使用适配的 3D 打印机在凝胶电泳中装载样品。

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS Analytical biochemistry Pub Date : 2024-11-23 DOI:10.1016/j.ab.2024.115721

Tao Tao , Alexey Kopyl , Yuki Yew , Fawaz El-Dani , Hassan Ali Abid , Thomas Hiscox , Oi Wah Liew , Tuck Wah Ng

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引用次数: 0

摘要

在凝胶电泳过程中，如果将样品分装得过高或过低，都会导致电泳结果不理想。在这里，我们发现，配备光学传感器的改装 3D 打印机液体处理装置可实现垂直样品输送定位，其标准偏差与平均值之比为 0.008。这说明使用自动化技术可以实现高精度和可重复性的结果，而且成本低、技术知识需求少。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Sample loading in gel electrophoresis using adapted 3D printers

In gel electrophoresis, samples that are dispensed too high above or too low into the wells result in sub-optimal outcomes. Here, an adapted 3D printer liquid handler equipped with an optical sensor was found to attain vertical sample delivery positionings at a standard deviation over mean ratio of 0.008. This illustrated high accuracy and repeatability outcomes achieved using automation that is cost effective and low in technical knowledge demand.

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.