Optimization and kinetic study of glucose production from agricultural waste

Agricultural waste consisting of sugarcane bagasse (SB), cassava peels (RH) and rice husk (RH) were characterized in this study by EDXRF, SEM/EDX, XRD, FTIR, proximate and ultimate analyses. The SB waste showed the highest potential for glucose yield production and was utilized in a Response Surface Methodology (RSM) optimization and kinetic study of enzymatic hydrolysis using isolated Aspergillus niger. An optimized glucose yield of maximum concentration of 92.522 mg/mL was achieved under specific conditions such as time (55.3 min), pH (4.4) and biomass (0.89g). In the kinetic study, the enzymic hydrolysis obeyed the Michaelis-Menten kinetic model, the V_max value was measured at 1.06 mg/mL/h, indicating the maximum rate of reaction achievable under the given experimental conditions. Additionally, the K_M (0.28), representing the substrate concentration at which the reaction rate is half of V_max. This study demonstrates the potential of agricultural waste, as efficient biofuel feedstocks, achieving high glucose yields through optimized enzymatic hydrolysis, crucial for advancing sustainable bioenergy production.