利用小分子-蛋白杂交荧光探针定量溶酶体可溶性 Zn2+ 并监测 Zn2+ 外流。

IF 3.8 2区化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Inorganic Biochemistry Pub Date : 2025-03-01 Epub Date: 2024-12-10 DOI:10.1016/j.jinorgbio.2024.112811

Yuyin Du, Toshiyuki Kowada, EunHye Sung, Rong Liu, Andrei Soloviev, Toshitaka Matsui, Shin Mizukami

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引用次数: 0

摘要

溶酶体不稳定的Zn2+水平尚不清楚。通过VAMP7-Halo将ZnDA-3H小分子荧光探针靶向溶酶体，测定HeLa细胞中溶酶体不稳定Zn2+浓度为1.9 nM。此外，ZnDA-3H可以直接可视化trpml激活时溶酶体向胞质中流出的Zn2+。

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Quantification of lysosomal labile Zn²⁺ and monitoring of Zn²⁺ efflux using a small-molecule-protein hybrid fluorescent probe.

Lysosomal labile Zn²⁺ levels have been unclear. By targeting a small-molecule fluorescent Zn²⁺ probe, ZnDA-3H, to lysosomes via VAMP7-Halo, the lysosomal labile Zn²⁺ concentration was determined to be 1.9 nM in HeLa cells. Furthermore, ZnDA-3H enabled direct visualization of the Zn²⁺ efflux from the lysosomes to cytosol upon TRPMLs activation.

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来源期刊

Journal of Inorganic Biochemistry 生物-生化与分子生物学

CiteScore

7.00

自引率

10.30%

发文量

336

审稿时长

41 days

期刊介绍： The Journal of Inorganic Biochemistry is an established international forum for research in all aspects of Biological Inorganic Chemistry. Original papers of a high scientific level are published in the form of Articles (full length papers), Short Communications, Focused Reviews and Bioinorganic Methods. Topics include: the chemistry, structure and function of metalloenzymes; the interaction of inorganic ions and molecules with proteins and nucleic acids; the synthesis and properties of coordination complexes of biological interest including both structural and functional model systems; the function of metal- containing systems in the regulation of gene expression; the role of metals in medicine; the application of spectroscopic methods to determine the structure of metallobiomolecules; the preparation and characterization of metal-based biomaterials; and related systems. The emphasis of the Journal is on the structure and mechanism of action of metallobiomolecules.