奶牛中流行H5N1流感病毒的快速安全中和试验

IF 4.3 4区医学 Q1 INFECTIOUS DISEASES Influenza and Other Respiratory Viruses Pub Date : 2024-12-02 DOI:10.1111/irv.70048

Kei Miyakawa, Makoto Ota, Kaori Sano, Fumitaka Momose, Takashi Okura, Noriko Kishida, Tomoko Arita, Yasushi Suzuki, Masayuki Shirakura, Hideki Asanuma, Shinji Watanabe, Akihide Ryo, Hideki Hasegawa

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引用次数: 0

摘要

需要对高致病性禽流感病毒进行快速和安全的中和试验，包括最近在奶牛中发现的一种进化枝2.3.4.4b H5N1亚型。在这里，我们报告了一种使用发光病毒样颗粒的中和试验。该分析具有较低的生物安全要求，并提供比传统方法更大的动态范围。我们应用该技术评价了2.3.4.4b支候选疫苗病毒（CVVs）诱导的中和抗体与牛源H5N1病毒的交叉反应性。我们的研究结果表明，这些cvv与牛源性H5N1病毒具有相同的抗原特征，这表明使用这些cvv开发的疫苗具有潜在功效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Rapid and Safe Neutralization Assay for Circulating H5N1 Influenza Virus in Dairy Cows

Rapid and safe neutralization assays are required for highly pathogenic avian influenza viruses, including a clade 2.3.4.4b H5N1 subtype recently found in cows. Here, we report a neutralization assay using luminescent virus-like particles. This assay has lower biosafety requirements and provides a larger dynamic range than conventional methods. We applied this technique to evaluate the cross-reactivity of neutralizing antibodies induced by clade 2.3.4.4b candidate vaccine viruses (CVVs) with the cow-derived H5N1 virus. Our findings indicate that these CVVs share antigenic characteristics with the cow-derived H5N1 virus, suggesting the potential efficacy of vaccines developed using these CVVs.

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来源期刊

Influenza and Other Respiratory Viruses 医学-病毒学

CiteScore

7.20

自引率

4.50%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Influenza and Other Respiratory Viruses is the official journal of the International Society of Influenza and Other Respiratory Virus Diseases - an independent scientific professional society - dedicated to promoting the prevention, detection, treatment, and control of influenza and other respiratory virus diseases. Influenza and Other Respiratory Viruses is an Open Access journal. Copyright on any research article published by Influenza and Other Respiratory Viruses is retained by the author(s). Authors grant Wiley a license to publish the article and identify itself as the original publisher. Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors, citation details and publisher are identified.