兔喉气管狭窄生存模型的建立：一项前瞻性随机研究

IF 1.6 4区医学 Q2 OTORHINOLARYNGOLOGY Laryngoscope Investigative Otolaryngology Pub Date : 2024-12-20 DOI:10.1002/lio2.70047

Wei Chen MD, Qingyu Wang MM, Hongming Xu MD, Yuhui Xie MM, Lina Zhang MM, Yao Li PhD, Guofeng Yan PhD, Yiwen Ding PhD, Shunkai Lu MM, Zhibo Xie MD, Jiarui Chen MD, Mengrou Xu MD, Xiaoben Liang MD, Juan Chen PhD, Penghuai Fu PhD, Xiaoyan Li MD, PhD, Liming Peng PhD

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引用次数: 0

摘要

目的建立可重复生存的兔喉气管狭窄模型。方法将70只新西兰白兔随机分为试验组（n = 30）和对照组（n = 40）。在实验组中，在纤维喉镜（FOL）的辅助下，从气管切开术逆行插入尼龙刷，穿过声门下，旋转至软骨暴露的全层环周粘膜损伤。实验组1 (n = 10)，旋转10次；第二组（n = 20），旋转20次。对照组仅行气管切开术，不使用尼龙刷刮拭。分别于伤后第1、4、8、12周行FOL观察LTS的形成。对大鼠实施安乐死，并于伤后12周对其喉部和气管进行大体和组织病理学检查。结果对照组全部存活，实验组5例死于LTS和/或粘液堵塞。组织学观察显示，对照组喉气管粘膜上皮完整，无狭窄；实验组成纤维细胞增生，胶原纤维增厚。对照组平均狭窄率为9.31±0.98%，实验组1平均狭窄率为32.78±7.07%，实验组2平均狭窄率为58.25±8.96%。三组间差异有统计学意义（χ2 = 47.98, p < 0.05）。结论采用尼龙刷法，通过FOL可视化结合气管切开术，成功建立了可重复的兔LTS存活模型。该模型可为探索创面愈合病理生理及干预效果提供成熟稳定的动物模型。证据等级NA。

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Establishment of a survival rabbit model for laryngotracheal stenosis: A prospective randomized study

Objective

To develop a reproducible survival rabbit model for laryngotracheal stenosis (LTS).

Methods

Seventy New Zealand white (NZW) rabbits were randomly divided into experimental groups (n = 30) and a control group (n = 40). In experimental groups, a nylon brush was inserted retrograde from the tracheotomy through the subglottis and rotated until a full layer circumferential mucosal injury to cartilage exposure, assisted by fiberoptic laryngoscopy (FOL) visualization. Experimental group 1 (n = 10), rotated 10 times; group 2 (n = 20), rotated 20 times. The control group underwent tracheotomy only without nylon brush scraping. The rabbits underwent FOL at 1st, 4th, 8th, and 12th week postinjury respectively to observe the formation of LTS. They were euthanized and the larynxes and tracheas were subjected to gross and histopathological examination at 12 weeks postinjury.

Results

The control group all survived, while five cases in experimental groups died from LTS and/or mucous plug. Histological observation showed that the control group had intact laryngotracheal mucosal epithelium without any stenosis; the experimental groups showed proliferation of fibroblasts and thickening of collagen fibers. The mean stenosis in control group was 9.31 ± 0.98%, while that in experimental group 1 was 32.78 ± 7.07% and 58.25 ± 8.96% in experimental group 2. The difference between the three groups was statistically significant (χ² = 47.98, p < .05).

Conclusions

We successfully developed a reproducible survival rabbit model for LTS using a nylon brush through FOL visualization combined with tracheostomy. This model can provide a mature and stable animal model for the exploration of wound-healing pathophysiology and the effect of interventions.