{"title":"超声辅助三聚磷酸钠改善了低盐条件下肌原纤维蛋白的凝胶特性。","authors":"Rui Li, Xiaoqi Li, Chen Wang, Guiming Zhang, Yabin Niu, Fashan Wei, Lin Chen, Xianchao Feng","doi":"10.1016/j.meatsci.2024.109712","DOIUrl":null,"url":null,"abstract":"<p><p>Excessive salt intake is associated with increased risks of cardiovascular disease, while directly reducing salt content significantly decreased the quality of meat. The objective of this study was to investigate the synergistic effects of sodium tripolyphosphate (STP) and ultrasound treatment on the gel properties of MP under low-salt conditions (0.3 M NaCl). The results of FTIR spectra showed that P<sub>3</sub>O<sub>10</sub><sup>5-</sup> group of STP bound to -NH<sub>2</sub> or -OH group of MP form C-N-P or C-O-P bond, indicating the STP was successfully introduced to MP. The addition of STP significantly increased the absolute value of Zeta-potential, suggesting that the presence of STP increased the electrostatic interaction of MP-MP. Importantly, STP combined with ultrasound treatment under low salt condition (STP-U<sub>0.3</sub>) significantly increased solubility and decreased particle size of MP. Besides, STP-U<sub>0.3</sub> treatment also promoted the exposure of hydrophobic groups and improved the rheological behavior of MP, resulting in the highest gel strength (37.78 ± 0.71 g) and the lowest cooking loss (26.73 ± 0.90 %) especially in 10 mM STP combined 100 W ultrasound treatment. These results corresponded by the gradually increases of α-helix content and the decrease of tryptophan fluorescence intensity. Furthermore, results of SEM illustrated that STP-U<sub>0.3</sub> treatment contributed to formation of more homogeneous and dense gel network of MP gel. The above results displayed that the STP-U treatment under 0.3 M NaCl resulted in an equivalent effect to control group of 0.6 M NaCl, indicating that the combined application of STP and ultrasound has a promising potential in the low-salt meat processing industry.</p>","PeriodicalId":389,"journal":{"name":"Meat Science","volume":"220 ","pages":"109712"},"PeriodicalIF":7.1000,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The improved gel properties of myofibrillar protein under low salt condition by ultrasound-assisted sodium tripolyphosphate.\",\"authors\":\"Rui Li, Xiaoqi Li, Chen Wang, Guiming Zhang, Yabin Niu, Fashan Wei, Lin Chen, Xianchao Feng\",\"doi\":\"10.1016/j.meatsci.2024.109712\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Excessive salt intake is associated with increased risks of cardiovascular disease, while directly reducing salt content significantly decreased the quality of meat. The objective of this study was to investigate the synergistic effects of sodium tripolyphosphate (STP) and ultrasound treatment on the gel properties of MP under low-salt conditions (0.3 M NaCl). The results of FTIR spectra showed that P<sub>3</sub>O<sub>10</sub><sup>5-</sup> group of STP bound to -NH<sub>2</sub> or -OH group of MP form C-N-P or C-O-P bond, indicating the STP was successfully introduced to MP. The addition of STP significantly increased the absolute value of Zeta-potential, suggesting that the presence of STP increased the electrostatic interaction of MP-MP. Importantly, STP combined with ultrasound treatment under low salt condition (STP-U<sub>0.3</sub>) significantly increased solubility and decreased particle size of MP. Besides, STP-U<sub>0.3</sub> treatment also promoted the exposure of hydrophobic groups and improved the rheological behavior of MP, resulting in the highest gel strength (37.78 ± 0.71 g) and the lowest cooking loss (26.73 ± 0.90 %) especially in 10 mM STP combined 100 W ultrasound treatment. These results corresponded by the gradually increases of α-helix content and the decrease of tryptophan fluorescence intensity. Furthermore, results of SEM illustrated that STP-U<sub>0.3</sub> treatment contributed to formation of more homogeneous and dense gel network of MP gel. The above results displayed that the STP-U treatment under 0.3 M NaCl resulted in an equivalent effect to control group of 0.6 M NaCl, indicating that the combined application of STP and ultrasound has a promising potential in the low-salt meat processing industry.