Shruti A Bhamare, Prasanna T Dahake, Yogesh J Kale, Mahesh V Dadpe, Shrikant B Kendre
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The inhibition efficacy of both agents was determined by biofilm adhesion reduction crystal violet assay.</p><p><strong>Results: </strong>The MIC of SA was 25 μg/mL, and for CO, it was 12.5 μg/mL. The time-kill assay revealed that antibacterial efficacy was identified till 36 hours by both the test materials. The mean biofilm reduction at 25 µg/mL of calcium hydroxide [Ca(OH)<sub>2</sub>], SA, and CO was 1.53 ± 0.05, 1.83 ± 1.57, and 2.06 ± 0.05, respectively.</p><p><strong>Conclusion: </strong>SA and CO demonstrated promising antibacterial efficacy against <i>E. faecalis</i> and CO presented significant eradication of biofilms compared to SA.</p><p><strong>How to cite this article: </strong>Bhamare SA, Dahake PT, Kale YJ, <i>et al.</i> Effect of Herbal Extract of <i>Spilanthes acmella</i> and Cinnamon Oil on <i>Enterococcus faecalis</i> Biofilm Eradication: An <i>In Vitro</i> Study. 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引用次数: 0
摘要
粪肠球菌在牙髓治疗失败中起致病作用。本研究旨在评价香泼草（SA）和肉桂油（CO）提取物对粪肠球菌生物膜的根除效果。材料与方法：采用最小抑菌浓度（MIC）和最小杀菌浓度（MBC）试验评价SA和CO对粪肠球菌的抑菌效果，并采用时间杀伤法评价其与不同时间间隔的相互作用。采用生物膜粘附还原结晶紫法测定两种药剂的抑菌效果。结果：SA的MIC为25 μg/mL， CO的MIC为12.5 μg/mL。时间杀伤试验表明，两种材料的抗菌效果均可持续到36小时。25µg/mL氢氧化钙[Ca(OH)2]、SA和CO的平均生物膜还原量分别为1.53±0.05、1.83±1.57和2.06±0.05。结论：SA和CO对粪肠球菌具有良好的抗菌作用，CO对粪肠球菌生物膜的清除作用明显强于SA。如何引用本文：Bhamare SA， Dahake PT， Kale YJ等。花楸提取物和肉桂油对粪肠球菌生物膜根除作用的体外研究。中华临床儿科杂志；2017;17(9):1004-1013。
Effect of Herbal Extract of Spilanthes acmella and Cinnamon Oil on Enterococcus faecalis Biofilm Eradication: An In Vitro Study.
Introduction: Enterococcus faecalis has a pathogenic role in failed endodontic treatments. The study aimed to assess the efficiency of Spilanthesacmella (SA) and cinnamon oil (CO) extract on E. faecalis biofilm eradication.
Materials and methods: The antibacterial efficacy of SA and CO against E. faecalis was assessed by the tests of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), and further, the interaction with agents was evaluated at different time intervals by a time-kill assay. The inhibition efficacy of both agents was determined by biofilm adhesion reduction crystal violet assay.
Results: The MIC of SA was 25 μg/mL, and for CO, it was 12.5 μg/mL. The time-kill assay revealed that antibacterial efficacy was identified till 36 hours by both the test materials. The mean biofilm reduction at 25 µg/mL of calcium hydroxide [Ca(OH)2], SA, and CO was 1.53 ± 0.05, 1.83 ± 1.57, and 2.06 ± 0.05, respectively.
Conclusion: SA and CO demonstrated promising antibacterial efficacy against E. faecalis and CO presented significant eradication of biofilms compared to SA.
How to cite this article: Bhamare SA, Dahake PT, Kale YJ, et al. Effect of Herbal Extract of Spilanthes acmella and Cinnamon Oil on Enterococcus faecalis Biofilm Eradication: An In Vitro Study. Int J Clin Pediatr Dent 2024;17(9):1004-1013.
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