Rui Xu , Ai-Pei Li , Xin Tan , Xue Tang , Xiao-Ping He , Li-Xia Wang , Juan-Juan Kang , Sheng-Hong Li , Yan Liu
{"title":"广藿香精油通过JNK-1/DAF-16延长秀丽隐杆线虫的寿命和健康期","authors":"Rui Xu , Ai-Pei Li , Xin Tan , Xue Tang , Xiao-Ping He , Li-Xia Wang , Juan-Juan Kang , Sheng-Hong Li , Yan Liu","doi":"10.1016/j.lfs.2024.123270","DOIUrl":null,"url":null,"abstract":"<div><h3>Aims</h3><div>Patchouli essential oil (PEO) is the major active ingredient of a famous medicinal plant <em>Pogostemon cablin</em> (Blanco) Benth. This study aims to investigate the anti-aging activities of PEO and its major component, and elucidate the underlying molecular mechanisms.</div></div><div><h3>Main methods</h3><div>The anti-aging activities of PEO and its main component patchouli alcohol (PA) were investigated by examining the lifespan, senescence associated indicators as well as stress resistance of <em>Caenorhabditis elegans</em>. RNA-Sequencing was performed to analyze differentially expressed genes and the enrichments of GO and KEGG pathways in nematodes treated with PEO. The potential anti-aging target was predicted using a network pharmacology method and molecular docking. The underlying mechanism of senescence-delaying action was explored using <em>C. elegans</em> mutants and GFP transgenic strains.</div></div><div><h3>Key findings</h3><div>PEO modulated lifespan and healthspan extension of worms, ameliorated the senescence characterizations, and increased the survival in stress resistance assays. PEO reduced spawning, lipid accumulation and reactive oxygen species (ROS) levels of nematodes. The levels of anti-oxidative genes and proteins were obviously upregulated after PEO treatment. Moreover, PA was identified to be an ingredient for PEO-mediated nematode longevity. The JNK-1/DAF-16 signaling pathway played a critical role in PEO/PA-mediated longevity.</div></div><div><h3>Significance</h3><div>The findings revealed that PEO and its major component PA showed significant anti-aging activity through modulating the JNK-1/DAF-16 signaling pathway, which provides a promising strategy to treat aging and age-related diseases.</div></div>","PeriodicalId":18122,"journal":{"name":"Life sciences","volume":"360 ","pages":"Article 123270"},"PeriodicalIF":5.2000,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Patchouli essential oil extends the lifespan and healthspan of Caenorhabditis elegans through JNK-1/DAF-16\",\"authors\":\"Rui Xu , Ai-Pei Li , Xin Tan , Xue Tang , Xiao-Ping He , Li-Xia Wang , Juan-Juan Kang , Sheng-Hong Li , Yan Liu\",\"doi\":\"10.1016/j.lfs.2024.123270\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Aims</h3><div>Patchouli essential oil (PEO) is the major active ingredient of a famous medicinal plant <em>Pogostemon cablin</em> (Blanco) Benth. This study aims to investigate the anti-aging activities of PEO and its major component, and elucidate the underlying molecular mechanisms.</div></div><div><h3>Main methods</h3><div>The anti-aging activities of PEO and its main component patchouli alcohol (PA) were investigated by examining the lifespan, senescence associated indicators as well as stress resistance of <em>Caenorhabditis elegans</em>. RNA-Sequencing was performed to analyze differentially expressed genes and the enrichments of GO and KEGG pathways in nematodes treated with PEO. The potential anti-aging target was predicted using a network pharmacology method and molecular docking. The underlying mechanism of senescence-delaying action was explored using <em>C. elegans</em> mutants and GFP transgenic strains.</div></div><div><h3>Key findings</h3><div>PEO modulated lifespan and healthspan extension of worms, ameliorated the senescence characterizations, and increased the survival in stress resistance assays. PEO reduced spawning, lipid accumulation and reactive oxygen species (ROS) levels of nematodes. The levels of anti-oxidative genes and proteins were obviously upregulated after PEO treatment. Moreover, PA was identified to be an ingredient for PEO-mediated nematode longevity. The JNK-1/DAF-16 signaling pathway played a critical role in PEO/PA-mediated longevity.</div></div><div><h3>Significance</h3><div>The findings revealed that PEO and its major component PA showed significant anti-aging activity through modulating the JNK-1/DAF-16 signaling pathway, which provides a promising strategy to treat aging and age-related diseases.</div></div>\",\"PeriodicalId\":18122,\"journal\":{\"name\":\"Life sciences\",\"volume\":\"360 \",\"pages\":\"Article 123270\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2024-11-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Life sciences\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0024320524008609\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Life sciences","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0024320524008609","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
Patchouli essential oil extends the lifespan and healthspan of Caenorhabditis elegans through JNK-1/DAF-16
Aims
Patchouli essential oil (PEO) is the major active ingredient of a famous medicinal plant Pogostemon cablin (Blanco) Benth. This study aims to investigate the anti-aging activities of PEO and its major component, and elucidate the underlying molecular mechanisms.
Main methods
The anti-aging activities of PEO and its main component patchouli alcohol (PA) were investigated by examining the lifespan, senescence associated indicators as well as stress resistance of Caenorhabditis elegans. RNA-Sequencing was performed to analyze differentially expressed genes and the enrichments of GO and KEGG pathways in nematodes treated with PEO. The potential anti-aging target was predicted using a network pharmacology method and molecular docking. The underlying mechanism of senescence-delaying action was explored using C. elegans mutants and GFP transgenic strains.
Key findings
PEO modulated lifespan and healthspan extension of worms, ameliorated the senescence characterizations, and increased the survival in stress resistance assays. PEO reduced spawning, lipid accumulation and reactive oxygen species (ROS) levels of nematodes. The levels of anti-oxidative genes and proteins were obviously upregulated after PEO treatment. Moreover, PA was identified to be an ingredient for PEO-mediated nematode longevity. The JNK-1/DAF-16 signaling pathway played a critical role in PEO/PA-mediated longevity.
Significance
The findings revealed that PEO and its major component PA showed significant anti-aging activity through modulating the JNK-1/DAF-16 signaling pathway, which provides a promising strategy to treat aging and age-related diseases.
期刊介绍：
Life Sciences is an international journal publishing articles that emphasize the molecular, cellular, and functional basis of therapy. The journal emphasizes the understanding of mechanism that is relevant to all aspects of human disease and translation to patients. All articles are rigorously reviewed.
The Journal favors publication of full-length papers where modern scientific technologies are used to explain molecular, cellular and physiological mechanisms. Articles that merely report observations are rarely accepted. Recommendations from the Declaration of Helsinki or NIH guidelines for care and use of laboratory animals must be adhered to. Articles should be written at a level accessible to readers who are non-specialists in the topic of the article themselves, but who are interested in the research. The Journal welcomes reviews on topics of wide interest to investigators in the life sciences. We particularly encourage submission of brief, focused reviews containing high-quality artwork and require the use of mechanistic summary diagrams.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