</p>\",\"PeriodicalId\":389,\"journal\":{\"name\":\"Meat Science\",\"volume\":\"220 \",\"pages\":\"109712\"},\"PeriodicalIF\":7.1000,\"publicationDate\":\"2024-11-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Meat Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.meatsci.2024.109712\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Meat Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.meatsci.2024.109712","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
摘要
盐摄入过多会增加心血管疾病的风险,而直接降低盐含量则会显著降低肉的品质。本研究旨在探讨在低盐条件(0.3 M NaCl)下,三聚磷酸钠(STP)和超声处理对 MP 凝胶特性的协同效应。傅立叶变换红外光谱结果表明,STP的P3O105-基团与MP的-NH2或-OH基团结合形成C-N-P或C-O-P键,表明STP成功引入到MP中。STP 的加入明显增加了 Zeta 电位的绝对值,表明 STP 的存在增加了 MP-MP 的静电作用。重要的是,STP 与低盐条件下的超声处理(STP-U0.3)相结合,可明显提高 MP 的溶解度并减小其粒径。此外,STP-U0.3 处理还促进了疏水基团的暴露,改善了 MP 的流变行为,从而使其凝胶强度最高(37.78 ± 0.71 g),蒸煮损失最低(26.73 ± 0.90 %),尤其是在 10 mM STP 结合 100 W 超声波处理的情况下。这些结果与α-螺旋含量的逐渐增加和色氨酸荧光强度的降低相对应。此外,扫描电镜的结果表明,STP-U0.3 处理有助于 MP 凝胶形成更均匀致密的凝胶网络。上述结果表明,STP-U 在 0.3 M NaCl 条件下的处理效果与 0.6 M NaCl 条件下的对照组效果相当,这表明 STP 和超声波的联合应用在低盐肉类加工业中具有广阔的前景。
The improved gel properties of myofibrillar protein under low salt condition by ultrasound-assisted sodium tripolyphosphate.
Excessive salt intake is associated with increased risks of cardiovascular disease, while directly reducing salt content significantly decreased the quality of meat. The objective of this study was to investigate the synergistic effects of sodium tripolyphosphate (STP) and ultrasound treatment on the gel properties of MP under low-salt conditions (0.3 M NaCl). The results of FTIR spectra showed that P3O105- group of STP bound to -NH2 or -OH group of MP form C-N-P or C-O-P bond, indicating the STP was successfully introduced to MP. The addition of STP significantly increased the absolute value of Zeta-potential, suggesting that the presence of STP increased the electrostatic interaction of MP-MP. Importantly, STP combined with ultrasound treatment under low salt condition (STP-U0.3) significantly increased solubility and decreased particle size of MP. Besides, STP-U0.3 treatment also promoted the exposure of hydrophobic groups and improved the rheological behavior of MP, resulting in the highest gel strength (37.78 ± 0.71 g) and the lowest cooking loss (26.73 ± 0.90 %) especially in 10 mM STP combined 100 W ultrasound treatment. These results corresponded by the gradually increases of α-helix content and the decrease of tryptophan fluorescence intensity. Furthermore, results of SEM illustrated that STP-U0.3 treatment contributed to formation of more homogeneous and dense gel network of MP gel. The above results displayed that the STP-U treatment under 0.3 M NaCl resulted in an equivalent effect to control group of 0.6 M NaCl, indicating that the combined application of STP and ultrasound has a promising potential in the low-salt meat processing industry.
期刊介绍:
The aim of Meat Science is to serve as a suitable platform for the dissemination of interdisciplinary and international knowledge on all factors influencing the properties of meat. While the journal primarily focuses on the flesh of mammals, contributions related to poultry will be considered if they enhance the overall understanding of the relationship between muscle nature and meat quality post mortem. Additionally, papers on large birds (e.g., emus, ostriches) as well as wild-captured mammals and crocodiles will be welcomed.