[携带具有调节聚糖组成的HIV-1 Env的病毒样颗粒]。

G A Kaevitser, E I Samokhvalov, D V Scheblyakov, A L Gintsburg, A N Vzorov
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引用次数: 0

摘要

先前获得的高免疫原性Env-VLPs确保克服HIV-1表面蛋白的自然抗性,这与它们的低结合水平和保守表位的不可接近性有关,从而诱导中和抗体。我们还采用该技术修饰ZM53(T/F)株的Env三聚体,通过重组痘苗病毒(rVVs)产生Env- vlps。为了生产VLP,使用了表达Env, Gag-Pol (HIV-1/SIV)和牛痘病毒hr基因的rvv,该基因克服了牛痘病毒在CHO细胞中复制的限制。缺乏GlcNAc-TI的CHO Lec1工程细胞系用于产生含有影响表面亚基(SU)构象的细胞质(CT)结构域的Env蛋白的VLPs。这为调节聚糖组成、完善其生产条件以及优化克服与大量糖基化相关的HIV-1耐药性的方法创造了机会。
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[Virus-Like Particles Carrying HIV-1 Env with a Modulated Glycan Composition].

Previously obtained highly immunogenic Env-VLPs ensure overcoming the natural resistance of HIV-1 surface proteins associated with their low level of incorporation and inaccessibility of conserved epitopes to induce neutralizing antibodies. We also adopted this technology to modify Env trimers of the ZM53(T/F) strain to produce Env-VLPs by recombinant vaccinia viruses (rVVs). For VLP production, rVVs expressing Env, Gag-Pol (HIV-1/SIV), and the cowpox virus hr gene, which overcomes the restriction of vaccinia virus replication in CHO cells, were used. The CHO Lec1 engineered cell line lacking GlcNAc-TI was used for generating VLPs with Env proteins containing a cytoplasmic (CT) domain affecting the surface subunit (SU) conformation. This has created the opportunity to modulate the glycan composition, and refine the conditions for their production, and optimize approaches to overcoming HIV-1 resistance associated with abundant glycosylation.

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来源期刊
Molekulyarnaya Biologiya
Molekulyarnaya Biologiya Medicine-Medicine (all)
CiteScore
0.70
自引率
0.00%
发文量
131
期刊最新文献
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