Ionica Iancu, Violeta Igna, Sebastian Alexandru Popa, Kálmán Imre, Corina Pascu, Luminița Costinar, Janos Degi, Alexandru Gligor, Vlad Iorgoni, Corina Badea, Ileana Nichita, Viorel Herman
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Thus, Staphylococcus aureus (129/146; 88.4%) was identified as the predominant pathogen followed by Streptococcus spp. (14/146; 9.6%) Enterococcus spp. (7/146; 4.8%), Pseudomonas aeruginosa (6/146; 4.1%) and Klebsiella pneumoniae (5/146; 3.4%). Antimicrobial susceptibility of isolated strains of Staphylococcus aureus, Streptococcus spp. and Enterococcus spp. was also tested. The results showed varying resistance patterns depending on the farm and microorganism. In heard A the highest resistance of Staphylococcus aureus was identified to polymyxin B (79.7%) followed by novobiocin (73.4%) and tetracycline (45.3%); Streptococcus spp. to streptomycin (100%), novobiocin (100%), tetracycline (100%) and Enterococcus spp. to tylosin (100%), streptomycin (75.0%) and tetracycline (75.0%). 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引用次数: 0
摘要
本研究调查了罗马尼亚西南部Țurcana 羊群亚临床乳腺炎的发病率和病因。亚临床乳腺炎的检测采用了 Milchtest 和加利福尼亚乳腺炎测试（CMT）。对三个哺乳期的 360 份牛奶样本进行了分析。在 146 份（40.6%）羊奶样本中发现了亚临床乳腺炎。只有从确诊患有亚临床乳腺炎的绵羊身上采集的牛奶样本（n = 146）才被考虑用于微生物分析。因此，金黄色葡萄球菌（129/146；88.4%）被确定为主要病原体，其次是链球菌属（14/146；9.6%）、肠球菌属（7/146；4.8%）、铜绿假单胞菌（6/146；4.1%）和肺炎克雷伯菌（5/146；3.4%）。此外，还检测了金黄色葡萄球菌、链球菌属和肠球菌属分离菌株的抗菌药敏感性。结果显示，不同农场和微生物的耐药性模式各不相同。在 A 群中，金黄色葡萄球菌对多粘菌素 B 的耐药性最高（79.7%），其次是新生物素（73.4%）和四环素（45.3%）；链球菌对链霉素（100%）、新生物素（100%）和四环素（100%）产生耐药性；肠球菌对泰乐菌素（100%）、链霉素（75.0%）和四环素（75.0%）产生耐药性。在 B 组中，金黄色葡萄球菌对多粘菌素 B（86.3%）、新生物素（74.5%）和氯霉素（39.2%）的耐药率较高；链球菌对链霉素（100%）、新生物素（100%）和四环素（100%）的耐药率较高；肠球菌对庆大霉素（100%）、链霉素（66.7%）和红霉素（66.7%）的耐药率较高。获得的初步结果表明，未来治疗羊乳腺炎的方法可能存在无效的潜在风险，并表明未经巴氏杀菌的生牛奶中可能含有抗菌素耐药性病原体，对公共卫生构成威胁。不过，要得出全面的结论，还需要进一步开展抗药性基因的分子研究。
Etiology and antimicrobial resistance of subclinical mastitis pathogens Staphylococcus aureus, Streptococcus spp. and Enterococcus spp. in sheep milk.
The present study investigates the prevalence and etiology of subclinical mastitis in Țurcana sheep flocks located in south-western Romania. Milchtest and California Mastitis Test (CMT), were used for the detection of subclinical mastitis. A number of 360 milk samples across three lactation phases were analyzed. Subclinical mastitis was identified in 146 (40.6%) of sheep milk samples. Only milk samples (n = 146) collected from sheep diagnosed with subclinical mastitis were considered for microbiological analyses. Thus, Staphylococcus aureus (129/146; 88.4%) was identified as the predominant pathogen followed by Streptococcus spp. (14/146; 9.6%) Enterococcus spp. (7/146; 4.8%), Pseudomonas aeruginosa (6/146; 4.1%) and Klebsiella pneumoniae (5/146; 3.4%). Antimicrobial susceptibility of isolated strains of Staphylococcus aureus, Streptococcus spp. and Enterococcus spp. was also tested. The results showed varying resistance patterns depending on the farm and microorganism. In heard A the highest resistance of Staphylococcus aureus was identified to polymyxin B (79.7%) followed by novobiocin (73.4%) and tetracycline (45.3%); Streptococcus spp. to streptomycin (100%), novobiocin (100%), tetracycline (100%) and Enterococcus spp. to tylosin (100%), streptomycin (75.0%) and tetracycline (75.0%). In herd B, Staphylococcus aureus showed high resistance rates to polymyxin B (86.3%), novobiocin (74.5%) and cloxacillin (39.2%); Streptococcus spp. strains to streptomycin (100%), novobiocin (100%), tetracycline (100%) and Enterococcus spp. to gentamicin (100%), streptomycin (66.7%) and erythromycin (66.7%). The obtained preliminary results indicate a potential risk that future treatments of sheep mastitis may not be effective, and demonstrated that raw unpasteurized milk can harbor antimicrobial resistance pathogens posing a threat to public health. However, further investigations involving molecular research on resistance genes are required to draw comprehensive conclusions.
期刊介绍：
Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial.
The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.
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