Tomas Buday, Mariana Brozmanova, Janka Jakusova, Abdullah Al Owesie, Laura Sophie Ertl, Daniela Mokra, Juliana Hanusrichterova, Tatiana Burjanivova, Zuzana Biringerova, Jana Plevkova
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引用次数: 0
摘要
目的：本研究调查了在特定无病原体（SPF）条件下饲养的豚鼠与传统饲养（CON）的豚鼠的呼吸模式和免疫状况：本研究调查了在特定无病原体（SPF）条件下饲养的豚鼠与传统饲养（CON）的豚鼠的呼吸模式和免疫状态：方法：在安静呼吸和生理盐水雾化时使用全身胸透（WBP）评估呼吸模式参数。分析血液和支气管肺泡灌洗液（BALF）中的白细胞、中性粒细胞和嗜酸性粒细胞计数以及细胞因子水平（TNF-α、IL-1β、IL-4）：结果：SPF豚鼠的潮气量、呼气量、分钟量和气流参数均高于CON豚鼠。免疫分析显示，SPF豚鼠的白细胞计数和IL-4水平较低。这些结果表明，SPF 豚鼠的呼吸和免疫反应与 CON 豚鼠不同：本研究强调，影响 SPF 豚鼠呼吸模式参数的成熟过程与 CON 豚鼠的成熟过程存在显著差异。这表明，由于豚鼠的免疫和呼吸反应不同，SPF 动物在呼吸生理学研究中可能存在局限性。
Impact of microbial diversity on inflammatory cytokines and respiratory pattern measured in whole-body plethysmography in guinea pig models.
Objective: This study investigates the breathing patterns and immune status of guinea pigs raised under specific pathogen-free (SPF) conditions compared to conventionally bred (CON).
Methods: Breathing pattern parameters were assessed using whole-body plethysmography (WBP) during quiet breathing and saline nebulisation. Blood and bronchoalveolar lavage fluid (BALF) were analysed for white blood cell, neutrophil and eosinophil counts, and cytokine levels (TNF-α, IL-1β, IL-4).
Results: SPF guinea pigs exhibited higher tidal volume, expired volume, minute volume, and airflow parameters than CON guinea pigs. The immune analysis revealed lower white blood cell counts and IL-4 levels in SPF guinea pigs. These findings indicate that SPF guinea pigs have different respiratory and immune responses than CON guinea pigs.
Conclusion: The study highlights that the maturation processes affecting breathing pattern parameters in SPF guinea pigs differ significantly from those in CON guinea pigs. This suggests potential limitations of SPF animals in respiratory physiology research due to their different immune and respiratory responses.
期刊介绍：
Respiratory Physiology & Neurobiology (RESPNB) publishes original articles and invited reviews concerning physiology and pathophysiology of respiration in its broadest sense.
Although a special focus is on topics in neurobiology, high quality papers in respiratory molecular and cellular biology are also welcome, as are high-quality papers in traditional areas, such as:
-Mechanics of breathing-
Gas exchange and acid-base balance-
Respiration at rest and exercise-
Respiration in unusual conditions, like high or low pressure or changes of temperature, low ambient oxygen-
Embryonic and adult respiration-
Comparative respiratory physiology.
Papers on clinical aspects, original methods, as well as theoretical papers are also considered as long as they foster the understanding of respiratory physiology and pathophysiology.
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