利用分子网络和抗ra - fls活性靶向分离木脂素和三萜。

IF 3.2 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Phytochemistry Pub Date : 2024-11-28 DOI:10.1016/j.phytochem.2024.114341
Shi-qi Liu , Qing-ling Xie , Ya-si Deng , Ling Liang , Han-wen Yuan , Bin Li , Huang-he Yu , Xing Tian , Yi-xing Qiu , Guy Paulin Kemayou Mouthe , Nuzhat Shehla , Ye Zhang , Ze-bo Cai , Wei Wang , Yu-pei Yang
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引用次数: 0

摘要

本研究从鸭尾草(Kadsura coccinea, Lem)的根中分离得到6个化合物(4个三萜化合物命名为heilaohutriterpenes A-D, 2个木脂素化合物命名为heilaohusuins F和G)和21个已知化合物。a.c.史密斯的分子网络指导。采用HR-ESI-MS, 1D, 2D-NMR分析,NMR计算和电子圆二色性(ECD)计算相结合的方法确定其结构。此外,分离的化合物抑制类风湿性关节炎-成纤维样滑膜(RA-FLS)细胞增殖的能力在体外进行了评估。海螺三萜类化合物B(2)、海螺三萜类化合物D(4)、瓢虫苷类化合物B(7)和仙桃素类化合物H(24)对RA-FLS细胞具有显著的抑制作用,IC50值分别为9.57±0.84 μM、16.22±1.71 μM、3.08±1.59 μM和19.09±2.42 μM。western blotting分析显示,化合物2下调P-NF-κB p65水平,上调Bax和i -κB α水平。综上所述,化合物2通过抑制NF-κB通路促进RA-FLS细胞凋亡。综上所述,本研究有助于提高球菌衍生化合物的结构多样性,为进一步开展抗ra相关研究奠定基础。
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Targeted isolation of lignans and triterpenoids from kadsura coccinea by molecular networking and anti-RA-FLS activity
In this study, six compounds (four triterpenoids named heilaohutriterpenes A-D and two lignans name heilaohusuins F and G) together with 21 known compounds were isolated from roots of Kadsura coccinea (Lem.) A. C. Smith guided by molecular networking. Their structures were determined using a combination of HR-ESI-MS, 1D, 2D-NMR anatysis, NMR calculation, and electronic circular dichroism (ECD) calculations. Moreover, the ability of the isolated compounds to inhibit the proliferation of rheumatoid arthritis-fibroblastoid synovial (RA-FLS) cells was evaluated in vitro. Heilaohutriterpene B (2), heilaohutriterpene D (4), coccinone B (7), and kadsuralignan H (24) demonstrated significant inhibitory activities against RA-FLS cells, with IC50 values of 9.57 ± 0.84, 16.22 ± 1.71, 3.08 ± 1.59, and 19.09 ± 2.42 μM, respectively. Meanwhile, western blotting analysis revealed that compound 2 down-regulated the level of P–NF-κB p65 and up-regulated that of Bax and IκBα. These results collectively suggest that compound 2 promoted the apoptosis of RA-FLS cells by inhibiting the NF-κB pathway. Taken together, this study contributed to the structural diversity of compounds derived from K. coccinea and lays a basis for further anti-RA-related studies.
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来源期刊
Phytochemistry
Phytochemistry 生物-植物科学
CiteScore
6.40
自引率
7.90%
发文量
443
审稿时长
39 days
期刊介绍: Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.
期刊最新文献
Editorial Board Anti-inflammatory sesquiterpenoids from Chloranthus japonicus Megastigmane glycosides from Heterosmilax yunnanensis and their neuroprotective activity Undescribed alkaloids, peptides and polyketides from marine sediment-derived fungus Aspergillus terreus PPS1. Editorial Board
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