Risk assessment of dietary exposure due to Cyromazine and Melamine residues in poultry eggs from layer farms of Haryana, India

The practice of using Cyromazine (CYR) as feed additive larvicide at layer farms leads to occurrence of residues of CYR and its metabolite Melamine (MEL) in eggs and can cause public health problem. Therefore, the present research work was carried out to estimate the dietary exposure risk to consumers in Haryana, India where CYR is routinely used at layer farms as a measure of fly control. For this purpose, 110 egg samples were collected from layer farms located in selected districts of Haryana (Panchkula, Hisar, Ambala, Sirsa and Panipat). Samples were analyzed using High Pressure Liquid Chromatography (HPLC-UV). CYR concentration was found to be above LOQ in 25 samples. The mean concentration of the CYR was found to be 42.71 µg kg⁻¹ with a range of 16.88 -112.61 µg kg⁻¹. MEL was not detected in any of the sample analyzed. Estimates of dietary exposure of CYR was found to be 33.40 µg per kg body weight per day. The results indicated that poultry farmers are using CYR as feed additive in layer birds for the purpose of fly control by following good agricultural practices and there is no risk of dietary exposure of CYR and MEL through consumption of eggs to the consumers.