Pristionchus – Beetle associations: Towards a new natural history

The free-living nematode Pristionchus pacificus has been established as a model system in integrative evolutionary biology by combining laboratory studies with field work and evolutionary biology. Multiple genetic, molecular and experimental tools and a collection of more than 2,500 P. pacificus strains and more than 50 Pristionchus species, which are available as living cultures or frozen stock collections, support research on various life history traits. Species of Pristionchus exhibit a number of complex traits unknown from Caenorhabditis elegans and most other free-living nematodes. First, P. pacificus can form two alternative mouth forms, an example of developmental plasticity that is increasingly studied to investigate the role of plasticity as a facilitator of evolutionary novelty. More than a decade of work has identified associated genetic and epigenetic mechanisms and revealed the evolutionary and ecological significance of feeding structure plasticity. Second, one of the two mouth morphs results in predatory behavior against other nematodes and is currently used to investigate the neurobiology of predation. Third, potential predation results in the risk of cannibalism among conspecifics. Strikingly, Pristionchus nematodes have developed a self-recognition system that allows the distinction of self (kin) and non-self. Given all these organismal features, this nematode has recently been considered a key example for research towards a new natural history (West-Eberhard, 2024). Here, I summarize recent work on Pristionchus with a focus on a ‘new natural history’. In addition, I review some recent studies that indicate an interaction of Pristionchus with EPNs that was suggested based on various surveys in different ecological habitats.