\":{\"name\":\"Analytica Chimica Acta\",\"volume\":\"16 1\",\"pages\":\"\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2024-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytica Chimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.aca.2024.343452\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytica Chimica Acta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.aca.2024.343452","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Luminol/PtCo@rGO and Au@CNTs-based electrochemiluminescence cytosensor for ultrasensitive detection of breast cancer CTCs
Background
Breast cancer CTCs have recently been recognized as an emerging biomarker for liquid biopsy of breast cancer. In this work, based on two-dimensional (2D) noble metal PtCo@rGO nanozymes and Au@CNTs bioconjugates, a novel electrochemiluminescence (ECL) cytosensor was was developed in order to detect breast cancer CTCs (MCF-7) ultrasensitively.
Results
The PtCo@rGO nanozymes possessed large specific surface area and high efficiency peroxidase-like activity, which can be used as nanocarriers to anchor and catalyze luminol ECL emission efficiently. Moreover, the PtCo@rGO nanozymes have fractal nanostructures similar to that of CTCs and can capable of enhancing the adhesion of MCF-7 when assembled together with aptamers containing HS-modified epithelial specific cell adhesion molecules (EpCAM, S1). Importantly, the S1/Au@CNTs bioconjugates loaded on the glassy carbon electrode (GCE) can effectively capture MCF-7 cells. Benefiting from the above-mentioned advantages, the ECL cytosensor constructed for MCF-7 cells detection performed well with a wide linear range (2 to 1×104 cells mL-1) and a low limit of detection (1 cells mL-1).
Significance
The designed ECL cytosensor could provide a promising platform for CTC-based liquid biopsy and have broad application prospects in breast cancer early diagnosis and prognostic monitoring.
期刊介绍:
Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review.
Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.