Guizhi Feng, Hongmei Liu, Chengyan Deng, Dan Liu, Yingtian Guo
{"title":"PtaGRF和PtaGIF家族的全基因组研究揭示了油松球果发育和赤霉素信号传导的潜在靶点","authors":"Guizhi Feng, Hongmei Liu, Chengyan Deng, Dan Liu, Yingtian Guo","doi":"10.1007/s00468-024-02580-z","DOIUrl":null,"url":null,"abstract":"<div><h3>Key message</h3><p>We identified 7 <i>PtaGRF</i> and 3 <i>PtaGIF</i> genes and analyzed the potential functions of PtaGRF02-PtaGIF02 that may mediate cone development and gibberellin signaling in Chinese pine.</p><h3>Abstract</h3><p>Growth-regulating factors (GRFs) and GRF-interacting factors (GIFs) act as transcription cofactors that influence plant growth and development. While these genes have been extensively studied in various plant species, a comprehensive genome-wide analysis of <i>GRFs</i> and <i>GIFs</i> in conifers has been lacking. In this study, we identified 7 <i>PtaGRFs</i> and 3 <i>PtaGIFs</i> in Chinese pine (<i>Pinus tabuliformis</i>) and conducted a detailed analysis of their motifs, domains, structures, and regulatory elements. Phylogenetic analysis revealed that GRF proteins grouped into five clades, with PtaGRFs forming a single clade. We further investigated chromosome mapping and collinearity. Through expression profiling and correlation analysis, <i>PtaGRF02</i> and <i>PtaGIF02</i> were excavated, which may play a role in cone development. Protein–protein interaction analysis indicated that these two factors could interact with proteins involved in flower development. Moreover, PtaGRF02 and PtaGIF02 were shown to interact with each other through yeast two-hybrid and BiFC assays, and they were found to interact with PtaDELLAs to modulate gibberellin (GA) signaling. Overall, our findings offer valuable genetic insights into reproductive development in conifers.</p></div>","PeriodicalId":805,"journal":{"name":"Trees","volume":"39 1","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genome-wide investigation of the PtaGRF and PtaGIF families reveals potential targets for cone development and gibberellin signaling in Chinese pine\",\"authors\":\"Guizhi Feng, Hongmei Liu, Chengyan Deng, Dan Liu, Yingtian Guo\",\"doi\":\"10.1007/s00468-024-02580-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Key message</h3><p>We identified 7 <i>PtaGRF</i> and 3 <i>PtaGIF</i> genes and analyzed the potential functions of PtaGRF02-PtaGIF02 that may mediate cone development and gibberellin signaling in Chinese pine.</p><h3>Abstract</h3><p>Growth-regulating factors (GRFs) and GRF-interacting factors (GIFs) act as transcription cofactors that influence plant growth and development. While these genes have been extensively studied in various plant species, a comprehensive genome-wide analysis of <i>GRFs</i> and <i>GIFs</i> in conifers has been lacking. In this study, we identified 7 <i>PtaGRFs</i> and 3 <i>PtaGIFs</i> in Chinese pine (<i>Pinus tabuliformis</i>) and conducted a detailed analysis of their motifs, domains, structures, and regulatory elements. Phylogenetic analysis revealed that GRF proteins grouped into five clades, with PtaGRFs forming a single clade. We further investigated chromosome mapping and collinearity. Through expression profiling and correlation analysis, <i>PtaGRF02</i> and <i>PtaGIF02</i> were excavated, which may play a role in cone development. Protein–protein interaction analysis indicated that these two factors could interact with proteins involved in flower development. Moreover, PtaGRF02 and PtaGIF02 were shown to interact with each other through yeast two-hybrid and BiFC assays, and they were found to interact with PtaDELLAs to modulate gibberellin (GA) signaling. Overall, our findings offer valuable genetic insights into reproductive development in conifers.</p></div>\",\"PeriodicalId\":805,\"journal\":{\"name\":\"Trees\",\"volume\":\"39 1\",\"pages\":\"\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-12-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Trees\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00468-024-02580-z\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FORESTRY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trees","FirstCategoryId":"2","ListUrlMain":"https://link.springer.com/article/10.1007/s00468-024-02580-z","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FORESTRY","Score":null,"Total":0}
Genome-wide investigation of the PtaGRF and PtaGIF families reveals potential targets for cone development and gibberellin signaling in Chinese pine
Key message
We identified 7 PtaGRF and 3 PtaGIF genes and analyzed the potential functions of PtaGRF02-PtaGIF02 that may mediate cone development and gibberellin signaling in Chinese pine.
Abstract
Growth-regulating factors (GRFs) and GRF-interacting factors (GIFs) act as transcription cofactors that influence plant growth and development. While these genes have been extensively studied in various plant species, a comprehensive genome-wide analysis of GRFs and GIFs in conifers has been lacking. In this study, we identified 7 PtaGRFs and 3 PtaGIFs in Chinese pine (Pinus tabuliformis) and conducted a detailed analysis of their motifs, domains, structures, and regulatory elements. Phylogenetic analysis revealed that GRF proteins grouped into five clades, with PtaGRFs forming a single clade. We further investigated chromosome mapping and collinearity. Through expression profiling and correlation analysis, PtaGRF02 and PtaGIF02 were excavated, which may play a role in cone development. Protein–protein interaction analysis indicated that these two factors could interact with proteins involved in flower development. Moreover, PtaGRF02 and PtaGIF02 were shown to interact with each other through yeast two-hybrid and BiFC assays, and they were found to interact with PtaDELLAs to modulate gibberellin (GA) signaling. Overall, our findings offer valuable genetic insights into reproductive development in conifers.
期刊介绍：
Trees - Structure and Function publishes original articles on the physiology, biochemistry, functional anatomy, structure and ecology of trees and other woody plants. Also presented are articles concerned with pathology and technological problems, when they contribute to the basic understanding of structure and function of trees. In addition to original articles and short communications, the journal publishes reviews on selected topics concerning the structure and function of trees.
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