牙本质胶原中积累的晚期糖基化终产物（AGEs）的测量。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Oral Biosciences Pub Date : 2024-12-12 DOI:10.1016/j.job.2024.100600

Ruri Asami, Takuya Sato, Koji Sakiyama

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引用次数: 0

摘要

目的：在美拉德反应中，还原糖以非酶的方式与蛋白质结合，最终产物被统称为晚期糖基化终产物（age）。AGEs在人体内随着年龄的增长而积累，并引起组织和细胞的各种紊乱。本研究利用一种可以稳定测量的年龄——戊苷，研究了牙本质内戊苷积累的差异，以及结果是否可以指示年龄。方法：采用高效液相色谱法测定无龋病史的拔牙牙本质胶原中积累的AGE戊苷。结果：牙本质戊苷浓度随年龄增长而增加，牙釉质-牙本质交界处的戊苷浓度高于牙髓腔。牙本质戊苷水平与年龄密切相关。结论：这些发现表明测量牙本质胶原中AGEs积累作为年龄指标的效用。

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Measurement of advanced glycation end products (AGEs) accumulated in dentin collagen

Objective

In the Maillard reaction, reducing sugars bind to proteins in a non-enzymatic manner, and the final products are collectively named, advanced glycation end products (AGEs). AGEs accumulate in the human body with age and cause various disorders in tissues and cells. In this study, pentosidine, an AGE that can be stably measured, was used to investigate the differences in pentosidine accumulation within dentin, and whether the results can indicate age.

Methods

High-performance liquid chromatography was used to measure pentosidine, an AGE that accumulates in dentin collagen, in extracted teeth with no caries and a clear history.

Results

The dentin pentosidine concentration increased with age, with a higher pentosidine concentration observed at the enamel-dentin junction aspect than at the pulp cavity aspect. A close correlation was observed between the dentin pentosidine level and age.