膝关节牵张后再生软骨的蛋白质组学特征人类案例研究。

IF 2.8 4区 医学 Q3 CELL BIOLOGY Connective Tissue Research Pub Date : 2024-11-01 Epub Date: 2024-12-17 DOI:10.1080/03008207.2024.2440716
Jessica S J J Steijns, Daniel Green, Laura C W Peeters, Pieter J Emans, Tim A Boymans, Roderick H Stassen, Guus G H van den Akker, Andy Cremers, Liesbeth M C Jutten, James R Anderson, Mandy J Peffers, Marjolein M J Caron, Tim J M Welting
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引用次数: 0

摘要

目的:膝关节牵张术是一种具有软骨再生特性的外科手术。人类患者关节牵张再生软骨的组成文献很少。在这个案例研究中,我们提供了一个独特的机会,从生物分子特征的再生组织,从患者接受双侧撑开和后来的膝关节置换术。方法:采用外固定架撑开膝关节,几年后行全膝关节置换术。在临床干预之前、期间和之后进行影像学检查以评估膝关节的状况。全膝关节置换术后,收集软骨活检并进行组织切片和组织化学染色。串联质谱蛋白质组学分析用于表征和比较蛋白质组学组成。结果:膝关节牵张前后,双膝关节关节间隙均有改善。再生软骨呈白色,表面不规则,而原生(侧)软骨外观为黄色,表面光滑。组织化学染色显示,与再生软骨相比,天然软骨中的红花素- o阳性较高,胶原结构也存在差异。蛋白质组学分析没有显示软骨细胞外基质蛋白丰度的主要差异。生物信息学分析显示核糖体蛋白(再生软骨)和RNA聚合酶II转录终止(天然软骨)富集。结论:组织学上,膝关节牵张再生软骨与天然软骨相比,糖胺聚糖和胶原组织紊乱较少。然而,质谱分析没有发现细胞外基质蛋白丰度的主要差异,蛋白质组学线索提示蛋白质翻译调节是再生的潜在机制。
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Proteomic characterization of regenerated cartilage following knee joint distraction; a human case-study.

Purpose: Knee joint distraction is a surgical procedure with cartilage-regenerating properties. The composition of joint distraction-regenerated cartilage in human patients is poorly documented. In this case-study, provided a unique opportunity to biomolecularly characterize the regenerated tissue from a patient who underwent bilateral distraction and later knee replacements.

Methods: Knee joint distraction was conducted using an external fixation frame and total knee arthroplasty was performed several years later. Radiographic imaging was performed to assess the status of the knee joint prior, during and after clinical interventions. Following total knee replacement, cartilage biopsies were collected and processed for tissue sectioning and histochemical staining. Tandem mass-spectrometry proteomics analysis was used to characterize and compare the proteomic composition.

Results: Both knee joints showed joint-space improvement pre- and post-knee joint distraction. Regenerated cartilage was white with an irregular surface, while native (lateral) cartilage had a yellow appearance and smooth surface. Histochemical staining showed higher Safranin-O positivity in native cartilage compared to regenerated cartilage, and differences in collagen structure. Proteomic analysis did not reveal major differences in cartilage extracellular matrix protein abundance. Bioinformatic analyses revealed enrichment in ribosomal proteins (regenerated cartilage) and RNA Polymerase II Transcription Termination (native cartilage).

Conclusion: Histologically, knee joint distraction-regenerated cartilage showed less glycosaminoglycans and disorganized collagen compared to native cartilage. However, mass-spectrometry has no major differences in extracellular matrix protein abundance, with proteomic clues suggesting protein translation regulation as a potential mechanism for regeneration.

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来源期刊
Connective Tissue Research
Connective Tissue Research 生物-细胞生物学
CiteScore
6.60
自引率
3.40%
发文量
37
审稿时长
2 months
期刊介绍: The aim of Connective Tissue Research is to present original and significant research in all basic areas of connective tissue and matrix biology. The journal also provides topical reviews and, on occasion, the proceedings of conferences in areas of special interest at which original work is presented. The journal supports an interdisciplinary approach; we present a variety of perspectives from different disciplines, including Biochemistry Cell and Molecular Biology Immunology Structural Biology Biophysics Biomechanics Regenerative Medicine The interests of the Editorial Board are to understand, mechanistically, the structure-function relationships in connective tissue extracellular matrix, and its associated cells, through interpretation of sophisticated experimentation using state-of-the-art technologies that include molecular genetics, imaging, immunology, biomechanics and tissue engineering.
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