Kan Yu , Minjia Zhu , Zixiang Dai , Qinrou Zhang , Le Xiao , Xinyi Li , Jialiang Dai , Zihan Jia , Jingyi Li , Yuxing Bai , Ke Zhang
{"title":"二甲双胍用于牙周和牙根再生的牙树脂促进人牙周韧带干细胞成骨和骨质分化。","authors":"Kan Yu , Minjia Zhu , Zixiang Dai , Qinrou Zhang , Le Xiao , Xinyi Li , Jialiang Dai , Zihan Jia , Jingyi Li , Yuxing Bai , Ke Zhang","doi":"10.1016/j.jdent.2024.105507","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>This study developed a novel dental resin incorporating metformin to repair root caries and periodontitis defects. The objectives were to: (1) Develop a novel dental resin with metformin release to fulfill the clinical requirements for mechanical properties; and (2) investigate the metformin release pattern and the effects on osteogenic and cementogenic differentiation of human periodontal ligament stem cells (hPDLSCs).</div></div><div><h3>Methods</h3><div>Resin specimens with different concentrations of metformin were fabricated. Metformin release was measured using high-performance liquid chromatography. Cellular growth and proliferation on resin were assessed. Alizarin red S staining and an alkaline phosphatase (ALP) activity were determined. Quantitative real-time reverse transcription PCR was employed to determine osteogenic and cementogenic differentiation.</div></div><div><h3>Results</h3><div>The resin with 7.5 % metformin had mechanical properties comparable to those of a commercial control, thus satisfying the clinical requirements (mean ± SD; <em>n =</em> 10). hPDLSCs showed robust growth and proliferation on resin. At 14 days, the number of hPDLSCs increased by four-fold (<em>p ></em> 0.1). ALP activity in the metformin group was 1.8 times higher than that of control (<em>p <</em> 0.05). At 21 days, the metformin group showed a significant increase in mineral synthesis. Metformin group had cementogenic and osteogenic differentiation values that were 2.5 and 2.3 times higher, respectively, than control without metformin (<em>p <</em> 0.05).</div></div><div><h3>Significance</h3><div>A metformin-resin was formulated that greatly promoted both cementogenic and osteogenic differentiation for the first time. This resin was mechanically strong and supported hPDLSC adhesion, growth and proliferation. This resin substantially enhanced hPDLSC osteogenic and cementogenic differentiation, yielding mineral synthesis of hPDLSCs more than 2-fold that of control without metformin. This new metformin-resin shows promise for the restoration of tooth root caries and the regeneration of periodontal tissues including alveolar bone and cementum.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"153 ","pages":"Article 105507"},"PeriodicalIF":4.8000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dental resin for periodontal and tooth root regeneration via metformin to enhance osteogenic and cementogenic differentiation of human periodontal ligament stem cells\",\"authors\":\"Kan Yu , Minjia Zhu , Zixiang Dai , Qinrou Zhang , Le Xiao , Xinyi Li , Jialiang Dai , Zihan Jia , Jingyi Li , Yuxing Bai , Ke Zhang\",\"doi\":\"10.1016/j.jdent.2024.105507\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><div>This study developed a novel dental resin incorporating metformin to repair root caries and periodontitis defects. The objectives were to: (1) Develop a novel dental resin with metformin release to fulfill the clinical requirements for mechanical properties; and (2) investigate the metformin release pattern and the effects on osteogenic and cementogenic differentiation of human periodontal ligament stem cells (hPDLSCs).</div></div><div><h3>Methods</h3><div>Resin specimens with different concentrations of metformin were fabricated. Metformin release was measured using high-performance liquid chromatography. Cellular growth and proliferation on resin were assessed. Alizarin red S staining and an alkaline phosphatase (ALP) activity were determined. Quantitative real-time reverse transcription PCR was employed to determine osteogenic and cementogenic differentiation.</div></div><div><h3>Results</h3><div>The resin with 7.5 % metformin had mechanical properties comparable to those of a commercial control, thus satisfying the clinical requirements (mean ± SD; <em>n =</em> 10). hPDLSCs showed robust growth and proliferation on resin. At 14 days, the number of hPDLSCs increased by four-fold (<em>p ></em> 0.1). ALP activity in the metformin group was 1.8 times higher than that of control (<em>p <</em> 0.05). At 21 days, the metformin group showed a significant increase in mineral synthesis. Metformin group had cementogenic and osteogenic differentiation values that were 2.5 and 2.3 times higher, respectively, than control without metformin (<em>p <</em> 0.05).</div></div><div><h3>Significance</h3><div>A metformin-resin was formulated that greatly promoted both cementogenic and osteogenic differentiation for the first time. This resin was mechanically strong and supported hPDLSC adhesion, growth and proliferation. This resin substantially enhanced hPDLSC osteogenic and cementogenic differentiation, yielding mineral synthesis of hPDLSCs more than 2-fold that of control without metformin. This new metformin-resin shows promise for the restoration of tooth root caries and the regeneration of periodontal tissues including alveolar bone and cementum.</div></div>\",\"PeriodicalId\":15585,\"journal\":{\"name\":\"Journal of dentistry\",\"volume\":\"153 \",\"pages\":\"Article 105507\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of dentistry\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0300571224006766\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of dentistry","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0300571224006766","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Dental resin for periodontal and tooth root regeneration via metformin to enhance osteogenic and cementogenic differentiation of human periodontal ligament stem cells
Objectives
This study developed a novel dental resin incorporating metformin to repair root caries and periodontitis defects. The objectives were to: (1) Develop a novel dental resin with metformin release to fulfill the clinical requirements for mechanical properties; and (2) investigate the metformin release pattern and the effects on osteogenic and cementogenic differentiation of human periodontal ligament stem cells (hPDLSCs).
Methods
Resin specimens with different concentrations of metformin were fabricated. Metformin release was measured using high-performance liquid chromatography. Cellular growth and proliferation on resin were assessed. Alizarin red S staining and an alkaline phosphatase (ALP) activity were determined. Quantitative real-time reverse transcription PCR was employed to determine osteogenic and cementogenic differentiation.
Results
The resin with 7.5 % metformin had mechanical properties comparable to those of a commercial control, thus satisfying the clinical requirements (mean ± SD; n = 10). hPDLSCs showed robust growth and proliferation on resin. At 14 days, the number of hPDLSCs increased by four-fold (p > 0.1). ALP activity in the metformin group was 1.8 times higher than that of control (p < 0.05). At 21 days, the metformin group showed a significant increase in mineral synthesis. Metformin group had cementogenic and osteogenic differentiation values that were 2.5 and 2.3 times higher, respectively, than control without metformin (p < 0.05).
Significance
A metformin-resin was formulated that greatly promoted both cementogenic and osteogenic differentiation for the first time. This resin was mechanically strong and supported hPDLSC adhesion, growth and proliferation. This resin substantially enhanced hPDLSC osteogenic and cementogenic differentiation, yielding mineral synthesis of hPDLSCs more than 2-fold that of control without metformin. This new metformin-resin shows promise for the restoration of tooth root caries and the regeneration of periodontal tissues including alveolar bone and cementum.
期刊介绍：
The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review.
The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis.
Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research.
The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.
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