Wenwen Wang, Dexin Liu, Tingfu Zhang, Kai Guo, Xueying Liu, Dajun Liu, Lei Chen, Jinming Yang, Zhonghua Teng, Ying Zou, Junrui Ma, Yi Wang, Xinrui Yang, Xin Guo, Xiaoting Sun, Jian Zhang, Yuehua Xiao, Andrew H. Paterson, Zhengsheng Zhang
{"title":"GhROPGEF5 的自然变异可使棉纤维更长、更结实。","authors":"Wenwen Wang, Dexin Liu, Tingfu Zhang, Kai Guo, Xueying Liu, Dajun Liu, Lei Chen, Jinming Yang, Zhonghua Teng, Ying Zou, Junrui Ma, Yi Wang, Xinrui Yang, Xin Guo, Xiaoting Sun, Jian Zhang, Yuehua Xiao, Andrew H. Paterson, Zhengsheng Zhang","doi":"10.1111/nph.20286","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>\n </p><ul>\n \n <li>Length and strength are key parameters impacting the quality of textiles that can be produced from cotton fibers, and therefore are important considerations in cotton breeding.</li>\n \n <li>Through map-based cloning and function analysis, we demonstrated that <i>GhROPGEF5</i>, encoding a ROP guanine nucleotide exchange factor, was the gene controlling fiber length and strength at <i>qFS</i><sub><i>A10.1</i></sub>. Evolutionary analysis revealed that a base deletion in the third exon of <i>GhROPGEF5</i> resulting in superior fiber length and strength was a rare mutation occurring in a tiny percentage of Upland cottons, with reduced fiber yield hindering its spread.</li>\n \n <li>GhROPGEF5 interacted with and activated GhROP10. Knockout or mutation of <i>GhROPGEF5</i> resulted a loss of the ability to activate GhROP10. Knockout of <i>GhROPGEF5</i> or <i>GhROP10</i> affected the expression of many downstream genes associated with fiber elongation and secondary wall deposition, prolonged fiber elongation and delayed secondary wall deposition, producing denser fiber helices and increasing fiber length and strength.</li>\n \n <li>These results revealed new molecular aspects of fiber development and revealed a rare favorable allele for improving fiber quality in cotton breeding.</li>\n </ul>\n </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 3","pages":"1090-1105"},"PeriodicalIF":8.1000,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Natural variation in GhROPGEF5 contributes to longer and stronger cotton fibers\",\"authors\":\"Wenwen Wang, Dexin Liu, Tingfu Zhang, Kai Guo, Xueying Liu, Dajun Liu, Lei Chen, Jinming Yang, Zhonghua Teng, Ying Zou, Junrui Ma, Yi Wang, Xinrui Yang, Xin Guo, Xiaoting Sun, Jian Zhang, Yuehua Xiao, Andrew H. Paterson, Zhengsheng Zhang\",\"doi\":\"10.1111/nph.20286\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>\\n </p><ul>\\n \\n <li>Length and strength are key parameters impacting the quality of textiles that can be produced from cotton fibers, and therefore are important considerations in cotton breeding.</li>\\n \\n <li>Through map-based cloning and function analysis, we demonstrated that <i>GhROPGEF5</i>, encoding a ROP guanine nucleotide exchange factor, was the gene controlling fiber length and strength at <i>qFS</i><sub><i>A10.1</i></sub>. Evolutionary analysis revealed that a base deletion in the third exon of <i>GhROPGEF5</i> resulting in superior fiber length and strength was a rare mutation occurring in a tiny percentage of Upland cottons, with reduced fiber yield hindering its spread.</li>\\n \\n <li>GhROPGEF5 interacted with and activated GhROP10. Knockout or mutation of <i>GhROPGEF5</i> resulted a loss of the ability to activate GhROP10. Knockout of <i>GhROPGEF5</i> or <i>GhROP10</i> affected the expression of many downstream genes associated with fiber elongation and secondary wall deposition, prolonged fiber elongation and delayed secondary wall deposition, producing denser fiber helices and increasing fiber length and strength.</li>\\n \\n <li>These results revealed new molecular aspects of fiber development and revealed a rare favorable allele for improving fiber quality in cotton breeding.</li>\\n </ul>\\n </div>\",\"PeriodicalId\":214,\"journal\":{\"name\":\"New Phytologist\",\"volume\":\"245 3\",\"pages\":\"1090-1105\"},\"PeriodicalIF\":8.1000,\"publicationDate\":\"2024-11-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"New Phytologist\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.20286\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Phytologist","FirstCategoryId":"99","ListUrlMain":"https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.20286","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Natural variation in GhROPGEF5 contributes to longer and stronger cotton fibers
Length and strength are key parameters impacting the quality of textiles that can be produced from cotton fibers, and therefore are important considerations in cotton breeding.
Through map-based cloning and function analysis, we demonstrated that GhROPGEF5, encoding a ROP guanine nucleotide exchange factor, was the gene controlling fiber length and strength at qFSA10.1. Evolutionary analysis revealed that a base deletion in the third exon of GhROPGEF5 resulting in superior fiber length and strength was a rare mutation occurring in a tiny percentage of Upland cottons, with reduced fiber yield hindering its spread.
GhROPGEF5 interacted with and activated GhROP10. Knockout or mutation of GhROPGEF5 resulted a loss of the ability to activate GhROP10. Knockout of GhROPGEF5 or GhROP10 affected the expression of many downstream genes associated with fiber elongation and secondary wall deposition, prolonged fiber elongation and delayed secondary wall deposition, producing denser fiber helices and increasing fiber length and strength.
These results revealed new molecular aspects of fiber development and revealed a rare favorable allele for improving fiber quality in cotton breeding.
期刊介绍：
New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.
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