[分枝杆菌抗原 85B 抑制霍奇金淋巴瘤细胞自噬并促进其凋亡的机制］

Q3 Medicine 中国当代儿科杂志 Pub Date : 2024-11-15 DOI:10.7499/j.issn.1008-8830.2404153

Yong-Feng Cheng, Yi-Ping Shen, Xue-Mei Wang, Dan-Lu Li, Chun-Yan Fan, Gulibaha Maimaiti, Mei Yan

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Human Hodgkin lymphoma cells (HDLM-2) were divided into the HDLM-2 group and the HDLM-2+Ag85B groups (with Ag85B concentrations of 0.5, 1, 2, and 4 μg/mL). The CCK8 method was used to measure HDLM-2 cell proliferation; qRT-PCR was employed to detect the expression of LC3, P62, Beclin-1, Akt, and mTOR mRNA in cells. An apoptosis kit was used to detect cell apoptosis.Results: The positive expression of LC3 and Beclin-1 in the HL group were higher than those in the control group (P<0.05), while the positive expression of P62 was lower than that in the control group (P<0.05). In stages III-IV compared to stages I-II, the positive expression of LC3 and Beclin-1 increased, while the positive expression of P62 decreased (P<0.05). 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引用次数: 0

摘要

目的研究分枝杆菌抗原85B（Ag85B）抑制霍奇金淋巴瘤（HL）细胞自噬并促进其凋亡的机制：方法：回顾性收集新疆医科大学第一附属医院收治的80例霍奇金淋巴瘤患儿和30例反应性淋巴结病患儿（对照组）的临床资料和病理组织切片。免疫组化分析评估了HL组和对照组病理组织中微管相关蛋白1轻链3（LC3）、序列组1（P62/SQSTM1）和Beclin-1的表达。将人霍奇金淋巴瘤细胞（HDLM-2）分为 HDLM-2 组和 HDLM-2+Ag85B 组（Ag85B 浓度分别为 0.5、1、2 和 4 μg/mL）。采用 CCK8 法检测 HDLM-2 细胞的增殖情况；采用 qRT-PCR 法检测细胞中 LC3、P62、Beclin-1、Akt 和 mTOR mRNA 的表达情况。使用细胞凋亡试剂盒检测细胞凋亡：结果：HL 组中 LC3 和 Beclin-1 的阳性表达高于对照组（PPPPConclusions：HL患儿的自噬功能增强，并随疾病阶段的增加而增强。Ag85B能抑制HL肿瘤细胞的增殖和自噬，促进细胞凋亡，这可能与激活PI3K/Akt/mTOR通路有关。

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[Mechanism by which mycobacterial antigen 85B inhibits autophagy and promotes apoptosis in Hodgkin lymphoma cells].

Objectives: To investigate the mechanism by which mycobacterial antigen 85B (Ag85B) inhibits autophagy and promotes apoptosis in Hodgkin lymphoma (HL) cells.

Methods: The clinical data and pathological tissue slides were retrospectively collected from 80 HL children and 30 children with reactive lymphadenopathy (control group) treated at the First Affiliated Hospital of Xinjiang Medical University. Immunohistochemical analysis was performed to assess the expression of microtubule-associated protein 1 light chain 3 (LC3), sequestosome 1 (P62/SQSTM1), and Beclin-1 in the pathological tissues of HL and control groups. Human Hodgkin lymphoma cells (HDLM-2) were divided into the HDLM-2 group and the HDLM-2+Ag85B groups (with Ag85B concentrations of 0.5, 1, 2, and 4 μg/mL). The CCK8 method was used to measure HDLM-2 cell proliferation; qRT-PCR was employed to detect the expression of LC3, P62, Beclin-1, Akt, and mTOR mRNA in cells. An apoptosis kit was used to detect cell apoptosis.

Results: The positive expression of LC3 and Beclin-1 in the HL group were higher than those in the control group (P<0.05), while the positive expression of P62 was lower than that in the control group (P<0.05). In stages III-IV compared to stages I-II, the positive expression of LC3 and Beclin-1 increased, while the positive expression of P62 decreased (P<0.05). Cell experiment results showed that the HDLM-2+Ag85B group had suppressed cell proliferation compared to the HDLM-2 group, with decreased mRNA expression of LC3 and Beclin-1, and increased mRNA expression of P62, PI3K, Akt, and mTOR, leading to increased cell apoptosis. Notably, when Ag85B was at a concentration of 2 μg/mL, it had the strongest effect on HDLM-2 cells after 24 hours (P<0.05).

Conclusions: Autophagy is enhanced in children with HL and increases with disease stage. Ag85B can inhibit the proliferation and autophagy of HL tumor cells and promote apoptosis, possibly related to the activation of the PI3K/Akt/mTOR pathway.

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来源期刊

中国当代儿科杂志 Medicine-Pediatrics, Perinatology and Child Health

CiteScore

1.50

自引率

0.00%

发文量

5006

期刊介绍： The Chinese Journal of Contemporary Pediatrics (CJCP) is a peer-reviewed open access periodical in the field of pediatrics that is sponsored by the Central South University/Xiangya Hospital of Central South University and under the auspices of the Ministry of Education of China. It is cited as a source in the scientific and technological papers of Chinese journals, the Chinese Science Citation Database (CSCD), and is one of the core Chinese periodicals in the Peking University Library. CJCP has been indexed by MEDLINE/PubMed/PMC of the American National Library, American Chemical Abstracts (CA), Holland Medical Abstracts (EM), Western Pacific Region Index Medicus (WPRIM), Scopus and EBSCO. It is a monthly periodical published on the 15th of every month, and is distributed both at home and overseas. The Chinese series publication number is CN 43-1301/R；ISSN 1008-8830. The tenet of CJCP is to “reflect the latest advances and be open to the world”. The periodical reports the most recent advances in the contemporary pediatric field. The majority of the readership is pediatric doctors and researchers.