Yuting Wang, Ning Wang, Boyu Liu, Cuiping Han, Tong Wang, Mingzhe Pan, Dianyu Yu
{"title":"欧姆加热辅助糖化反应对大豆分离蛋白壳聚糖复合物性质的影响","authors":"Yuting Wang, Ning Wang, Boyu Liu, Cuiping Han, Tong Wang, Mingzhe Pan, Dianyu Yu","doi":"10.1016/j.ijbiomac.2024.137859","DOIUrl":null,"url":null,"abstract":"<p><p>The purpose of this study was to investigate the progress of glycation reaction reactions by conventional heating and ohmic heating (OH) treatment, and the effect of different electric field intensities on the structure, physical and chemical and functional properties of glycosylated proteins. The findings demonstrated that OH treatment was more efficacious than conventional heating in reducing the free amino group and increasing the absorbance at 420 nm. Concurrently, the α-helix and β-sheet content of soy protein isolate (SPI)-chitosan (CS) complexes exhibited a reduction to 18.01 % and 28.67 %, respectively, while the UV absorption peak demonstrated an increase in conjunction with the escalation of electric field intensity. When the electric field intensity was 6 V/cm, the emulsification activity index and emulsion stability index of SPI-CS complexes were found to be 95.52 m<sup>2</sup>/g and 55.60 min, respectively. The foaming capacity and foaming stability were found to be 148.33 % and 115.59 % respectively, while the solubility was also up to 91.37 %. Additionally, the air/water interface properties demonstrated a notable enhancement. The functional properties of the complexes were demonstrably enhanced following the application of an OH treatment. The aforementioned statement provided a theoretical foundation for the implementation of OH treatment.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137859"},"PeriodicalIF":7.7000,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of ohmic heating-assisted glycation reaction on the properties of soybean protein isolate-chitosan complexes.\",\"authors\":\"Yuting Wang, Ning Wang, Boyu Liu, Cuiping Han, Tong Wang, Mingzhe Pan, Dianyu Yu\",\"doi\":\"10.1016/j.ijbiomac.2024.137859\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The purpose of this study was to investigate the progress of glycation reaction reactions by conventional heating and ohmic heating (OH) treatment, and the effect of different electric field intensities on the structure, physical and chemical and functional properties of glycosylated proteins. The findings demonstrated that OH treatment was more efficacious than conventional heating in reducing the free amino group and increasing the absorbance at 420 nm. Concurrently, the α-helix and β-sheet content of soy protein isolate (SPI)-chitosan (CS) complexes exhibited a reduction to 18.01 % and 28.67 %, respectively, while the UV absorption peak demonstrated an increase in conjunction with the escalation of electric field intensity. When the electric field intensity was 6 V/cm, the emulsification activity index and emulsion stability index of SPI-CS complexes were found to be 95.52 m<sup>2</sup>/g and 55.60 min, respectively. The foaming capacity and foaming stability were found to be 148.33 % and 115.59 % respectively, while the solubility was also up to 91.37 %. Additionally, the air/water interface properties demonstrated a notable enhancement. The functional properties of the complexes were demonstrably enhanced following the application of an OH treatment. The aforementioned statement provided a theoretical foundation for the implementation of OH treatment.</p>\",\"PeriodicalId\":333,\"journal\":{\"name\":\"International Journal of Biological Macromolecules\",\"volume\":\" \",\"pages\":\"137859\"},\"PeriodicalIF\":7.7000,\"publicationDate\":\"2024-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biological Macromolecules\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.ijbiomac.2024.137859\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biological Macromolecules","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.ijbiomac.2024.137859","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Effect of ohmic heating-assisted glycation reaction on the properties of soybean protein isolate-chitosan complexes.
The purpose of this study was to investigate the progress of glycation reaction reactions by conventional heating and ohmic heating (OH) treatment, and the effect of different electric field intensities on the structure, physical and chemical and functional properties of glycosylated proteins. The findings demonstrated that OH treatment was more efficacious than conventional heating in reducing the free amino group and increasing the absorbance at 420 nm. Concurrently, the α-helix and β-sheet content of soy protein isolate (SPI)-chitosan (CS) complexes exhibited a reduction to 18.01 % and 28.67 %, respectively, while the UV absorption peak demonstrated an increase in conjunction with the escalation of electric field intensity. When the electric field intensity was 6 V/cm, the emulsification activity index and emulsion stability index of SPI-CS complexes were found to be 95.52 m2/g and 55.60 min, respectively. The foaming capacity and foaming stability were found to be 148.33 % and 115.59 % respectively, while the solubility was also up to 91.37 %. Additionally, the air/water interface properties demonstrated a notable enhancement. The functional properties of the complexes were demonstrably enhanced following the application of an OH treatment. The aforementioned statement provided a theoretical foundation for the implementation of OH treatment.
期刊介绍:
The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.