便捷地制备针对牙周炎生物标志物 S100A8 的新型重组抗体。

IF 2 4区生物学 Q3 BIOCHEMICAL RESEARCH METHODS Preparative Biochemistry & Biotechnology Pub Date : 2024-11-20 DOI:10.1080/10826068.2024.2430615

Hui-Seon Yun, Eun-Jung Kim, Byung-Gee Kim, Hee-Jin Jeong

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引用次数: 0

摘要

S100A8 是牙周炎的生物标志物，参与炎症过程，因此对它的检测非常重要。在这项研究中，我们通过哺乳动物细胞培养制备了重组 5A11（r5A11）。通过转染、悬浮细胞培养和纯化三个步骤，我们方便地制备出了高产率和高纯度的 r5A11。基于 r5A11 的免疫测定的检测限为 1.7 ± 0.2 × 10-1 ng/mL，高于市售的抗 S100A8 抗体。这些研究结果表明，这种新型抗体可用于各种研究应用和简单灵敏的 S100A8 检测。

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Convenient production of a novel recombinant antibody against periodontitis biomarker S100A8.

S100A8 serves as a biomarker for periodontitis and is involved in inflammatory processes, making its detection highly important. In this study, we produced recombinant 5A11 (r5A11) through mammalian cell culture. By employing a three-step process of transfection, suspension cell culture, and purification, we conveniently produced r5A11 with high yield and purity. The limit of detection for the r5A11-based immunoassay was 1.7 ± 0.2 × 10^-1ng/mL, which was higher than that of the commercially available anti-S100A8 antibody. These findings suggest the potential use of this novel antibody in various research applications and practical approaches for simple and sensitive S100A8 detection.

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来源期刊

Preparative Biochemistry & Biotechnology 工程技术-生化研究方法

CiteScore

4.90

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： Preparative Biochemistry & Biotechnology is an international forum for rapid dissemination of high quality research results dealing with all aspects of preparative techniques in biochemistry, biotechnology and other life science disciplines.