含金纳米颗粒海藻酸盐/明胶可注射水凝胶用于胚胎小鼠心肌细胞移植修复。

IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Macromolecular bioscience Pub Date : 2024-12-11 DOI:10.1002/mabi.202400301
Behnam Pournemati, Hadi Tabesh, Rouhollah Mehdinavaz Aghdam, Ali Hossein Rezayan, Ali Poorkhalil, Seyed Hossein Ahmadi Tafti, Asieh Heirani-Tabasi, Hossein Eyni, Marjan Malekmohamadi, Safieh Boroumand, Alessandra Pinna
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引用次数: 0

摘要

在推进心脏组织工程(CTE)中,开发反映心肌特性的可注射水凝胶是关键。所设计的水凝胶不仅要支持心脏细胞的生长,而且要有导电性,以适当促进心脏细胞的功能。本研究开发了一种简便的方法,将金纳米颗粒(AuNPs)掺入由海藻酸盐(Alg)和明胶(Gel)组成的可注射水凝胶中。所得的纳米复合水凝胶具有多孔互联网络,与碱性Alg/Gel水凝胶相比,其电导率(2.04 × 10-4 S cm-1)更高。水凝胶水合作用和体外降解谱证实了它们作为心脏细胞载体的适用性。重要的是,与对照组相比,Alg/Gel+AuNPs水凝胶在7天内对小鼠胚胎心肌细胞(mECCs)没有毒性,升高了连接蛋白43 (Cx43)和心肌肌钙蛋白T (CTnT)基因表达。然后,将Alg/Gel+AuNPs水凝胶作为载体,在心肌梗死大鼠心内递送mecc。α-平滑肌肌动蛋白(α-SMA)和心肌肌钙蛋白T (CTnT)的表达随着射血分数(EF)的增加而显著升高,梗死面积变小,纤维化面积减小,证实水凝胶有效促进了移植细胞与周围组织之间的机械电信号传递。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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An Alginate/Gelatin Injectable Hydrogel Containing Au Nanoparticles for Transplantation of Embryonic Mouse Cardiomyocytes in Myocardial Repair

In advancing cardiac tissue engineering (CTE), the development of injectable hydrogels mirroring myocardial properties is pivotal. The designed hydrogels must not only support cardiac cell growth but also have to be conductive to properly promote the functionalities of cardiac cells. Here, a facile approach is developed to incorporate gold nanoparticles (AuNPs) into an injectable hydrogel composed of Alginate (Alg) and Gelatin (Gel). The resultant nanocomposite hydrogel boasts a porous interconnected network and superior conductivity (2.04 × 10–4 S cm−1) compared to the base Alg/Gel hydrogel. Hydrogel hydration and in vitro degradation profiles affirm their suitability as carriers for cardiac cells. Importantly, Alg/Gel+AuNPs hydrogels exhibit no toxicity to mouse Embryonic Cardiac Cells (mECCs) over 7 days, elevating connexin 43 (Cx43) and cardiac troponin T (CTnT) gene expression compared to controls. Then, the Alg/Gel+AuNPs hydrogel is used as a carrier for intramyocardial delivery of mECCs in rats with myocardial infarction. The significant increase in α-Smooth Muscle Actin (α-SMA) and cardiac troponin T (CTnT) expression along with the increase in ejection fraction (EF), smaller infarction size, less fibrosis area confirmed that the hydrogel efficiently promoted the transmission of mechanical and electrical signals between transplanted cells and surrounding tissue.

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来源期刊
Macromolecular bioscience
Macromolecular bioscience 生物-材料科学:生物材料
CiteScore
7.90
自引率
2.20%
发文量
211
审稿时长
1.5 months
期刊介绍: Macromolecular Bioscience is a leading journal at the intersection of polymer and materials sciences with life science and medicine. With an Impact Factor of 2.895 (2018 Journal Impact Factor, Journal Citation Reports (Clarivate Analytics, 2019)), it is currently ranked among the top biomaterials and polymer journals. Macromolecular Bioscience offers an attractive mixture of high-quality Reviews, Feature Articles, Communications, and Full Papers. With average reviewing times below 30 days, publication times of 2.5 months and listing in all major indices, including Medline, Macromolecular Bioscience is the journal of choice for your best contributions at the intersection of polymer and life sciences.
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