Huihuang Li , Yue Yang , Fan Yang, Xinguo Bao, Chengfu Pan, Weilong Lin, Lianjie Lai, Weimin Lin, Ruiyi Lin
{"title":"莆田黑鸭血液生化指标测定及蛋品质相关候选基因CDH5的研究。","authors":"Huihuang Li , Yue Yang , Fan Yang, Xinguo Bao, Chengfu Pan, Weilong Lin, Lianjie Lai, Weimin Lin, Ruiyi Lin","doi":"10.1016/j.gene.2024.149142","DOIUrl":null,"url":null,"abstract":"<div><div>Improving egg quality and enhancing production efficiency are essential goals in poultry breeding. <em>CDH5</em> encodes a cadherin involved in Ca<sup>2+</sup> transport in endothelial cells. The role of <em>CDH5</em> in regulating duck egg quality and its mechanisms affecting Ca<sup>2+</sup> concentrations in duck uterine epithelial cells remains unclear. This study evaluated egg quality traits of the Putian black duck and conducted an association analysis with blood biochemical indices and single nucleotide polymorphisms in <em>CDH5</em>. Additionally, we constructed a <em>CDH5</em> overexpression vector and synthesized specific siRNAs for transfection into Putian black duck uterine epithelial cells to assess Ca<sup>2+</sup> concentrations. Our results revealed a significant association between egg quality and five novel SNPs in <em>CDH5</em>, along with various blood biochemical indices. Further experiments demonstrated that <em>CDH5</em> overexpression and knockdown reduced Ca<sup>2+</sup> concentrations in the uterine epithelial cells of Putian black ducks.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"937 ","pages":"Article 149142"},"PeriodicalIF":2.6000,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Determination of blood biochemical indices and research of egg quality-related candidate gene CDH5 in Putian black duck\",\"authors\":\"Huihuang Li , Yue Yang , Fan Yang, Xinguo Bao, Chengfu Pan, Weilong Lin, Lianjie Lai, Weimin Lin, Ruiyi Lin\",\"doi\":\"10.1016/j.gene.2024.149142\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Improving egg quality and enhancing production efficiency are essential goals in poultry breeding. <em>CDH5</em> encodes a cadherin involved in Ca<sup>2+</sup> transport in endothelial cells. The role of <em>CDH5</em> in regulating duck egg quality and its mechanisms affecting Ca<sup>2+</sup> concentrations in duck uterine epithelial cells remains unclear. This study evaluated egg quality traits of the Putian black duck and conducted an association analysis with blood biochemical indices and single nucleotide polymorphisms in <em>CDH5</em>. Additionally, we constructed a <em>CDH5</em> overexpression vector and synthesized specific siRNAs for transfection into Putian black duck uterine epithelial cells to assess Ca<sup>2+</sup> concentrations. Our results revealed a significant association between egg quality and five novel SNPs in <em>CDH5</em>, along with various blood biochemical indices. Further experiments demonstrated that <em>CDH5</em> overexpression and knockdown reduced Ca<sup>2+</sup> concentrations in the uterine epithelial cells of Putian black ducks.</div></div>\",\"PeriodicalId\":12499,\"journal\":{\"name\":\"Gene\",\"volume\":\"937 \",\"pages\":\"Article 149142\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-12-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0378111924010230\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0378111924010230","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Determination of blood biochemical indices and research of egg quality-related candidate gene CDH5 in Putian black duck
Improving egg quality and enhancing production efficiency are essential goals in poultry breeding. CDH5 encodes a cadherin involved in Ca2+ transport in endothelial cells. The role of CDH5 in regulating duck egg quality and its mechanisms affecting Ca2+ concentrations in duck uterine epithelial cells remains unclear. This study evaluated egg quality traits of the Putian black duck and conducted an association analysis with blood biochemical indices and single nucleotide polymorphisms in CDH5. Additionally, we constructed a CDH5 overexpression vector and synthesized specific siRNAs for transfection into Putian black duck uterine epithelial cells to assess Ca2+ concentrations. Our results revealed a significant association between egg quality and five novel SNPs in CDH5, along with various blood biochemical indices. Further experiments demonstrated that CDH5 overexpression and knockdown reduced Ca2+ concentrations in the uterine epithelial cells of Putian black ducks.
期刊介绍：
Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.
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