催乳素局部介导高催乳素血症兔的卵泡闭锁(啮齿目,金鸡科)。

IF 2.1 3区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology Pub Date : 2025-02-01 DOI:10.1016/j.cbpa.2024.111787
Santiago A. Cortasa , Luisa A. Quiroga-Baeza , Alejandro R. Schmidt , Sofía Proietto , Maria C. Corso , Alfredo D. Vitullo , Verónica B. Dorfman , Julia Halperin
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引用次数: 0

摘要

高泌乳素血症女性的不孕是由于GnRH释放失调,随后影响促性腺激素水平,最终导致无排卵。然而,除了下丘脑,催乳素受体(PRLR)也在卵巢中表达,这表明PRL在高催乳素血症病例中可能具有局部作用。我们利用野生啮齿动物平原维扎卡建立了沙匹利(SPD)诱导的高催乳素血症的实验模型,并研究了PRL药理学水平对卵泡发生和类固醇产生的影响。SPD雌性卵巢出现显著的闭锁卵泡数量,同时活卵泡总数减少,导致闭锁/活卵泡比高于对照组(CTL) (P < 0.05)。在对激素环境的敏感性方面,SPD卵巢对垂体PRL的潜在反应性显著改变,与CTL卵巢相比,PRLR的表达增加了3倍,两种促性腺激素受体的表达均增加(P < 0.05)。SPD雌性小鼠循环雌二醇(E2)值增加一倍,芳香化酶、17β-羟基类固醇脱氢酶以及E2受体、ERα和ERβ的表达均高于CTL组(P < 0.05)。我们的研究结果强烈表明,高催乳素血症依赖性卵巢功能失调至少可以部分解释为PRL的直接作用,而PRLR在卵泡和黄体中的表达升高促进了PRL的直接作用。可能这些PRL作用与促性腺激素触发的作用协同作用,最终导致类固醇生成途径的改变,卵泡发生中断和闭锁增加。
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Prolactin locally mediates follicular atresia in hyperprolactinemic vizcachas (Rodentia, Chinchillidae)
Infertility in hyperprolactinemic females is attributed to the dysregulation of GnRH release, subsequently affecting gonadotropin levels, and ultimately leading to anovulation. However, in addition to the hypothalamus, prolactin receptor (PRLR) is expressed in ovaries as well, suggesting potential local effects of PRL in cases of hyperprolactinemia. We have developed an experimental model of sulpiride (SPD)-induced hyperprolactinemia using a wild rodent, the plains vizcacha, and studied the implications of pharmacological PRL levels on folliculogenesis and steroid production. Ovaries of SPD females showed a striking number of atretic follicles along with a reduction in the collective number of viable follicles leading to a higher atretic/viable follicle ratio compared to that of control females (CTL) (P < 0.05). In terms of sensitivity to the hormonal environment, SPD ovaries substantially changed their potential responsiveness to pituitary PRL, as evidenced by the three-fold increase in PRLR expression alongside heightened expression of both gonadotropic receptors in comparison to CTL ovaries (P < 0.05). Circulating estradiol (E2) values doubled post-treatment in the SPD females, which also showed higher expressions of aromatase and 17β-hydroxysteroid dehydrogenase, along with both E2 receptors, ERα and ERβ than the CTL group (P < 0.05). Our findings strongly suggest that hyperprolactinemia-dependent dysregulation of ovarian function can be explained at least partially, by PRL direct actions facilitated by the heightened expression of PRLR in follicles and corpora lutea. Possibly these PRL actions synergize with those triggered by gonadotropic hormones ultimately leading to alteration of the steroidogenic pathway, folliculogenesis disruption and increased atresia.
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来源期刊
CiteScore
5.00
自引率
4.30%
发文量
155
审稿时长
3 months
期刊介绍: Part A: Molecular & Integrative Physiology of Comparative Biochemistry and Physiology. This journal covers molecular, cellular, integrative, and ecological physiology. Topics include bioenergetics, circulation, development, excretion, ion regulation, endocrinology, neurobiology, nutrition, respiration, and thermal biology. Study on regulatory mechanisms at any level of organization such as signal transduction and cellular interaction and control of behavior are also published.
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