通过优化冷却条件延长种马精液的保存时间。

IF 1.3 3区 农林科学 Q2 VETERINARY SCIENCES Journal of Equine Veterinary Science Pub Date : 2024-11-18 DOI:10.1016/j.jevs.2024.105243
Jillian E Guertin, Joao de Agostini Losano, Sophia Salazar, Justin Callaham, Bradford W Daigneault
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引用次数: 0

摘要

马匹辅助生殖技术(ART)成功的瓶颈包括公马精子长时间储存的条件不理想。公马精子在运输过程中会被放置在冷却装置中,以保持温度长达 48 小时。延长冷却精液的储存时间,同时保持可接受的活力,将缓解辅助生殖技术的物流难题。为了验证外部调节装运容器温度可延长冷却种公马精液储存时间的假设,我们进行了实验。最初的实验确定了在-20°C或-80°C下预先冷冻冷却罐对精子活力的影响。在 INRA96 中延长新鲜精子的储存时间,并将其置于商用 Equitainers 中 3.5 天(84 小时)。精子在-20°C等温容器中保存 60 小时后,其总活力和渐进活力均高于-80°C 条件下的精子(分别为 63%, 29% vs. 32%, 17%)。对装有-20°C冷冻罐的等温器进行的内部监测发现,20°C是精子活力迅速下降的阈值。在第二个实验中,精子被保存在装有 -20°C 冷冻罐的 Equitainer 中,并置于两种不同的条件下:1)环境温度 48 小时(23°C);或 2)5°C 外部温度调节,最长 8 天,或当总活力降至 50%以下时。每隔 24 小时测定一次精子运动学。在外部温度调节(5°C)下保存 7 天以上的样本中,总活力仍保持在 50%以上。这些研究结果表明,通过改善贮存条件的温度维持,冷却后的种公马精液可以超越传统的 48 小时限制。
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Prolonged maintenance of stallion semen by optimization of cooling conditions.

Bottlenecks to the success of equine assisted reproductive technologies (ART) include suboptimal conditions for prolonged storage of stallion sperm. Shipped stallion sperm are transported in cooling devices designed to maintain temperature for up to 48 h. Increasing the storage time of cooled semen while maintaining acceptable motility would relieve logistical ART challenges. Experiments were conducted to test the hypothesis that external regulation of shipment container temperature would prolong storage time of cooled stallion semen. Initial experiments determined the effect of pre-freezing cooling cans at -20°C or -80°C on sperm motility. Fresh sperm was extended in INRA96 and placed in commercial Equitainers for 3.5 days (84 h). Quantification of sperm kinematics was determined every 12 h. Sperm held in Equitainers with -20°C cans maintained higher total and progressive motility than -80°C conditions at 60 h (63%, 29% vs. 32%, 17%, respectively). Internal monitoring of Equitainers containing -20°C freezer can temperature identified 20°C as the threshold for rapidly decreased motility. In the second experiment, sperm were maintained in Equitainers containing -20°C freezer cans and placed in two different conditions: 1) ambient temperature for 48 h (23°C) or 2) 5°C for external temperature regulation up to 8 days, or when total motility dropped below 50%. Sperm kinematics was determined every 24 h. Total motility from samples held with external temperature regulation (5°C) remained above 50% for more than 7 days. These findings suggest that cooled stallion semen can be advanced beyond traditional 48 h constraints by improving temperature maintenance of storage conditions.

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来源期刊
Journal of Equine Veterinary Science
Journal of Equine Veterinary Science 农林科学-兽医学
CiteScore
2.70
自引率
7.70%
发文量
249
审稿时长
77 days
期刊介绍: Journal of Equine Veterinary Science (JEVS) is an international publication designed for the practicing equine veterinarian, equine researcher, and other equine health care specialist. Published monthly, each issue of JEVS includes original research, reviews, case reports, short communications, and clinical techniques from leaders in the equine veterinary field, covering such topics as laminitis, reproduction, infectious disease, parasitology, behavior, podology, internal medicine, surgery and nutrition.
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