{"title":"内生真菌发酵镰刀菌SME13-2中镰刀菌酸和9,10-脱氢镰刀菌酸对细胞壁和膜完整性破坏的抑菌和抗真菌作用模式","authors":"Martha Lydia Macías-Rubalcava, Patricia Lappe-Oliveras, Rodrigo Villanueva-Silva","doi":"10.1093/jambio/lxae301","DOIUrl":null,"url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to assess the mode of action of fusaric and 9,10-dehydrofusaric acids on cell respiration by measuring the hyphal oxygen consumption rate, and the effects on cell membrane integrity by determining the electrical conductivity of the mycelium.</p><p><strong>Methods and results: </strong>Bioactivity-directed fractionation of the active culture medium and mycelium organic extracts from the Fusarium lactis strain SME13-2 isolated from Sapium macrocarpum led to the isolation of two known alkylpicolinic acid derivatives: fusaric acid and 9,10-dehydrofusaric acid, along with the known polyketide bikaverin. Fusaric acid and 9,10-dehydrofusaric acid exhibited antioomycete and antifungal activities, significantly inhibiting the radial growth of Phytophthora capsici, Pythium aphanidermatum, Alternaria alternata, and F. oxysporum. Additionally, they induced changes in colony morphology and negative effects on the ultrastructure of their hyphae. To date, the antimicrobial mode of action of fusaric acid and other alkylpicolinic acid derivatives is not thoroughly understood. Therefore, we investigated their effect on cellular respiration by measuring the oxygen consumption rate of the hyphae and their impact on cell membrane integrity by determining the electrical conductivity of the mycelium. Fusaric and 9,10-dehydrofusaric acids inhibited the respiration of the mycelium and altered the permeability of the cell membrane in the tested phytopathogenic microorganisms in a concentration and exposure time-dependent manner, exerting a greater effect on oomycetes. The disruption of cell membrane integrity resulted in the leakage of cytoplasmic electrolytes into the extracellular medium, which, coupled with respiratory inhibition, could lead to cell death.</p><p><strong>Conclusions: </strong>Fusaric and 9,10-dehydrofusaric acids show potential for the development of new fungicides and anti-oomycetes agents.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Disruption of cell wall and membrane integrity as antioomycete and antifungal mode of action by fusaric and 9,10-dehydrofusaric acids from endophytic fungus Fusarium lactis strain SME13-2.\",\"authors\":\"Martha Lydia Macías-Rubalcava, Patricia Lappe-Oliveras, Rodrigo Villanueva-Silva\",\"doi\":\"10.1093/jambio/lxae301\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aims: </strong>This study aimed to assess the mode of action of fusaric and 9,10-dehydrofusaric acids on cell respiration by measuring the hyphal oxygen consumption rate, and the effects on cell membrane integrity by determining the electrical conductivity of the mycelium.</p><p><strong>Methods and results: </strong>Bioactivity-directed fractionation of the active culture medium and mycelium organic extracts from the Fusarium lactis strain SME13-2 isolated from Sapium macrocarpum led to the isolation of two known alkylpicolinic acid derivatives: fusaric acid and 9,10-dehydrofusaric acid, along with the known polyketide bikaverin. Fusaric acid and 9,10-dehydrofusaric acid exhibited antioomycete and antifungal activities, significantly inhibiting the radial growth of Phytophthora capsici, Pythium aphanidermatum, Alternaria alternata, and F. oxysporum. Additionally, they induced changes in colony morphology and negative effects on the ultrastructure of their hyphae. To date, the antimicrobial mode of action of fusaric acid and other alkylpicolinic acid derivatives is not thoroughly understood. Therefore, we investigated their effect on cellular respiration by measuring the oxygen consumption rate of the hyphae and their impact on cell membrane integrity by determining the electrical conductivity of the mycelium. Fusaric and 9,10-dehydrofusaric acids inhibited the respiration of the mycelium and altered the permeability of the cell membrane in the tested phytopathogenic microorganisms in a concentration and exposure time-dependent manner, exerting a greater effect on oomycetes. The disruption of cell membrane integrity resulted in the leakage of cytoplasmic electrolytes into the extracellular medium, which, coupled with respiratory inhibition, could lead to cell death.</p><p><strong>Conclusions: </strong>Fusaric and 9,10-dehydrofusaric acids show potential for the development of new fungicides and anti-oomycetes agents.</p>\",\"PeriodicalId\":15036,\"journal\":{\"name\":\"Journal of Applied Microbiology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-01-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1093/jambio/lxae301\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/jambio/lxae301","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Disruption of cell wall and membrane integrity as antioomycete and antifungal mode of action by fusaric and 9,10-dehydrofusaric acids from endophytic fungus Fusarium lactis strain SME13-2.
Aims: This study aimed to assess the mode of action of fusaric and 9,10-dehydrofusaric acids on cell respiration by measuring the hyphal oxygen consumption rate, and the effects on cell membrane integrity by determining the electrical conductivity of the mycelium.
Methods and results: Bioactivity-directed fractionation of the active culture medium and mycelium organic extracts from the Fusarium lactis strain SME13-2 isolated from Sapium macrocarpum led to the isolation of two known alkylpicolinic acid derivatives: fusaric acid and 9,10-dehydrofusaric acid, along with the known polyketide bikaverin. Fusaric acid and 9,10-dehydrofusaric acid exhibited antioomycete and antifungal activities, significantly inhibiting the radial growth of Phytophthora capsici, Pythium aphanidermatum, Alternaria alternata, and F. oxysporum. Additionally, they induced changes in colony morphology and negative effects on the ultrastructure of their hyphae. To date, the antimicrobial mode of action of fusaric acid and other alkylpicolinic acid derivatives is not thoroughly understood. Therefore, we investigated their effect on cellular respiration by measuring the oxygen consumption rate of the hyphae and their impact on cell membrane integrity by determining the electrical conductivity of the mycelium. Fusaric and 9,10-dehydrofusaric acids inhibited the respiration of the mycelium and altered the permeability of the cell membrane in the tested phytopathogenic microorganisms in a concentration and exposure time-dependent manner, exerting a greater effect on oomycetes. The disruption of cell membrane integrity resulted in the leakage of cytoplasmic electrolytes into the extracellular medium, which, coupled with respiratory inhibition, could lead to cell death.
Conclusions: Fusaric and 9,10-dehydrofusaric acids show potential for the development of new fungicides and anti-oomycetes agents.
期刊介绍：
Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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