{"title":"出现携带 NDM-5、KPC-2 和 OXA-181 三种不同质粒的耐多药大肠杆菌 ST8346 分离物。","authors":"Ping-Yuan Chen, Yu-Chin Chen, Che-Ming Chiang, Chi-Chung Chen, Chih-Cheng Lai, Chia-Hung Tsai, Chung-Han Ho, Po-Yu Chen, Hung-Jen Tang, Julie Chi Chow, Tu-Hsuan Chang","doi":"10.1016/j.jiph.2024.102596","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>E. coli ST8346 is a unique strain associated with the potential carriage of multiple carbapenemases. Three unique E. coli ST8346 isolates were identified, each concurrently harboring multiple carbapenemases, including bla<sub>NDM-5</sub>, bla<sub>KPC-2</sub>, and/or bla<sub>OXA-181</sub>. This study aimed to characterize the genetic and plasmid structures of these isolates to understand their transmission and resistance mechanisms.</p><p><strong>Methods: </strong>Antibiotic resistance profiles, genetic relatedness, and plasmid structures were determined using antibiotic susceptibility testing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), S1 nuclease PFGE, and long-read sequencing.</p><p><strong>Results: </strong>All the strains were carbapenem resistant. PFGE revealed close genetic relationships among the isolates, despite the lack of geographical or epidemiological connections. bla<sub>NDM-5</sub>, bla<sub>KPC-2</sub>, and bla<sub>OXA-181</sub> were located on separate plasmids. Plasmids harboring bla<sub>NDM-5</sub> showed genetic similarities to bla<sub>NDM-5</sub>-bearing IncF plasmids in the United Kingdom. The IncA/C plasmids harboring bla<sub>KPC-2</sub> had identical sequences resembling a plasmid from a K. pneumoniae strain in Taiwan, except for the bla<sub>KPC-2</sub> region, which matched a strain from China, indicating a hybrid plasmid.</p><p><strong>Conclusion: </strong>This study is the first to identify and characterize the coexistence of bla<sub>NDM-5</sub>, bla<sub>KPC-2</sub>, and bla<sub>OXA-181</sub> in E. coli ST8346 isolates. The spread appears to be due to independent acquisition events. We identified the putative origins of these plasmids and detected a possible recombination event in a novel IncA/C plasmid. These findings emphasize the importance of ongoing surveillance and further investigations.</p>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"17 12","pages":"102596"},"PeriodicalIF":4.7000,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Emergence of multidrug-resistant E. coli ST8346 isolates carrying three distinct plasmids with NDM-5, KPC-2, and OXA-181.\",\"authors\":\"Ping-Yuan Chen, Yu-Chin Chen, Che-Ming Chiang, Chi-Chung Chen, Chih-Cheng Lai, Chia-Hung Tsai, Chung-Han Ho, Po-Yu Chen, Hung-Jen Tang, Julie Chi Chow, Tu-Hsuan Chang\",\"doi\":\"10.1016/j.jiph.2024.102596\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>E. coli ST8346 is a unique strain associated with the potential carriage of multiple carbapenemases. Three unique E. coli ST8346 isolates were identified, each concurrently harboring multiple carbapenemases, including bla<sub>NDM-5</sub>, bla<sub>KPC-2</sub>, and/or bla<sub>OXA-181</sub>. This study aimed to characterize the genetic and plasmid structures of these isolates to understand their transmission and resistance mechanisms.</p><p><strong>Methods: </strong>Antibiotic resistance profiles, genetic relatedness, and plasmid structures were determined using antibiotic susceptibility testing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), S1 nuclease PFGE, and long-read sequencing.</p><p><strong>Results: </strong>All the strains were carbapenem resistant. PFGE revealed close genetic relationships among the isolates, despite the lack of geographical or epidemiological connections. bla<sub>NDM-5</sub>, bla<sub>KPC-2</sub>, and bla<sub>OXA-181</sub> were located on separate plasmids. Plasmids harboring bla<sub>NDM-5</sub> showed genetic similarities to bla<sub>NDM-5</sub>-bearing IncF plasmids in the United Kingdom. The IncA/C plasmids harboring bla<sub>KPC-2</sub> had identical sequences resembling a plasmid from a K. pneumoniae strain in Taiwan, except for the bla<sub>KPC-2</sub> region, which matched a strain from China, indicating a hybrid plasmid.</p><p><strong>Conclusion: </strong>This study is the first to identify and characterize the coexistence of bla<sub>NDM-5</sub>, bla<sub>KPC-2</sub>, and bla<sub>OXA-181</sub> in E. coli ST8346 isolates. The spread appears to be due to independent acquisition events. We identified the putative origins of these plasmids and detected a possible recombination event in a novel IncA/C plasmid. These findings emphasize the importance of ongoing surveillance and further investigations.</p>\",\"PeriodicalId\":16087,\"journal\":{\"name\":\"Journal of Infection and Public Health\",\"volume\":\"17 12\",\"pages\":\"102596\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2024-11-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Infection and Public Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jiph.2024.102596\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Infection and Public Health","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.jiph.2024.102596","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Emergence of multidrug-resistant E. coli ST8346 isolates carrying three distinct plasmids with NDM-5, KPC-2, and OXA-181.
Background: E. coli ST8346 is a unique strain associated with the potential carriage of multiple carbapenemases. Three unique E. coli ST8346 isolates were identified, each concurrently harboring multiple carbapenemases, including blaNDM-5, blaKPC-2, and/or blaOXA-181. This study aimed to characterize the genetic and plasmid structures of these isolates to understand their transmission and resistance mechanisms.
Methods: Antibiotic resistance profiles, genetic relatedness, and plasmid structures were determined using antibiotic susceptibility testing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), S1 nuclease PFGE, and long-read sequencing.
Results: All the strains were carbapenem resistant. PFGE revealed close genetic relationships among the isolates, despite the lack of geographical or epidemiological connections. blaNDM-5, blaKPC-2, and blaOXA-181 were located on separate plasmids. Plasmids harboring blaNDM-5 showed genetic similarities to blaNDM-5-bearing IncF plasmids in the United Kingdom. The IncA/C plasmids harboring blaKPC-2 had identical sequences resembling a plasmid from a K. pneumoniae strain in Taiwan, except for the blaKPC-2 region, which matched a strain from China, indicating a hybrid plasmid.
Conclusion: This study is the first to identify and characterize the coexistence of blaNDM-5, blaKPC-2, and blaOXA-181 in E. coli ST8346 isolates. The spread appears to be due to independent acquisition events. We identified the putative origins of these plasmids and detected a possible recombination event in a novel IncA/C plasmid. These findings emphasize the importance of ongoing surveillance and further investigations.
期刊介绍：
The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other.
The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners.
It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.
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