透明质酸凝胶单独或联合葡萄柚籽提取物对Wistar大鼠下颌磨牙诱导牙周炎的潜在抗炎作用。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Oral Biosciences Pub Date : 2024-12-12 DOI:10.1016/j.job.2024.100598

Sana Mostafa , Aiah A. El-Rashidy , Mohamed Talaat Elbehwashy , Manar A. Abdul-Aziz , Nermeen AbuBakr

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引用次数: 0

摘要

目的：抗菌药物与常规化学机械治疗相结合，以改善牙周炎的治疗效果。本研究旨在评估局部应用透明质酸（HA）加或不加葡萄柚籽提取物（GFSE）（5%、10%和15% wt. %）对大鼠诱导牙周炎的改善作用。方法：用30只成年雄性Wistar大鼠造牙槽骨缺损，用大肠杆菌脂多糖浸渍结扎法诱导牙周炎4周。将大鼠分为5组（n = 6）；牙周炎未治疗组和四个治疗组，每周向龈沟注射凝胶（HA +/- GFSE）一次，持续两周。实验开始6周后，对所有大鼠实施安乐死，制备下颌骨进行组织病理学和组织形态学分析。采用酶联免疫吸附法测定组织中肿瘤坏死因子-α (TNF-α)、转化生长因子-β1 （TGF-β1）、对氧磷酶-1 （PON-1）酶水平。结果：透明质酸促进缺损边缘的新骨形成，从而减小缺损宽度。当HA与GFSE以剂量依赖的方式联合使用时，这种效果显著增加。此外，HA和GFSE混合物抑制TNF-α和TGF-β1的组织水平，从而增加PON-1。结论：透明质酸与GFSE合剂治疗慢性牙周炎具有一定的剂量依赖性。

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The potential anti-inflammatory effect of hyaluronic acid gel alone or in combination with grapefruit seed extract on induced periodontitis in mandibular molars of Wistar rats

Objectives

Antimicrobial agents have been used in conjunction with conventional chemomechanical therapy to improve the treatment outcomes of periodontitis. This study aimed to evaluate the ameliorating effect of topical application of hyaluronic acid (HA) with or without grapefruit seed extract (GFSE) (5, 10, and 15 wt %) in induced periodontitis in rats.

Methods

Surgical alveolar bone defects were created in 30 adult male Wistar rats, followed by the introduction of a ligature impregnated with Escherichia coli lipopolysaccharide for four weeks to induce periodontitis. Rats were distributed into five groups (n = 6); an untreated periodontitis group and four treated groups in which gel (HA±GFSE) was injected into the sulcus once weekly for two weeks. All rats were euthanized six weeks after starting the experiment, and the mandibles were prepared for histopathological and histomorphometric analyses. Enzyme-linked immunosorbent assay was used for measuring tissue levels of tumor necrosis factor-alpha (TNF-α), transforming growth factor-β1 (TGF-β1), and paraoxonase-1 (PON-1) enzyme.

Results

HA enhanced new bone formation at defect margins, thereby diminishing defect width. This effect significantly increased as HA was combined with GFSE in a dose-dependent manner. Moreover, the HA and GFSE mixture suppressed the tissue levels of TNF-α and TGF-β1, thereby increasing PON-1.