Shi-qi Liu , Qing-ling Xie , Ya-si Deng , Ling Liang , Han-wen Yuan , Bin Li , Huang-he Yu , Xing Tian , Yi-xing Qiu , Guy Paulin Kemayou Mouthe , Nuzhat Shehla , Ye Zhang , Ze-bo Cai , Wei Wang , Yu-pei Yang
{"title":"利用分子网络和抗ra - fls活性靶向分离木脂素和三萜。","authors":"Shi-qi Liu , Qing-ling Xie , Ya-si Deng , Ling Liang , Han-wen Yuan , Bin Li , Huang-he Yu , Xing Tian , Yi-xing Qiu , Guy Paulin Kemayou Mouthe , Nuzhat Shehla , Ye Zhang , Ze-bo Cai , Wei Wang , Yu-pei Yang","doi":"10.1016/j.phytochem.2024.114341","DOIUrl":null,"url":null,"abstract":"<div><div>In this study, six compounds (four triterpenoids named heilaohutriterpenes A-D and two lignans name heilaohusuins F and G) together with 21 known compounds were isolated from roots of <em>Kadsura coccinea</em> (Lem.) A. C. Smith guided by molecular networking. Their structures were determined using a combination of HR-ESI-MS, 1D, 2D-NMR anatysis, NMR calculation, and electronic circular dichroism (ECD) calculations. Moreover, the ability of the isolated compounds to inhibit the proliferation of rheumatoid arthritis-fibroblastoid synovial (RA-FLS) cells was evaluated <em>in vitro</em>. Heilaohutriterpene B (<strong>2</strong>), heilaohutriterpene D (<strong>4</strong>), coccinone B (<strong>7</strong>), and kadsuralignan H (<strong>24</strong>) demonstrated significant inhibitory activities against RA-FLS cells, with IC<sub>50</sub> values of 9.57 ± 0.84, 16.22 ± 1.71, 3.08 ± 1.59, and 19.09 ± 2.42 μM, respectively. Meanwhile, western blotting analysis revealed that compound <strong>2</strong> down-regulated the level of <em>P</em>–NF-κB p65 and up-regulated that of Bax and IκBα. These results collectively suggest that compound <strong>2</strong> promoted the apoptosis of RA-FLS cells by inhibiting the NF-κB pathway. Taken together, this study contributed to the structural diversity of compounds derived from <em>K. coccinea</em> and lays a basis for further anti-RA-related studies.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"231 ","pages":"Article 114341"},"PeriodicalIF":3.2000,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Targeted isolation of lignans and triterpenoids from kadsura coccinea by molecular networking and anti-RA-FLS activity\",\"authors\":\"Shi-qi Liu , Qing-ling Xie , Ya-si Deng , Ling Liang , Han-wen Yuan , Bin Li , Huang-he Yu , Xing Tian , Yi-xing Qiu , Guy Paulin Kemayou Mouthe , Nuzhat Shehla , Ye Zhang , Ze-bo Cai , Wei Wang , Yu-pei Yang\",\"doi\":\"10.1016/j.phytochem.2024.114341\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>In this study, six compounds (four triterpenoids named heilaohutriterpenes A-D and two lignans name heilaohusuins F and G) together with 21 known compounds were isolated from roots of <em>Kadsura coccinea</em> (Lem.) A. C. Smith guided by molecular networking. Their structures were determined using a combination of HR-ESI-MS, 1D, 2D-NMR anatysis, NMR calculation, and electronic circular dichroism (ECD) calculations. Moreover, the ability of the isolated compounds to inhibit the proliferation of rheumatoid arthritis-fibroblastoid synovial (RA-FLS) cells was evaluated <em>in vitro</em>. Heilaohutriterpene B (<strong>2</strong>), heilaohutriterpene D (<strong>4</strong>), coccinone B (<strong>7</strong>), and kadsuralignan H (<strong>24</strong>) demonstrated significant inhibitory activities against RA-FLS cells, with IC<sub>50</sub> values of 9.57 ± 0.84, 16.22 ± 1.71, 3.08 ± 1.59, and 19.09 ± 2.42 μM, respectively. Meanwhile, western blotting analysis revealed that compound <strong>2</strong> down-regulated the level of <em>P</em>–NF-κB p65 and up-regulated that of Bax and IκBα. These results collectively suggest that compound <strong>2</strong> promoted the apoptosis of RA-FLS cells by inhibiting the NF-κB pathway. Taken together, this study contributed to the structural diversity of compounds derived from <em>K. coccinea</em> and lays a basis for further anti-RA-related studies.</div></div>\",\"PeriodicalId\":20170,\"journal\":{\"name\":\"Phytochemistry\",\"volume\":\"231 \",\"pages\":\"Article 114341\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2024-11-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0031942224003789\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0031942224003789","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Targeted isolation of lignans and triterpenoids from kadsura coccinea by molecular networking and anti-RA-FLS activity
In this study, six compounds (four triterpenoids named heilaohutriterpenes A-D and two lignans name heilaohusuins F and G) together with 21 known compounds were isolated from roots of Kadsura coccinea (Lem.) A. C. Smith guided by molecular networking. Their structures were determined using a combination of HR-ESI-MS, 1D, 2D-NMR anatysis, NMR calculation, and electronic circular dichroism (ECD) calculations. Moreover, the ability of the isolated compounds to inhibit the proliferation of rheumatoid arthritis-fibroblastoid synovial (RA-FLS) cells was evaluated in vitro. Heilaohutriterpene B (2), heilaohutriterpene D (4), coccinone B (7), and kadsuralignan H (24) demonstrated significant inhibitory activities against RA-FLS cells, with IC50 values of 9.57 ± 0.84, 16.22 ± 1.71, 3.08 ± 1.59, and 19.09 ± 2.42 μM, respectively. Meanwhile, western blotting analysis revealed that compound 2 down-regulated the level of P–NF-κB p65 and up-regulated that of Bax and IκBα. These results collectively suggest that compound 2 promoted the apoptosis of RA-FLS cells by inhibiting the NF-κB pathway. Taken together, this study contributed to the structural diversity of compounds derived from K. coccinea and lays a basis for further anti-RA-related studies.
期刊介绍:
Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.